Proteome alterations of reverse photoperiod-sensitive genic male sterile rice (Oryza sativa L.) at fertility transformation stage
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The two-line system has been widely used in hybrid rice seed production, and the reverse photoperiod-sensitive genic male sterile line is a new germplasm with an opposite phenotype compared with normal photoperiod-sensitive genic male sterile rice. To better understand the molecular mechanisms of fertility regulation in reverse photoperiod-sensitive genic male sterile rice, a comparative proteomic approach was used to analyze the protein profiles of three different tissues (young panicles, flag leaves and leaf sheaths) of D52S during the sensitive period of pollen fertility transformation under sterile and fertile conditions. By quantitative analysis, 66 protein spots were identified to be significantly changed in the three tissues. Bioinformatics analyses revealed that in sterile rice, a number of proteins involved in lignin-flavonoid biosynthesis pathway were down-accumulated in panicles. The majority of proteins associated with energy metabolism were down-accumulated in leaf sheaths while the proteins up-accumulated in leaves and leaf sheaths were exclusively photosynthesis and defense related. Based on the proteomics data, a short-day induced male sterility protein network was proposed. In addition, the genes of selected protein spots were further analyzed by qPCR. These findings provide data for better understanding the regulation of pollen fertility in reverse photoperiod-sensitive genic male sterile rice, which could assist in the development of practical reverse photoperiod-sensitive genic male sterile rice for large-scale crop breeding programs.
Keywords2-DE Fertility Plant proteomics Reverse photoperiod-sensitive genic male sterile rice
Reverse photoperiod-sensitive genic male sterile
Environmentally sensitive genetic male sterile
Photoperiod-sensitive genic male sterile
Thermo-sensitive genic male sterile
This work was supported by the National Natural Science Foundation of China (Grant No. 31371600).
Conflict of interest
The authors have declared no conflict of interest.
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