Genes & Genomics

, Volume 35, Issue 1, pp 125–130 | Cite as

Triglyceride (TG) down-regulates expression of MCP-1 and CCR2 in PMA-derived THP-1 macrophages

  • Yoon Suk Kim
  • Ho Joong Sung
  • Sin Jee Son
  • Jaewon Lim
  • Yeo Wool Kang
  • Tae Ue Kim
  • Ki-Jong RheeEmail author
Research Article


Triglycerides (TGs) are implicated in the development of atherosclerosis. A key contributing factor for atherosclerosis is the migration of macrophages to atherosclerotic lesions. MCP-1 is a major chemoattractant for macrophages to atherosclerotic lesions. We examined the expression profile of MCP-1 and CCR2 in THP-1 macrophages in response to TG treatment by RT-PCR analysis. Chemical inhibitors were used to identify cell signaling pathway(s) involved in regulation of MCP-1 and CCR2 expression. We found that treatment of THP-1 macrophages with TG down-regulated MCP-1 expression in a time and dose-dependent manner. PMA treatment alone did not affect MCP-1 expression. Using chemical inhibitors of cell signaling pathways, we found that the NF-κB inhibitor inhibited TG-induced down-regulation of MCP-1. CCR2 expression decreased after TG treatment in THP-1 macrophages and the PKC inhibitor alleviated TG-induced down-regulation of CCR2. Our results provide further insights into the role of TG on macrophages during atherosclerosis.


Triglyceride Macrophage MCP-1 CCR2 Cell signaling Atherosclerosis 


Conflict of interest

The authors declare no conflicts of interest.


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Copyright information

© The Genetics Society of Korea 2013

Authors and Affiliations

  • Yoon Suk Kim
    • 1
  • Ho Joong Sung
    • 2
  • Sin Jee Son
    • 1
  • Jaewon Lim
    • 1
  • Yeo Wool Kang
    • 1
  • Tae Ue Kim
    • 1
  • Ki-Jong Rhee
    • 1
    Email author
  1. 1.Department of Biomedical Laboratory Science, College of Health SciencesYonsei UniversityGangwon-doRepublic of Korea
  2. 2.Department of Biomedical Laboratory Science, College of Health ScienceEulji UniversityGyeongi-DoRepublic of Korea

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