Comparative genetic diversity of wild and hachery populations of Korean threadsail filefish Stephanolepis cirrhifer using cross-species microsatellite markers
- First Online:
- 313 Downloads
The threadsail filefish Stephanolepis cirrhifer is a highly commercial fisheries resource in Korea that suffers intensive anthropogenic pressure across much of its range. For basic information about its current genetic status in relation to stock enhancement, the level and distribution of genetic variation between a wild and a hatchery-bred population were investigated using 10 microsatellite markers developed for Thamnaconus modestus. High levels of polymorphism were observed between the two populations. A total of 95 different alleles were found at all loci, with some alleles being unique. The allelic variability ranged from six to 13 in the wild population and from five to 13 in the hatchery one. The average observed and expected heterozygosities were estimated to be 0.72 and 0.80 in the wild sample and 0.70 and 0.79 in the hatchery one, respectively. These results showed similar genetic variability in the hatchery population, as compared with the wild population and significant genetic differentiation between the wild population and the hatchery samples (FST = 0.016, P < 0.05). Genetic drift in the intensive breeding practices for stock enhancement has probably promoted differentiation between populations. Significant deviations from Hardy-Weinberg equilibrium were detected in both populations. Our results indicate that further studies using species-specific microsatellite markers will be necessary for a more reliable assessment of genetic diversity of the species.
KeywordsKorean filefish Stephanolepis cirrhifer Microsatellite loci Genetic diversity Heterozygosity
Unable to display preview. Download preview PDF.
- An HS, Kim MJ and Hong SW (2008) Genetic Diversity of Rock Bream Oplegnathus fasciatus in Southern Korea. Genes Genom.ics 30: 451–459.Google Scholar
- An HS, Lee JW, Dong CM, Noh JK and Myeong J-I (2011b) Development and characterization of microsatellite markers for genetic analysis of the Korean black scraper, Thamnaconus modestus. Genes Genom. accepted.Google Scholar
- Excoffier L, Laval G and Schneider S (2005) ARLEQUIN ver 3.0: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1, 47–50.Google Scholar
- Kim PD (2007) Scuba Diver. In Miniature guide to whole Korean fishes, P.D. Kim, eds., Poong Deung Press, Seoul, pp. 215.Google Scholar
- MIFAFF-Ministry for Food, Agriculture, Forestry and Fisheries of Korea (2009) Annual Statistics of Fisheries Production in 1990–2009. Ministry for Food, Agriculture, Forestry and Fisheries, Seoul (In Korean).Google Scholar