Protein & Cell

, Volume 2, Issue 6, pp 487–496 | Cite as

Interactomic study on interaction between lipid droplets and mitochondria

  • Jing Pu
  • Cheol Woong Ha
  • Shuyan Zhang
  • Jong Pil Jung
  • Won-Ki HuhEmail author
  • Pingsheng LiuEmail author
Research Article


An increasing body of evidence shows that the lipid droplet, a neutral lipid storage organelle, plays a role in lipid metabolism and energy homeostasis through its interaction with mitochondria. However, the cellular functions and molecular mechanisms of the interaction remain ambiguous. Here we present data from transmission electron microscopy, fluorescence imaging, and reconstitution assays, demonstrating that lipid droplets physically contact mitochondria in vivo and in vitro. Using a bimolecular fluorescence complementation assay in Saccharomyces cerevisiae, we generated an interactomic map of protein-protein contacts of lipid droplets with mitochondria and peroxisomes. The lipid droplet proteins Erg6 and Pet10 were found to be involved in 75% of the interactions detected. Interestingly, interactions between 3 pairs of lipid metabolic enzymes were detected. Collectively, these data demonstrate that lipid droplets make physical contacts with mitochondria and peroxisomes, and reveal specific molecular interactions that suggest active participation of lipid droplets in lipid metabolism in yeast.


peroxisomes bimolecular fluorescence complementation assay protein-protein interaction lipid metabolism Erg6 

Supplementary material

13238_2011_1061_MOESM1_ESM.pdf (258 kb)
Supplementary material, approximately 257 KB.


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Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  1. 1.National Laboratory of Biomacromolecules, Institute of BiophysicsChinese Academy of SciencesBeijingChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina
  3. 3.School of Biological Sciences, Research Center for Functional Cellulomics, Institute of MicrobiologySeoul National UniversitySeoulRepublic of Korea

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