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Genes & Genomics

, Volume 33, Issue 2, pp 187–195 | Cite as

Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L.) using microsatellite markers

  • Young-Il Cho
  • Jong-Hyun Park
  • Chul-Won Lee
  • Won-Hee Ra
  • Jong-Wook Chung
  • Jung-Ro Lee
  • Kyung-Ho Ma
  • Seok-Young Lee
  • Kang-Seob Lee
  • Myung-Chul Lee
  • Yong-Jin Park
Research Article

Abstract

Sixteen polymorphic microsatellite (SSR) markers, developed from an SSR-enriched genomic DNA library of sesame (Sesamum indicum L.), were used to assess genetic diversity, phylogenetic relationships, and population structure among 150 sesame accessions collected from 22 countries. A total of 121 alleles were detected among the sesame accessions. The number of detected alleles varied from 2 to 18, with an average of 7.6 alleles per locus. Polymorphism information content values ranged from 0.03 to 0.79, with an average of 0.42. These values indicated an excess of heterozygous individuals at 16 loci and an excess of homozygous individuals at three loci. Of these, 32 genotype-specific alleles were identified at 11 of 16 polymorphic SSR markers. Cluster analyses were performed by accession and population, revealing a complex accession distribution pattern with mean genetic similarity coefficient of 0.45 by accession and 0.52 by population. The wide variation in genetic similarity among the accessions revealed by SSRs reflected a high level of polymorphism at the DNA level. Model-based structure analysis revealed the presence of three groups that were basically consistent with the clustering results based on genetic distance. These findings may be used to augment the sesame germplasm and to increase the effectiveness of sesame breeding.

Keywords

Genetic diversity Population structure Sesame Sesamum indicum L. Simple sequence repeats 

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Copyright information

© The Genetics Society of Korea and Springer Netherlands 2011

Authors and Affiliations

  • Young-Il Cho
    • 1
  • Jong-Hyun Park
    • 2
  • Chul-Won Lee
    • 3
  • Won-Hee Ra
    • 1
  • Jong-Wook Chung
    • 1
    • 4
  • Jung-Ro Lee
    • 4
  • Kyung-Ho Ma
    • 4
  • Seok-Young Lee
    • 4
  • Kang-Seob Lee
    • 4
  • Myung-Chul Lee
    • 4
  • Yong-Jin Park
    • 1
    • 5
  1. 1.Department of Plant Resources, College of Industrial SciencesKongju National UniversityYesanKorea
  2. 2.Bio-resources Management Division, Research Policy BureauRural Development AdministrationSuwonKorea
  3. 3.Department of Crop ScienceChungbuk National UniversityCheongjuKorea
  4. 4.National Agrobiodiversity Center, National Institute of Agricultural BiotechnologyRural Development AdministrationSuwonKorea
  5. 5.Legume Bio-Resource Center of Green ManureKongju National UniversityYesanKorea

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