Karyotype analysis and report on B-chromosome in Gloriosa superba L. by differential staining
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In this investigation Gloriosa superba L. was cytogenetically studied with orcein, CMA and DAPI-staining for authentic characterization. “Complex Chromocenter Type” of interphase nuclei with 3–4 bigger darkly stained heterochromatic regions was found in this species. The prophase chromosomes were “Interstitial Type” with darkly stained region at different interstitial sites of chromosomes. This species had 2n = 22 chromosomes with heterogenous centromeric formulae 14 m + 8 sm. In addition to the regular chromosomes, 1–6 small chromosomes were found in several mitotic pro-metaphase, metaphase and anaphase stage which covered 43.57% of the total cell count of this species suggesting the probable occurrence of B-chromosome. Presence of B-chromosome was probably the first report for this genus. After fluorescent banding, a pair of dot like CMA fluoresced bands were observed whereas no bright band was found in G. superba after DAPI-staining which suggested the coexistence of GC- and AT-base pairs in the genome. Thus, the compilation of the above information will be very useful for cytogenetical characterization of G. superba L. in Bangladesh.
KeywordsKaryotype CMA DAPI B-chromosome Bimodality Gloriosa superba
This research work was funded by Bangabandhu Science and Technology Fellowship Trust, Ministry of Science and Technology, Government of the Peoples’ Republic of Bangladesh.
SSS—Field investigation, performing the experiment, data analysis, drafting manuscript; CKD—Material collection, performing the experiment, data analysis; SSA—Study design, originator of the research, interpretation of results; MAH—Study design, interpretation of results, critical revision and finalization of the manuscript.
- 5.Ali M, Moghaddam B, Schubert V, Kumke K, Weiβ O, Klemme S, Nagaki K, Macas J, González-Sánchez M, Heredia V, Gómez-Revilla D, González-García M, Vega JM, Puertas MJ, Houben A. Nondisjunction in favor of a chromosome: the mechanism of rye B chromosome drive during pollen mitosis. Plant Cell. 2012;24:4124–34.CrossRefGoogle Scholar
- 7.Biswas A, Muntaha SN, Rahman MM. Comparative karyotype analysis in two life-forms of Gloriosa superba L. J Pharma Biol. 2014;4:77–80.Google Scholar
- 8.Bose TK, Yadab LP. Commercial flowers. Naya Prakash. 1989.Google Scholar
- 10.Delay C. Recherches sur la structure des noyaux quiescents chez les Phanerogames. Rev. Cytol. et Cytophysiol Veg. 1947;9:169–222.Google Scholar
- 17.Khan MS. Towards sustainable development: conservation of genetic resources of Bangladesh, a background paper for national conservation strategies-Bangladesh. The World Conservation Union and Bangladesh Agricultural Research Council; 1995.Google Scholar
- 18.Khoshoo TN. Cytology of Gloriosa. Curr Sci. 1956;25:165–6.Google Scholar
- 19.Komissarov A, Vij S, Yurchenko A, Trifonov V, Thevasagayam N, Saju J, Sridatta PSR, Purushothaman K, Graphodatsky A, Orbán L, Kuznetsova I. B chromosomes of the Asian seabass (Lates calcarifer) contribute to genome variations at the level of individuals and populations. Genes. 2018;9:464.CrossRefGoogle Scholar
- 20.Kondo T, Hizume M. Banding for the chromosomes of Cryptomeria japonica D. Don Jpn J For Soc. 1982;64:356–8.Google Scholar
- 24.Maroyi A, Maesen LJGVD. Gloriosa superba L. (family Colchicaceae): Remedy or poison? J. Med Pl Res. 2011;5:6112–21.Google Scholar
- 26.Miller EW. A preliminary note on the cytology of the Melanthioideae section of the Liliaceae. Proc Univ Durham Philos Soc. 1930;8:267–71.Google Scholar
- 27.Mitra K, Datta N. IOPB chromosome number reports VIII. Taxon. 1967;16:445–61.Google Scholar
- 30.Padmapriya S, Rajamani K, Sathiyamurthy VA. Glory Lily (Gloriosa superba L.)—a review. Int J Curr Pharm Rev Res. 2015;7:43–9.Google Scholar
- 31.Ragupathi G. Phytochemical and antioxidant screening of Gloriosa superba L. from different geographical positions of South India. Int J Bot Stud. 2016;1:13–9.Google Scholar
- 32.Sato D. Karyotype alteration and phylogeny in Liliaceae and allied families. Jpn J Bot. 1942;12:57–161.Google Scholar
- 35.Sharma AK, Sharma A. An investigation of the cytology of some species of Liliaceae. Genet Iber. 1961;13:25–42.Google Scholar
- 36.Siddiqui KU, Islam MA, Ahmed ZU, Begum ZNT, Hassan MA, Khondker M, Rahman MM, Kabir SMH, Ahmad M, Ahmed ATM, Rahman AKA, Haque EU. Encyclopedia of flora and fauna of Bangladesh. Angiosperms: Monocotyledons (Agavaceae-Najadaceae), Asiat Soc Bangladesh; 2007.Google Scholar
- 37.Stebbins GL. Chromosomal evolution in higher plants. Boston: Addison-Wesley publishing company; 1971.Google Scholar