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Genes & Genomics

, Volume 32, Issue 3, pp 267–273 | Cite as

Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.)

  • Young-Il Cho
  • Jong-Wook Chung
  • Gi-An Lee
  • Kyung-Ho Ma
  • Anupam Dixit
  • Jae-Gyun Gwag
  • Yong-Jin ParkEmail author
Research Article

Abstract

Millets such as proso millet have excellent nutritional properties and could become a basic resource for crop breeding programs and food diversification. In this study, 25 polymorphic microsatellite markers were developed and characterized through construction of an SSR-enriched library from genomic DNA of proso millet (Panicum miliaceum L.). In total, 110 alleles were detected, with an average of 4.4 alleles per locus. Values of major allele frequency (M AF ) and expected heterozygosity (H E ) ranged from 0.36 to 0.98 (mean = 0.73) and from 0.04 to 0.74 (mean = 0.37), respectively. The mean genetic similarity coefficient was 0.3711, indicating that among 50 accessions of proso millet there was wide genetic variation. The newly developed microsatellite markers should be useful tools for assessing genetic diversity, understanding population structure, and breeding of proso millet.

Keywords

Microsatellite markers Panicum miliaceum L. Proso millet SSR-enriched library 

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Copyright information

© The Genetics Society of Korea and Springer Netherlands 2010

Authors and Affiliations

  • Young-Il Cho
    • 1
  • Jong-Wook Chung
    • 1
  • Gi-An Lee
    • 2
  • Kyung-Ho Ma
    • 2
  • Anupam Dixit
    • 3
  • Jae-Gyun Gwag
    • 2
  • Yong-Jin Park
    • 1
    Email author
  1. 1.Department of Plant Resources, College of Industrial ScienceKongju National UniversityYesanKorea
  2. 2.National Agrobiodiversity Center, National Institute of Agricultural BiotechnologyRDASuwonKorea
  3. 3.Rice DNA and Quality Testing Laboratory, Basmati Export Development FoundationSVBP University of Agriculture and TechnologyMeerutIndia

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