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Genes & Genomics

, Volume 35, Issue 2, pp 257–264 | Cite as

Molecular cytogenetic analysis of the Vigna species distributed in Korea

  • Hae-Woon Choi
  • Min-Young Kim
  • Sang-Hoon Lee
  • Sayeda Sultana
  • Jae-Wook Bang
Research Article

Abstract

Vigna plants distributed in Korea were analyzed by molecular cytogenetic fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and rDNA ITS/NTS sequences. FISH revealed that variable 45S rRNA gene loci (one to four) were localized on the terminal regions of chromosomes, while two conserved 5S rRNA gene loci from all species examined, except for rice bean (single locus), were detected. FISH and GISH showed the characteristic organization of rRNA gene loci and genomic homology on the chromosomes, indicating their cytogenetic relatationships. ITS sequence revealed that there was considerable variation in length (190–207 bp in ITS1, 205–221 bp in ITS2) and nucleotide composition (7–67 bp). The 5S rRNA gene unit comprised coding region (118 bp) and extensive sequence heterogeneity (97–221 bp). Phylogenetic analysis of the ITS and NTS sequences demonstrated that the Vigna species are divided into two groups: angularis (V. angularis, V. umbellata, V. nakashimae and V. nipponensis) and unguiculata (V. unguiculata, V. sesquipedalis and V. vexillata). Sequence data also showed that mung bean was closer to the angularis group.

Keywords

FISH GISH ITS/NTS rRNA gene Vigna 

Notes

Acknowledgments

This work was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Education, Science and Technology of Korea (NRF-20110025828).

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Copyright information

© The Genetics Society of Korea 2013

Authors and Affiliations

  1. 1.Department of Biological SciencesCollege of Biological Science and Biotechnology, Chungnam National UniversityDaejeonSouth Korea

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