Fourier Transform Infrared Spectroscopic Imaging-Derived Collagen Content and Maturity Correlates with Stress in the Aortic Wall of Abdominal Aortic Aneurysm Patients

  • Rabee Cheheltani
  • Joseph E. Pichamuthu
  • Jayashree Rao
  • Justin S. Weinbaum
  • Mohammad F. Kiani
  • David A. Vorp
  • Nancy Pleshko
Article

Abstract

Abdominal aortic aneurysm (AAA) is a degenerative disease of the aorta characterized by severe disruption of the structural integrity of the aortic wall and its major molecular constituents. From the early stages of disease, elastin in the aorta becomes highly degraded and is replaced by collagen. Questions persist as to the contribution of collagen content, quality and maturity to the potential for rupture. Here, using our recently developed Fourier transform infrared imaging spectroscopy (FT-IRIS) method, we quantified collagen content and maturity in the wall of AAA tissues in pairs of specimens with different wall stresses. CT scans of AAAs from 12 patients were used to create finite element models to estimate stress in different regions of tissue. Each patient underwent elective repair of the AAA, and two segments of the AAA tissues from anatomic regions more proximal or distal with different wall stresses were evaluated by histology and FT-IRIS after excision. For each patient, collagen content was generally greater in the tissue location with lower wall stress, which corresponded to the more distal anatomic regions. The wall stress/collagen ratio was greater in the higher stress region compared to the lower stress region (1.01 ± 1.09 vs. 0.55 ± 0.084, p = 0.02). The higher stress region also corresponded to the location with reduced intraluminal thrombus thickness. Further, collagen maturity tended to decrease with increased collagen content (p = 0.068, R = 0.38). Together, these results suggest that an increase in less mature collagen content in AAA patients does not effectively compensate for the loss of elastin in the aortic wall, and results in a reduced capability to endure wall stresses.

Keywords

Aneurysm Collagen Stress FTIR Extracellular matrix 

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Copyright information

© Biomedical Engineering Society 2016

Authors and Affiliations

  • Rabee Cheheltani
    • 1
    • 6
  • Joseph E. Pichamuthu
    • 2
  • Jayashree Rao
    • 2
  • Justin S. Weinbaum
    • 2
    • 3
  • Mohammad F. Kiani
    • 1
  • David A. Vorp
    • 2
    • 3
    • 4
  • Nancy Pleshko
    • 5
  1. 1.Department of Mechanical EngineeringTemple UniversityPhiladelphiaUSA
  2. 2.Department of BioengineeringUniversity of PittsburghPittsburghUSA
  3. 3.McGowan Institute for Regenerative Medicine, University of PittsburghPittsburghUSA
  4. 4.Department of Surgery, Department of Cardiothoracic Surgery, and Center for Vascular Remodeling and RegenerationUniversity of PittsburghPittsburghUSA
  5. 5.Department of BioengineeringTemple UniversityPhiladelphiaUSA
  6. 6.Department of RadiologyUniversity of PennsylvaniaPhiladelphiaUSA

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