Advertisement

Correction to: Gene activation in human cells using CRISPR/Cpf1-p300 and CRISPR/Cpf1-SunTag systems

  • Xin Zhang
  • Wei Wang
  • Lin Shan
  • Le Han
  • Shufeng Ma
  • Yan Zhang
  • Bingtao Hao
  • Ying LinEmail author
  • Zhili RongEmail author
Open Access
Correction
  • 297 Downloads

Correction to: Protein Cell 2018, 9(4):380–383  https://doi.org/10.1007/s13238-017-0491-6

In the original publication the Supplementary Material and Fig. 2 are incorrect. The correct version of Supplementary Material and Fig. 2 are provided in this correction article. The text HBG2 appearing in the article should be read as HBG1.
Figure 2

Simultaneously transcriptional activation of multiple endogenous genes using either dLbCpf1-p300core or dLbCpf1-SunTag system with a single gRNA for each gene. (A) Relative mRNA expression of MYOD, IL1RN, and HBG1 revealed by quantitative real-time PCR, in HEK293Tcells co-transfected with dCpf1-p300core fusion proteins and four single gRNAs or pooled sets of all four single gRNAs targeting each promoter region of target genes. (B) Relative mRNA expression of HBG1 revealed by quantitative RT-PCR, in HEK293T cells co-transfected with dCpf1-p300core fusion proteins and four single gRNAs or pooled sets of all four single gRNAs targeting the enhancer region (HS2 region) of HBG1 gene. (C) Relative mRNA expression of MYOD, HBG1, and IL1RN revealed by quantitative RT-PCR, in HEK293Tcells co-transfected with dCpf1-p300core fusion proteins and three gRNAs targeting each promoter region of target genes. (D) Relative mRNA expression of MYOD, HBG1, and IL1RN revealed by quantitative RT-PCR, in HEK293T cells co-transfected with dLbCpf1 (M925)-SunTag and three gRNAs targeting each promoter region of target genes. For C and D, gRNA1, gRNA2 and gRNA1 were used for MYOD, HBG1 and IL1RN, respectively. For (A–D), mean value are presented with S.D. (n = 3). Tukey-test, P < 0.05 compared to cells transfected with dCpf1-p300core or dLbCpf1(M925)-SunTag only, n = 3 independent experiments

Supplementary material

13238_2018_585_MOESM1_ESM.pdf (676 kb)
Supplementary material 1 (PDF 677 kb)

Copyright information

© HEP and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Cancer Research Institute, School of Basic Medical SciencesSouthern Medical UniversityGuangzhouChina

Personalised recommendations