Protein & Cell

, Volume 1, Issue 12, pp 1106–1117 | Cite as

Mechanism of inhibiting type I interferon induction by hepatitis B virus X protein

  • Junyi Jiang
  • Hong TangEmail author
Research Article


Hepatitis B virus (HBV) is regarded as a stealth virus, invading and replicating efficiently in human liver undetected by host innate antiviral immunity. Here, we show that type I interferon (IFN) induction but not its downstream signaling is blocked by HBV replication in HepG2.2.15 cells. This effect may be partially due to HBV X protein (HBx), which impairs IFNβ promoter activation by both Sendai virus (SeV) and components implicated in signaling by viral sensors. As a deubiquitinating enzyme (DUB), HBx cleaves Lys63-linked polyubiquitin chains from many proteins except TANK-binding kinase 1 (TBK1). It binds and deconjugates retinoic acid-inducible gene I (RIG I) and TNF receptor-associated factor 3 (TRAF3), causing their dissociation from the downstream adaptor CARDIF or TBK1 kinase. In addition to RIG I and TRAF3, HBx also interacts with CARDIF, TRIF, NEMO, TBK1, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKKi) and interferon regulatory factor 3 (IRF3). Our data indicate that multiple points of signaling pathways can be targeted by HBx to negatively regulate production of type I IFN.


hepatitis B virus (HBV) HBV X protein (HBx) deubiquitination type I interferon 

Supplementary material

13238_2010_141_MOESM1_ESM.pdf (414 kb)
Supplementary material, approximately 340 KB.


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Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Key laboratory of Infection and Immunity of Chinese Academy of SciencesInstitute of BiophysicsBeijingChina
  2. 2.Graduate School of Chinese Academy of SciencesBeijingChina

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