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The Nucleus

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Selenium nanoparticles are less toxic than inorganic and organic selenium to mice in vivo

  • Arin Bhattacharjee
  • Abhishek Basu
  • Sudin BhattacharyaEmail author
Original Article
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Abstract

Nanoparticles (NPs) provide versatile means to reduce the toxicity, enhance bioactivity and improve targeting of cells. The antioxidant and pro-oxidant effects, or bioavailability and toxicity, of selenium depend on its chemical form. In the present study the effects of nano-selenium (Nano-Se) was compared with inorganic and organic selenium on the basis of their antioxidative activities and hematological parameters in Swiss albino mice. At an oral dose of 2 mg Se/kg b.w. per day administered for consecutive 28 days, both forms of selenium suppressed mice growth rather than Nano-Se. Abnormal liver and kidney function were more pronounced with selenite treatment than Nano-Se as indicated by the increase of hepatotoxic and renal toxic marker in serum and also confirmed by histological examination. After being treated with different forms of selenium it can be seen that the activity of enzymes have increased considerably in case of Nano-Se. Synthesized selenium nanoparticles, caused less bone marrow cell death and prevented DNA damage, compared to other forms of selenium. Our results suggest that Nano-Se as an antioxidant can serve as a potential chemopreventive agent with reduced risk of selenium toxicity.

Keywords

Nanoparticle of selenium (Nano-Se) Oxidative stress Toxicity Phase II detoxifying enzymes Comet assay 

Notes

Acknowledgements

Arin Bhattacharjee gratefully acknowledges Indian Council of Medical Research (ICMR) for Senior Research Fellowship (No. 45/36/2008/PHA-BMS). Abhishek Basu also gratefully acknowledges ICMR for Senior Research Fellowship (No. 3/2/2/58/2011/NCD-III). The authors wish to thank the Director, CNCI, for supporting this study.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

References

  1. 1.
    Ahmadinejad F, Geir Møller S, Hashemzadeh-Chaleshtori M, Bidkhori G, Jami MS. Molecular mechanisms behind free radical scavengers function against oxidative stress. Antioxidants (Basel). 2017;6(3):51.CrossRefGoogle Scholar
  2. 2.
    ATSDR. Toxicological profiles for selenium. Atlanta: U.S. Department of Health and Human Services, Public Health Service, Agency for Toxic Substances and Disease Registry; 2003. p. 1–457.Google Scholar
  3. 3.
    Balogh K, Elbaraasi H, Mezes M. Selenium toxicity in fishes. Halaszat. 2002;95:30–3 (in Hungarian).Google Scholar
  4. 4.
    Basu A, Bhattacharjee A, Samanta A, Bhattacharya S. Prevention of cyclophosphamide-induced hepatotoxicity and genotoxicity: effect of an l-cysteine based oxovanadium(IV) complex on oxidative stress and DNA damage. Environ Toxicol Pharmacol. 2015;40:747–57.CrossRefPubMedGoogle Scholar
  5. 5.
    Basu A, Singha Roy S, Bhattacharjee A, Bhuniya A, Baral R, Biswas J, Bhattacharya S. Vanadium(III)-l-cysteine protects cisplatin-induced nephropathy through activation of Nrf2/HO-1 pathway. Free Radic Res. 2016;50(1):39–55.CrossRefPubMedGoogle Scholar
  6. 6.
    Bhattacharjee A, Basu A, Biswas J, Bhattacharya S. Nano-Se attenuates cyclophosphamide-induced pulmonary injury through modulation of oxidative stress and DNA damage in Swiss albino mice. Mol Cell Biochem. 2015;405:243–56.CrossRefPubMedGoogle Scholar
  7. 7.
    Bhattacharjee A, Basu A, Ghosh P, Biswas J, Bhattacharya S. Protective effect of selenium nanoparticle against cyclophosphamide induced hepatotoxicity and genotoxicity in Swiss albino mice. J Biomater Appl. 2014;29:303–17.CrossRefPubMedGoogle Scholar
  8. 8.
    Birge WJ. Aquatic toxicology of trace elements of coal and fly ash. In: Thorp JH, Gibbons JW, editors. Energy and environmental stress in aquatic systems, vol. 48. Washington: Department of Energy Symposium Series; 1978. p. 219–40.Google Scholar
  9. 9.
    Biswas SJ, Pathak S, Khuda Bukhsh AR. Assessment of the genotoxic and cytotoxic potential of an antiepileptic drug phenobarbital, in mice: a time course study. Mutat Res. 2004;563:1–11.CrossRefPubMedGoogle Scholar
  10. 10.
    Carl Allinson MJ. A specific enzymatic method for the determination of creatine and creatinine in blood. J Biol Chem. 1945;157:169–72.Google Scholar
  11. 11.
    Chaudiere J, Courtin O, Leclaire J. Glutathione oxidase activity of selenocystamine: a mechanistic study. Arch Biochem Biophys. 1992;296:328–36.CrossRefPubMedGoogle Scholar
  12. 12.
    D’Armour FE, Blood FR, Belden DA. The manual for laboratory work in mammalian physiology. 3rd ed. Chicago: The University of Chicago Press; 1965.Google Scholar
  13. 13.
    Fernandes AP, Gandin V. Selenium compounds as therapeutic agents in cancer. Biochim Biophys Acta. 2015;1850:1642–60.CrossRefPubMedGoogle Scholar
  14. 14.
    Ganther HE. Metabolism of hydrogen selenide and methylated selenides. Adv Nutr Res. 1979;2:107–28.CrossRefGoogle Scholar
  15. 15.
    Goehring TB. Toxic effects of selenium on growing swine fed corn–soybean meal diets. J Anim Sci. 1984;59:733–7.CrossRefPubMedGoogle Scholar
  16. 16.
    Green DE, Albers PH. Diagnostic criteria for selenium toxicosis in aquatic birds: histologic lesions. J Wildl Dis. 1997;33:385–404.CrossRefPubMedGoogle Scholar
  17. 17.
    Habig WH, Pabst MJ, Jacoby WB. Glutathione S-transferases, the first enzymatic step in marcapturic acid formation. J Biol Chem. 1974;249:7130–9.PubMedGoogle Scholar
  18. 18.
    Halliwell RE. Autoimmune diseases in domestic animals. J Am Vet Med Assoc. 1982;18:1088–96.Google Scholar
  19. 19.
    Hilton JW, Hodson PV, Slinger SJ. Absorption, distribution, half-life and possible routes of elimination of dietary selenium in juvenile rainbow trout (Salmo gairdneri). Comp Biochem Physiol. 1982;71C:49–55.Google Scholar
  20. 20.
    Hosnedlova B, Kepinska M, Skalickova S, Fernandez C, Ruttkay-Nedecky B, Peng Q. Nano-selenium and its nanomedicine applications: a critical review. Int J Nanomed. 2018;13:2107–28.CrossRefGoogle Scholar
  21. 21.
    Hurst R, Collings R, Harvey LJ, King M, Hooper L, Bouwman J, Gurinovic M, Fairweather-Tait SJ. EURRECA—estimating selenium requirements for deriving dietary reference values. Crit Rev Food Sci Nutr. 2013;53:1077–96.CrossRefPubMedGoogle Scholar
  22. 22.
    Ibrahim SAZ, Kerkadi A, Agouni A. Selenium and health: an update on the situation in the Middle East and North Africa. Nutrients. 2019;11(7):1457.CrossRefPubMedCentralGoogle Scholar
  23. 23.
    Kind PR, King EJ. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol. 1954;7:322–6.CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Kumar S, Tomar MS, Acharya A. Carboxylic group-induced synthesis and characterization of selenium nanoparticles and its anti-tumor potential on Dalton’s lymphoma cells. Colloids Surf B Biointerfaces. 2015;126:546–52.CrossRefPubMedGoogle Scholar
  25. 25.
    Kuria A, Fang X, Li M, Han H, He J, Aaseth JO, Cao Y. Does dietary intake of selenium protect against cancer? A systematic review and meta-analysis of population-based prospective studies. Crit Rev Food Sci Nutr. 2018;20:1–11.CrossRefGoogle Scholar
  26. 26.
    Leme DM, Marin-Morales MA. Chromosome aberration and micronucleus frequencies in Allium cepa cells exposed to petroleum polluted water—a case study. Mutat Res. 2008;650:80.CrossRefPubMedGoogle Scholar
  27. 27.
    Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem. 1951;193:265–76.PubMedPubMedCentralGoogle Scholar
  28. 28.
    Lubos E, Loscalzo J, Handy DE. Glutathione peroxidase-1 in health and disease: from molecular mechanisms to therapeutic opportunities. Antioxid Redox Signal. 2011;15:1957–97.CrossRefPubMedPubMedCentralGoogle Scholar
  29. 29.
    Luck HA. Spectrophotometric method for estimation of catalase. In: Bergmeyer HV, editor. Methods of enzymatic analysis. New York: Academic Press; 1963. p. 886–8.Google Scholar
  30. 30.
    Marklund S, Marklund G. Involvement of the superoxide anion radical in autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47:469–74.CrossRefPubMedGoogle Scholar
  31. 31.
    Mather A, Roland D. The automated thiosemicarbazide-diacetyl monoxime method for plasma urea. Clin Chem. 1969;15:393–6.PubMedGoogle Scholar
  32. 32.
    McCord JM, Fridovich I. Superoxide dismutase: an enzymatic function for erythrocuprein (hemoprotein). J Biol Chem. 1969;244:6049–55.PubMedGoogle Scholar
  33. 33.
    McDowell LR. Minerals for grazing ruminants in tropical regions. Gainesville: Bull Univ Florida; 1997. p. 1–69.Google Scholar
  34. 34.
    Moeasgaard S, Morrill R. The need for speciation to realise the potential of selenium in disease prevention. In: Ebdon L, editor. Trace element speciation for environment, food and health. London: Royal Society of Chemistry; 2002. p. 261–83.Google Scholar
  35. 35.
    Mousa SA, Bharali DJ. Nanotechnology-based detection and targeted therapy in cancer: nano-bio paradigms and applications. Cancers (Basel). 2011;3:2888–903.CrossRefPubMedPubMedCentralGoogle Scholar
  36. 36.
    Mullur R, Liu YY, Brent GA. Thyroid hormone regulation of metabolism. Physiol Rev. 2014;94:355–82.CrossRefPubMedPubMedCentralGoogle Scholar
  37. 37.
    Mustacich D, Powis G. Thioredoxin reductase. Biochem J. 2000;346:1–8.CrossRefPubMedPubMedCentralGoogle Scholar
  38. 38.
    Øarskov H, Flyvbjerg A. Selenium and human health. Lancet. 2000;356:942–3.CrossRefGoogle Scholar
  39. 39.
    Okhawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Ann Biochem. 1979;95:351–8.CrossRefGoogle Scholar
  40. 40.
    Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70:158–69.PubMedGoogle Scholar
  41. 41.
    Poole LG, Dolin CE, Arteel GE. Organ–organ crosstalk and alcoholic liver disease. Biomolecules. 2017;7:3.CrossRefGoogle Scholar
  42. 42.
    Rayman MP. Selenium and human health. Lancet. 2012;379:1256–68.CrossRefPubMedGoogle Scholar
  43. 43.
    Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957;28:56–63.CrossRefPubMedGoogle Scholar
  44. 44.
    Ren X, Zou L, Zhang X, Branco V. Redox signaling mediated by thioredoxin and glutathione systems in the central nervous system. Antioxid Redox Signal. 2017;27:989–1010.CrossRefPubMedPubMedCentralGoogle Scholar
  45. 45.
    Rodríguez-Hernández Á, Zumbado M, Henríquez-Hernández LA, Boada LD, Luzardo OP. Dietary intake of essential, toxic, and potentially toxic elements from mussels (Mytilus spp.) in the Spanish population: a nutritional assessment. Nutrients. 2019;17(4):864.CrossRefGoogle Scholar
  46. 46.
    Sahil H. Klinische Untersuchungsmethoden. 5th ed. Wien: Leipsic and Vienna; 1909. p. 845.Google Scholar
  47. 47.
    Sedlack J, Lindsay RN. Estimation of total protein bound and non-protein sulfhydryl groups in tissue with Ellman’s reagent. Ann Biochem. 1968;25:192–205.CrossRefGoogle Scholar
  48. 48.
    Seko Y, Saito Y, Kitahara J, Imura N. Active oxygen generation by the reaction of selenite with reduced glutathione in vitro. In: Wendel A, editor. Selenium in biology and medicine. Berlin: Springer; 1989. p. 70–3.CrossRefGoogle Scholar
  49. 49.
    Singh NP, McCoy MT, Tice RR. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988;175:184–91.CrossRefPubMedGoogle Scholar
  50. 50.
    Snider GW, Ruggles E, Khan N, Hondal RJ. Selenocysteine confers resistance to inactivation by oxidation in thioredoxin reductase: comparison of selenium and sulfur enzymes. Biochemistry. 2013;52:5472–81.CrossRefPubMedPubMedCentralGoogle Scholar
  51. 51.
    Stoffaneller R, Morse NL. A review of dietary selenium intake and selenium status in Europe and the Middle East. Nutrients. 2015;7:1494–537.CrossRefPubMedPubMedCentralGoogle Scholar
  52. 52.
    Thorlacius-Ussing O. Selenium-induced growth retardation. Histochemical and endocrinological studies on the anterior pituitaries of selenium treated rats. Dan Med Bull. 1990;37(4):347–58.PubMedGoogle Scholar
  53. 53.
    Ungvári É, Monori I, Megyeri A, Csiki Z, Prokisch J, Sztrik A, Jávor A, Benkő I. Protective effects of meat from lambs on selenium nanoparticle supplemented diet in a mouse model of polycyclic aromatic hydrocarbon-induced immunotoxicity. Food Chem Toxicol. 2014;64:298–306.CrossRefPubMedGoogle Scholar
  54. 54.
    Us EPA. Ambient water quality criteria for selenium—EPA-440/5-87-006. Washington: U.S. Environmental Protection Agency, Office of Water Regulation and Standards; 1987. p. 1–23.Google Scholar
  55. 55.
    Valdiglesias V, Pásaro E, Méndez J, Laffon B. In vitro evaluation of selenium genotoxic, cytotoxic, and protective effects: a review. Arch Toxicol. 2010;84:337–51.CrossRefPubMedGoogle Scholar
  56. 56.
    Wang X, Zhang J, Xu T. Cyclophosphamide as a potent inhibitor of tumor thioredoxin reductase in vivo. Toxicol Appl Pharmacol. 2007;218:88–95.CrossRefPubMedGoogle Scholar
  57. 57.
    Wintrobe MM, Lee DR, Boggs DR, Bithel TC, Athens JW, Foerester J. Clinical hematology. 5th ed. Philadelphia: Les and Febiger; 1961.Google Scholar
  58. 58.
    Xie ZZ, Liu Y, Bian JS. Hydrogen sulfide and cellular redox homeostasis. Oxid Med Cell Longev. 2016;2016:6043038.CrossRefPubMedPubMedCentralGoogle Scholar
  59. 59.
    Yang G, Wang S, Zhou R, Sun S. Endemic selenium intoxication of humans in China. Am J Clin Nutr. 1982;37:872–5.CrossRefGoogle Scholar
  60. 60.
    Zhang J, Wang H, Bao Y, Zhang L. Nano red elemental selenium has no size effect in the induction of seleno-enzymes in both cultured cells and mice. Life Sci. 2004;75:237–44.CrossRefPubMedGoogle Scholar

Copyright information

© Archana Sharma Foundation of Calcutta 2019

Authors and Affiliations

  • Arin Bhattacharjee
    • 1
    • 2
  • Abhishek Basu
    • 1
    • 3
  • Sudin Bhattacharya
    • 1
    • 4
    Email author
  1. 1.Chittaranjan National Cancer InstituteKolkataIndia
  2. 2.Global College of Pharmaceutical TechnologyNadiaIndia
  3. 3.BengaluruIndia
  4. 4.South 24 ParganasIndia

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