The Nucleus

, Volume 60, Issue 1, pp 25–31 | Cite as

The genetic differentiation of Nile Tilapia, Oreochromis niloticus, is affected by chromium and lead in stabilization pond of municipal wastewater treatment

  • Bundit Tengjaroenkul
  • Weerayuth Supiwong
  • Pansa Monkheang
  • Rattikran Udomphan
  • Sirilak Boonmee
  • Sutee Chowrong
  • Lamyai Neeratanaphan
Original Article
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Abstract

This study aims to determine the heavy metals concentrations in the Nile tilapia, Oreochromis niloticus, correlates with genetic differentiation in a fish cultured farm and a stabilization pond. The heavy metals including cadmium (Cd), chromium (Cr) and lead (Pb) concentrations were analyzed using induction coupled plasma-mass spectrometry. Genetic differentiation was studied by random amplified polymorphic DNA (RAPD) with dendrogram construction and analysis of genetic similarity (S). The results showed that the Cr and Pb concentrations in O. niloticus samples ranged from not detected to 0.025 ± 0.001 and not detected to 0.050 ± 0.002 mg/kg, in a fish cultured farm and a stabilization pond, respectively. The Cd concentrations not detected in all fish samples. RAPD was successfully applied to produce 877 total bands used for dendrogram construction and analysis of S. The dendrogram separated O. niloticus into two clusters corresponding to their studied area. The group of O. niloticus sample correlated with Cr and Pb concentrations. The Cr and Pb concentration in O. niloticus was low, it may still affect the genetic differentiation of fish. The genetic differentiation values within the individual fish samples ranged from 0.167 to 0.251 for samples from the fish cultured farm and 0.148–0.207 for samples from the stabilization pond. The genetic differentiation values of each individual fish sample ranged from 0.148 to 0.355. The genetic differentiation may be due to changes in the environment and genetic inheritance from parents of the fish in each sample.

Keywords

Genetic differentiation Chromium Lead Fish Oreochromis niloticus 
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Notes

Acknowledgements

This research was funded by the Research Group on Toxic Substances in Livestock and Aquatic Animals, Faculty of Veterinary Medicine, Khon Kaen University. We also wish to thank the Molecular Systematics Laboratory at the Department of Biology, Faculty of Science, Khon Kaen University for DNA analysis. We are deeply grateful to Professor Dr. Arunrat Chaveerach for her helpful recommendation of our work.

References

  1. 1.
    Ali W, Isner JC, Isayenkov SV, Liu W, Zhao FJ, Maathuis FJ. Heterologous expression of the yeast arsenite efflux system ACR3 improves Arabidopsis thaliana tolerance to arsenic stress. New Phytol. 2012;194:716–23.CrossRefPubMedGoogle Scholar
  2. 2.
    An LH, Zheng BH, Wang LJ, Zhang YQ, Chen H, Zhao XR, Zhang L, Lei K. Biomarker responses and genotoxicity in the mud snail (Bullacta exarata) as indicators of coastal contamination. Mar Pollut Bull. 2012;64:303–9.CrossRefPubMedGoogle Scholar
  3. 3.
    APHA. Standard methods for the examination of water and wastewater. 21st ed. Washington, DC: American Public Health Association; 2005.Google Scholar
  4. 4.
    Bailey RM, Stokes S, Bray H. Inductively coupled plasma mass spectrometry (ICPMS) for dose rate determination: some guidelines for sample preparation and analysis. Oxford: Oxford Luminescence Research Group, School of Geography and the Environment, University of Oxford; 2003.Google Scholar
  5. 5.
    Burrel DC. Atomic spectrometric analysis of heavy metal pollutants in water. Alaska: Institute of Marine Science, University of Alaska Fairbanks; 1977.Google Scholar
  6. 6.
    Cardwell AJ, Hawker DW, Greenway M. Metal accumulation in aquatic macrophytes from Southeast Queensland, Australia. Chemosphere. 2002;48:653–63.CrossRefPubMedGoogle Scholar
  7. 7.
    Devaux A, Fiat L, Gillet C, Bony S. Reproduction impairment following paternal genotoxin exposure in brown trout (Salmo trutta) and Arctic charr (Salvelinus alpinus). Aquat Toxicol. 2011;101:405–11.CrossRefPubMedGoogle Scholar
  8. 8.
    De Wolf H, Blust R, Backeljiau T. The use of RAPD in ecotoxicology: a review. Mutat Res. 2004;566:249–62.CrossRefPubMedGoogle Scholar
  9. 9.
    Environment DG, EU. Working document on sludge 3rd draft. Brussels, 27 Apr 2000. http://ec.europa.eu/environment/waste/sludge/pdf/sludge_en.pdf. Accessed Sept 2014.
  10. 10.
    Gupta M, Sarin NB. Heavy metal induced DNA changes in aquatic macrophytes: random amplified polymorphic DNA analysis and identification of sequence characterized amplified region marker. J Environ Sci. 2009;21:686–90.CrossRefGoogle Scholar
  11. 11.
    Héry M, Gault AG, Rowland HA, Lear G, Polya DA, Lloyd JR. Molecular and cultivation dependent analysis of metal-reducing bacteria implicated in arsenic mobilization in south-east asian aquifers. Appl Geochem. 2008;23:3215–23.CrossRefGoogle Scholar
  12. 12.
    Hosetti BB, Frost S. A review of the sustainable value of effluents and sludges from wastewater stabilization ponds. Ecol Eng. 1995;5:421–31.CrossRefGoogle Scholar
  13. 13.
    Krenkle PA. Heavy metal in the aquatic environment. In: Proceedings of the international conference held in Nashvill, Dec 1975.Google Scholar
  14. 14.
    Lacaze E, Devaux A, Mons R, Bony S, Garric J, Geffard A, Geffard O. DNA damage in caged Gammarus fossarum amphipods: a tool for freshwater genotoxicity assessment. Environ Pollut. 2011;159:1682–91.CrossRefPubMedGoogle Scholar
  15. 15.
    Lewis C, Galloway T. Reproductive consequences of paternal genotoxin exposure in marine invertebrates. Environ Sci Technol. 2009;43:928–33.CrossRefPubMedGoogle Scholar
  16. 16.
    Mara DD. Design manual for sewage lagoons in the tropics. Nairobi: EA Literature Bureau; 1976.Google Scholar
  17. 17.
    Nriagu JO. Production, uses and properties of cadmium. In: Nriagu JO, editor. Cadmium in the environmental. Part I: ecological cycling. New York: Wiley; 1980.Google Scholar
  18. 18.
    Oost R, Beyer J, Vermeulen NPE. Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Pharmacol. 2003;13:57–149.CrossRefPubMedGoogle Scholar
  19. 19.
    Rohlf FJ. NTSYS_pc: Numerical taxonomy and multivariate analysis system, version 2.1, applied biostatistics, New York 1998.Google Scholar
  20. 20.
    Singh A, Sharma RK, Agrawal M, Marshall FM. Risk assessment of heavy metal toxicity through contaminated vegetables from wastewater irrigated area of Varanasi, India. J Trop Ecol. 2010;51:375–87.Google Scholar
  21. 21.
    Thailand Pollution Control Department. The standard levels of heavy metals in tissues of aquatic animals. Bangkok: Notification in Ministry of Public Health No. 98; 1986.Google Scholar
  22. 22.
    Thailand Pollution Control Department. Water quality standards. Bangkok: Notification in Ministry of Public Health No. 98; 2001.Google Scholar
  23. 23.
    Tyler CR, Filby AL, Aerle RV, Lange A, Ball J, Santos EM. Fish toxicogenomics. Adv Exp Biol. 2008;2:75–132.CrossRefGoogle Scholar
  24. 24.
    US Environmental Protection Agency. Sample preparation procedure for spectrochemical determination of total recoverable element, method 200.2. Cincinnati: Environmental Monitoring Systems Lab; 1994.Google Scholar
  25. 25.
    US Environmental Protection Agency. Reviews of the environmental effects of Pollutants: IV. Cadmium: US Environmental Protection Agency, ONRL/EIS-106, EPA–600/1–78–026; 1978.Google Scholar
  26. 26.
    Violante A, Gaudio S, Pigna M, Pucci M, Amalfitano C. Sorption and desorption of arsenic by soil minerals and soils in the presence of nutrients and organics. In: Huang, Q, Huang P, Violante A, editors. Soil mineral microbeorganic interactions. Berlin: Springer; 2008.Google Scholar
  27. 27.
    WHO. Environmental health criteria 165: inorganic lead. Geneva: WHO; 1995.Google Scholar
  28. 28.
    WHO. Environmental health criteria 85: lead environmental aspects. Geneva: WHO; 1989.Google Scholar

Copyright information

© Archana Sharma Foundation of Calcutta 2017

Authors and Affiliations

  • Bundit Tengjaroenkul
    • 1
    • 2
  • Weerayuth Supiwong
    • 1
    • 3
  • Pansa Monkheang
    • 1
    • 4
  • Rattikran Udomphan
    • 1
    • 5
  • Sirilak Boonmee
    • 1
    • 5
  • Sutee Chowrong
    • 1
    • 5
  • Lamyai Neeratanaphan
    • 1
    • 5
  1. 1.Research Group on Toxic Substances in Livestock and Aquatic AnimalsKhon Kaen UniversityKhon KaenThailand
  2. 2.Department of Veterinary Medicine, Faculty of Veterinary MedicineKhon Kaen UniversityKhon KaenThailand
  3. 3.Faculty of Applied Science and EngineeringKhon Kaen UniversityNong KhaiThailand
  4. 4.Department of Biology, Faculty of ScienceKhon Kaen UniversityKhon KaenThailand
  5. 5.Department of Environmental Science, Faculty of ScienceKhon Kaen UniversityKhon KaenThailand

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