Photochemical Studies and Photoinduced Antibacterial Properties of Silver Nanoparticle-Encapsulated Biomacromolecule Bovine Serum Albumin Functionalised with Photoresponsive Chromophoric System 2-[(E)-(3-Hydroxynaphthalen-2-yl) diazenyl] Benzoic Acid
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This study establishes the synthesis of silver nanoparticles (AgNPs), synthesis of a photoresponsive system 2-[(E)-(3-hydroxynaphthalen-2-yl) diazenyl] benzoic acid, and encapsulation of AgNPs into the biomacromolecular system, bovine serum albumin (BSA) functionally modified with the photoactive system by means of DCC coupling. The optical properties, structural properties, morphology, and size distribution were confirmed by various characterisation techniques such as ultraviolet (UV)/visible, Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), photoluminescence (PL) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction analysis (XRD). The photo responsive behaviour of the functionally modified BSA and nanoparticle dispersed system were investigated. The antibacterial effects of AgNPs and photo induced antimicrobial properties of the functionally modified BSA-AgNP conjugates were evaluated against some selected bacterial strains such as Corynebacterium diphtheriae (gram +ve), Bacillus cereus (gram +ve), Raoultella ornithinolytica (gram -ve), and Salmonella typhimurium (gram -ve) using disc diffusion. We found that silver nanoparticles encapsulating functionally modified BSA seem to be an effective photoactive antimicrobial agent against the multidrug resistant strains of bacteria with better photo responsive properties and with wide applications in antimicrobial photodynamic therapy (APDT).
Keywordssilver nanoparticle 2-[(E)-(3-hydroxynaphthalen-2-yl) diazenyl] benzoic acid bovine serum albumin DCC coupling encapsulation
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- (12).I. M. Tynga, A. Hussein, M. A. Harith, and H. Abrahamse, Int. J. Nanomedicine, 9, 3771 (2014).Google Scholar
- (20).Clinical and Laboratory Standards Institute (CLSI), Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard, 3rd ed., M31-A3, Vol. 28:8 (2008).Google Scholar
- (21).T. Pradeep, NANO the Essentials, Tata McGraw-Hill Education, New Delhi, 2007.Google Scholar
- (22).B. D. Cullity, Elements of X-ray Diffraction, 2nd ed., Edison-Wesley Publishing Company Inc, USA, 1978.Google Scholar