A comparative, BAC end sequence enabled map of the genome of the American mink (Neovison vison)
- 290 Downloads
In this report we present the results of the analysis of approximately 2.7 Mb of genomic information for the American mink (Neovison vison) derived through BAC end sequencing. Our study, which encompasses approximately 1/1000th of the mink genome, suggests that simple sequence repeats (SSRs) are less common in the mink than in the human genome, whereas the average GC content of the mink genome is slightly higher than that of its human counterpart. The 2.7 Mb mink genomic dataset also contained 2,416 repeat elements (retroids and DNA transposons) occupying almost 31% of the sequence space. Among repeat elements, LINEs were over-represented and endogenous viruses (aka LTRs) under-represented in comparison to the human genome. Finally, we present a virtual map of the mink genome constructed with reference to the human and canine genome assemblies using a comparative genomics approach and incorporating over 200 mink BESs with unique hits to the human genome.
KeywordsAmerican mink BAC end sequencing Comparative mapping Structural genomics
Unable to display preview. Download preview PDF.
- Ansari S, Anistoroaei R, Farid A, Christensen K, and Benkel BF (2007) Characterization of microsatellite markers isolated from the American mink (Mustela vison) genome. Scientifur 31:55–58.Google Scholar
- Ewing B, Hillier L, Wendl MC, and Green P (1998) Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 8: 175–185.Google Scholar
- Farid A, Vincent I, Benkel B, and Christensen K (2004) Isolation of microsatellite markers for American mink (Mustela vison). Scientifur 28:228–233.Google Scholar
- Fur Commission USA (2007) Press release — November 2007, available at: www.furcommission.com/farming/pelts.htm.
- Ness N, Einarson E, Lohi O, and Jorgensen G (1988) Beautiful Fur Animals and Their Coat Colour Genetics. Scientifur, Glostrup, Denmark.Google Scholar
- Shackelford R (1948) The nature of coat color differences in mink and foxes. Genetics 33:311–336Google Scholar
- Stein N, Feuillet C, Wicker T, Schlagenhauf E, and Keller B (2000) Subgenome chromosome walking in wheat: A 450-kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat (Triticum aestivum L.). Proc. Natl. Acad. Sci. USA 97:13436–13441.PubMedCrossRefGoogle Scholar