Engineering of a genome-reduced host: practical application of synthetic biology in the overproduction of desired secondary metabolites
- 267 Downloads
Synthetic biology aims to design and build new biological systems with desirable properties, providing the foundation for the biosynthesis of secondary metabolites. The most prominent representation of synthetic biology has been used in microbial engineering by recombinant DNA technology. However, there are advantages of using a deleted host, and therefore an increasing number of biotechnology studies follow similar strategies to dissect cellular networks and construct genomereduced microbes. This review will give an overview of the strategies used for constructing and engineering reduced-genome factories by synthetic biology to improve production of secondary metabolites.
Keywordssynthetic biology reduced-genome secondary metabolite
- Debabov, V.G. (2003). The threonine story. Adv Biochem Eng Biotechnol 79, 113–136.Google Scholar
- Gao, H., Zhou, X., Gou, Z., Zhuo, Y., Fu, C., Liu, M., Song, F., Ashforth, E., and Zhang, L. (2010). Rational design for overproduction of desirable microbial metabolites by precision engineering. Antonie van Leeuwenhoek. In press.Google Scholar
- Murakami, K., Tao, E., Ito, Y., Sugiyama, M., Kaneko, Y., Harashima, S., Sumiya, T., Nakamura, A., and Nishizawa, M. (2007). Large scale deletions in the Saccharomyces cerevisiae genome create strains with altered regulation of carbon metabolism. Appl Microbiol Biotechnol 75, 589–597.CrossRefGoogle Scholar
- Zhuo, Y., Zhang, W., Chen, D., Gao, H., Tao, J., Liu, M., Gou, Z., Zhou, X., BC, Y., Zhang, Q., et al. (2010). Reverse biological engineering of hrdB to enhance the production of avermectins in an industrial strain of Streptomyces avermitilis. Proc Natl Acad Sci U S A. In press.Google Scholar