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Macromolecular Research

, Volume 26, Issue 12, pp 1115–1122 | Cite as

Cellular Delivery of siRNA Using Poly(2-dimethylaminoethyl methacrylate)- Functionalized Graphene Oxide Nano-Wrap

  • Jung Eun Lee
  • Kyuri Lee
  • Jeong A. Nam
  • Aesun Kim
  • So Young Lee
  • Min Sang Lee
  • Nak Won Kim
  • Yue Yin
  • Ji Won Park
  • Sung Young ParkEmail author
  • Ji Hoon JeongEmail author
Article
  • 93 Downloads

Abstract

Despite its high therapeutic potential, the use of siRNA has still been limited mostly due to the lack of a proper delivery system. Small number of charges and inflexible structure of siRNA are the major factors that affect cellular delivery efficiency of conventional lipid and polymeric carriers. In this study, we synthesized a 2-dimensional (2D) carrier for siRNA using a facile method to functionalize and stabilize the surface of graphene oxide (GO) with catechol-derivatized poly(2-dimethylaminoethyl methacrylate) (C-p(DMAEMA)). The resulting GO/C-p(DMAEMA) can interact with siRNA on the flexible 2D surface of the GO, wrapping up siRNA molecules by folding to form a dim sum-like nano structure. GO/C-p(DMAEMA) demonstrated significantly improved cellular uptake and enhanced gene silencing efficiency in the presence of serum protein, compared to unmodified GO and bPEI 25 kDa.

Keywords

non-viral gene delivery siRNA graphene oxide nano-wrap dim sum-like structure 

Supplementary material

13233_2019_7017_MOESM1_ESM.pdf (310 kb)
Supporting Information

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Copyright information

© The Polymer Society of Korea and Springer Nature B.V. 2018

Authors and Affiliations

  • Jung Eun Lee
    • 1
  • Kyuri Lee
    • 1
  • Jeong A. Nam
    • 2
  • Aesun Kim
    • 1
  • So Young Lee
    • 2
  • Min Sang Lee
    • 1
  • Nak Won Kim
    • 1
  • Yue Yin
    • 1
  • Ji Won Park
    • 1
  • Sung Young Park
    • 2
    Email author
  • Ji Hoon Jeong
    • 1
    Email author
  1. 1.School of PharmacySungkyunkwan UniversitySuwonKorea
  2. 2.Department of Chemical and Biological EngineeringKorea National University of TransportationChungjuKorea

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