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The Nucleus

, Volume 58, Issue 3, pp 225–230 | Cite as

Further observations on chromosome diversity analysis in wild species of Vigna from India

  • Anju Shamurailatpam
  • Daniel Regie Wahlang
  • Latha Madhavan
  • Shrirang Ramachandra Yadav
  • Kangila Venkataraman Bhat
  • Satyawada Rama RaoEmail author
Original Article

Abstract

Ten wild species of Vigna from the Indian gene centre have been karyo-morphologically analysed. All the studied taxa had somatic chromosome number of 2n = 22 except V. glabrescens which had 2n = 44. The chromosomes were found to be either metacentric, submetacentric and subtelocentric in type with their respective chromosome complements, and the complete absence of telocentric chromosomes in all of the taxa studied. The karyotypes of the species were mostly symmetrical. The significance of karyotypic variation among wild species in comparison with the cultivated ones of the genus Vigna has been discussed in detail. Such information has value in understanding cytogenetical relationship amongst these wild species reported to comprise of genes for resistance to insect, pests and diseases.

Keywords

VignWild species Karyotype Asymmetry index NOR-chromosomes 

Abbreviations

NOR

Nuclear Organizing Region

AI

Asymmetry index

SC

The shortest chromosome length

LC

The longest chromosome length

CL

Mean length of chromosome

CI

Mean centromeric index

SD

Standard Deviation

CVCL

Component expressing the relative variation in chromosome length

CVCI

Component expressing the relative variation in centromeric index

References

  1. 1.
    Arora RK. Diversity and collection of wild Vigna species in India. FAO/IBPGR Plant Genet Res Newslet. 1985;63:26–33.Google Scholar
  2. 2.
    Battaglia E. Chromosome morphology and terminology. Caryologia. 1955;6:179–87.CrossRefGoogle Scholar
  3. 3.
    Bisht IS, Bhat KV, Lakhanpaul S, Latha M, Jayan PK, Biswas BK, Singh AK. Diversity and genetic resources of wild Vigna species in India. Genet Resour Crop Evol. 2005;52:53–68.CrossRefGoogle Scholar
  4. 4.
    Chandel KPS, Joshi BS, Pant KC. Genetic resources of Vigna species in India, their distribution, diversity and utilization in crop improvement. Ann Agric Res. 1982;3:19–34.Google Scholar
  5. 5.
    Goel S, Raina SN, Ogihara Y. Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of nuclear ribosomal DNA in the Phaseolus-Vigna complex. Mol Phylogenet Evol. 2002;22:1–19.CrossRefPubMedGoogle Scholar
  6. 6.
    Hynneiwta M, Malik SK, Rao SR. Karyological studies in ten species of Citrus (linnaues, 1753) (rutaceae) of North East India. Comp Cytogen. 2011;5:277–87.CrossRefGoogle Scholar
  7. 7.
    Kumar A, Rao SR. Cytological investigations in some important tree species of Rajasthan I. Karyomorphological studies in some species of Anogeissus (DC.) Guill., Perr. & A. Rich. Silvae Genet. 2002;51:100–4.Google Scholar
  8. 8.
    Ladeinde TAO, Raharajeswari S, Oguike J, Cole T. The karyotype of meiotic chromosomes of cowpea (Vigna unguiculata (L. Walp) Nigerian. J Basic Appl Sci. 1980;14:1–2.Google Scholar
  9. 9.
    Lewis GP, Schrine B, Mackinder B, Lock JM. Legumes of the world. Kew: Royal Botanic Gardens; 2005.Google Scholar
  10. 10.
    Matsubayashi O. Phylogenetic relationships in the potato and its related species. In: Tsuchiya T, Gupta PK. (eds.): Chromosome engineering in plants: genetics, breeding, evolution, Elsevier, Amsterdam, Netherlands. part B;1991. p. 93118.Google Scholar
  11. 11.
    Paszko B A critical review and a new proposal of karyotype asymmetry indices. Plant Syst and Evol. 2006;258:39–48.CrossRefGoogle Scholar
  12. 12.
    Raina SN, Mukai Y. Detection of a variable number of 18S-5.8S-26S and 5S ribosomal DNA loci by fluorescent in situ hybridization in diploid and tetraploid Arachis species. Genome. 1999;42:52–9.CrossRefGoogle Scholar
  13. 13.
    Rao SR, Chandel KPS. Karyomorphological studies in the cultivated and wild vigna species in Indian gene Centre. Cytologia. 1991;56:47–57.CrossRefGoogle Scholar
  14. 14.
    Rao SR, Raina SN. Studies on male meiosis in cultivated and wild Vigna species. In: Srivastava PS, Narula A, Srivastava S, editors. Plant biotechnology and molecular markers. Netherlands: Springer; 2004. p. 331–45.Google Scholar
  15. 15.
    Rawat D, Sharma SK, Mahmoudi A, Rao SR. Cytogenetic rationale for probable amphidiploid origin of Dipcadi erythraeum Webb. & berth. - a rare and endemic plant of Indian Thar desert. Caryologia. 2011;64:75–83.CrossRefGoogle Scholar
  16. 16.
    Shamurailatpam A. Molecular Cytogenetic Studies on Inter- and Intraspecific Variation in Vigna Species from North-East India. PhD. Thesis, North-Eastern Hill University, Shillong; 2014.Google Scholar
  17. 17.
    Shamurailatpam A, Madhavan L, Yadav SR, Bhat KV, Rao SR. Chromosome diversity analysis in various species of Vigna savi from India. Nucleus. 2012;55:107–14.CrossRefGoogle Scholar
  18. 18.
    Shamurailatpam A, Madhavan L, Yadav SR, Bhat KV, Rao SR. Heterochromatin characterization through differential fluorophore binding pattern in some species of Vigna savi. Protoplasma. 2015;252:629–35.CrossRefPubMedGoogle Scholar
  19. 19.
    Shamurailatpam A, Madhavan L, Yadav SR, Bhat KV, Rao SR. Heterochromatin distribution and comparative karyo-morphological studies in Vigna umbellata Thunberg, 1969 and V. aconitifolia jacquin, 1969 (fabaceae) accessions. Comp Cytogen. 2015;9:119–32.CrossRefGoogle Scholar
  20. 20.
    Sharma SK, Rajkumari K, Kumaria S, Tandon P, Rao SR. Karyomorphological characterization of natural genetic variation in some threatened Cymbidium species of northeast India. Caryologia. 2010;63:99–105.CrossRefGoogle Scholar
  21. 21.
    Singh A, Roy RP. Karyological studies in Trigonella, Indigofera and Phaseolus. The Nucleus. 1970;13:41–54.Google Scholar
  22. 22.
    Singh RJ. The handling of plant chromosomes: Plant Cytogenetics.CRC Press; 1993.Google Scholar
  23. 23.
    Stace CA. Cytology and cytogenetics as a fundamental taxonomic resource for the 20th and 21st centuries. Taxon. 2000;49:451–77.CrossRefGoogle Scholar
  24. 24.
    Stebbins GL. Chromosomal evolution in higher plants. London: Edward Arnold Ltd.; 1971.Google Scholar

Copyright information

© Archana Sharma Foundation of Calcutta 2016

Authors and Affiliations

  • Anju Shamurailatpam
    • 1
  • Daniel Regie Wahlang
    • 1
  • Latha Madhavan
    • 2
  • Shrirang Ramachandra Yadav
    • 3
  • Kangila Venkataraman Bhat
    • 4
  • Satyawada Rama Rao
    • 1
    Email author
  1. 1.Department of Biotechnology and BioinformaticsNorth Eastern Hill UniversityShillongIndia
  2. 2.National Bureau of Plant Genetic Resources, Regional StationVellanikkaraThrissurIndia
  3. 3.Department of BotanyShivaji UniversityKolhapurIndia
  4. 4.National Research Centre on DNA FingerprintingNational Bureau of Plant Genetic ResourcesNew Delhi,India

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