Advertisement

Correction to: Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg−/− mice

  • Yaqin Du
  • Ting Wang
  • Jun Xu
  • Chaoran Zhao
  • Haibo Li
  • Yao Fu
  • Yaxing Xu
  • Liangfu Xie
  • Jingru Zhao
  • Weifeng Yang
  • Ming Yin
  • Jinhua Wen
  • Hongkui Deng
Open Access
Correction
  • 301 Downloads

Correction to: Protein Cell  https://doi.org/10.1007/s13238-018-0558-z

In the original publication Fig. 1D and supplementary material is incorrect. The correct Fig. 1D and supplementary material is provided in this correction.
Figure 1

Generation of NOD-scid Il2rg−/− extended pluripotent stem cells. (A) Schematic of two approaches used for generating NOD-scid Il2rg−/− extended pluripotent stem cells: de novo derivation from blastocysts (upper panels) and chemical reprogramming from embryonic fibroblasts (lower panels). (B) Phase-contrast images of de novo derived outgrowth and EPS colonies for 17 passages in LCDM medium. Scale bars, 100 μm. (C) qRT-PCR analysis of XEN marker genes expression during the chemical induction process (day 16). Error bars indicate SEM (n = 2). (D) Co-immunostaining of XEN marker genes during the chemical induction process (day 16). Upper panels: GATA6 and SALL4; lower panels: SOX17 and SALL4. Scale bars, 100 μm. (E) Immunofluorescence of OCT4-positive primary colonies at the end of the chemical induction (day 40). Scale bars, 100 μm. (F) Typical karyotypes of EPS (passage 22) and cEPS (passage 23) cells. Each cell line counts 30 cells

Supplementary material

13238_2018_571_MOESM1_ESM.pdf (170 kb)
Electronic supplementary material 1 (PDF 170 kb)
13238_2018_571_MOESM2_ESM.pptx (62 kb)
Electronic supplementary material 2 (PPTX 62 kb)

Copyright information

© HEP and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Yaqin Du
    • 1
  • Ting Wang
    • 1
  • Jun Xu
    • 1
  • Chaoran Zhao
    • 1
  • Haibo Li
    • 1
  • Yao Fu
    • 3
  • Yaxing Xu
    • 2
  • Liangfu Xie
    • 3
  • Jingru Zhao
    • 2
  • Weifeng Yang
    • 4
  • Ming Yin
    • 4
  • Jinhua Wen
    • 1
  • Hongkui Deng
    • 1
    • 2
    • 3
  1. 1.Peking University Stem Cell Research Center, Department of Cell Biology, School of Basic Medical SciencesPeking University Health Science CenterBeijingChina
  2. 2.Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, College of Life SciencesPeking UniversityBeijingChina
  3. 3.The MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life SciencesPeking UniversityBeijingChina
  4. 4.Beijing Vitalstar BiotechnologyBeijingChina

Personalised recommendations