Biodegradable and Porous Poly(lactic-co-glycolic acid) Microbeads for In vitro Evaluation of Negatively Charged Fluorescent Bacteria
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In this study, porous microbeads synthesized by water-oil-water [water/ oil/water (W1/O/W2)] double-emulsion solvent evaporation method exhibited immense potential as biocarriers for the attachment of bacteria. Poly(lactic-co-glycolic acid) (PLGA) was selected as a biodegradable, biocompatible material for fabricating porous microbeads. Ammonium bicarbonate and polyvinyl alcohol concentrations played a significant role in the modification of the microbead size. The microbead surface structure was modified by partial hydrolysis using sodium hydroxide to generate open pores on the surface and a more hydrophilic surface than PLGA itself. To examine the inner morphology of the microbeads, confocal laser scanning microscopy was employed in combination with rhodamine B during the fabrication of porous microbeads. Finally, the attachment of fluorescent bacteria to these porous microbeads was analyzed in terms of affinity between bacteria and porous PLGA microbeads. The affinity of microbeads to negatively charged bacteria was observed as a function of the charges of the PLGA microbead surfaces, indicating that surface-modified PLGA porous microbeads decorated with positively charged chitosan exhibit an enhanced affinity to negatively charged bacteria.
KeywordsPLGA porous microbeads surface modification bacterial attachment
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- (20).T. S. Lee, R. A. Krupa, F. Zhang, M. Hajimorad, W. J. Holtz, N. Prasad, S. K. Lee, and J. D. Keasling, J. Biol. Eng., 5, 12 (2011).Google Scholar
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