Genes & Genomics

, Volume 32, Issue 1, pp 47–53 | Cite as

Bottleneck in the endangered kalibaus, Labeo calbasu (cyprinidae: cypriniformes) populations in Bangladesh revealed by microsatellite DNA marker analysis

  • Debasish Saha
  • Md. Nahiduzzaman
  • Shirin Akter
  • Mohammad Nazrul Islam
  • Mostafa Ali Reza Hossain
  • Md. Samsul Alam
Research Article

Abstract

Microsatellite DNA marker analysis was carried out to assess the population genetic structure of an endangered carp, Labeo calbasu, collected from three different stocks; the Jamuna River, the Halda River and a Hatchery. Four heterologous microsatellite loci (Lr12, Lr14b, Lr21 and Lr24) identified from rohu (Labeo rohita) were analyzed to test the genetic variability of the target kalibaus stocks. The maximum number of alleles observed in loci Lr12, Lr14b, Lr21 and Lr24 were 10, 7, 8 and 6, respectively. The loci were found to be polymorphic (<P95) in all the populations. The average number of allele was highest in the Jamuna population (6.75) followed by that of the Halda (5.50) and the Hatchery population (4.25). The observed average heterozygosity (Ho) value was almost similar in all three populations. Except locus Lr12 in the Halda population, significant deviations from the Hardy-Weinberg Equilibrium were detected in all cases due to excess heterozygosity. The population differentiation values (FST) between all the population pairs were significant. The highest genetic distance value (D = 0.295) was measured between the Halda and the Hatchery populations. A recent bottleneck was observed in the Halda and the Hatchery population.

Keywords

Labeo calbasu Endangered species Genetic variation Microsatellite marker Bottleneck 

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Copyright information

© The Genetics Society of Korea and Springer Netherlands 2010

Authors and Affiliations

  • Debasish Saha
    • 1
  • Md. Nahiduzzaman
    • 1
  • Shirin Akter
    • 1
  • Mohammad Nazrul Islam
    • 2
  • Mostafa Ali Reza Hossain
    • 1
  • Md. Samsul Alam
    • 1
  1. 1.Department of Fisheries Biology and GeneticsBangladesh Agricultural UniversityMymensinghBangladesh
  2. 2.Department of BiotechnologyBangladesh Agricultural UniversityMymensinghBangladesh

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