Macromolecular Research

, 19:911 | Cite as

Control of adhesion, focal adhesion assembly, and differentiation of myoblasts by enzymatically crosslinked cell-interactive hydrogels

  • Indong Jun
  • Kyung Min Park
  • Dong Yun Lee
  • Ki Dong Park
  • Heungsoo Shin
Articles
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Abstract

The development of an artificial matrix is critical as both a substrate to control the cell behavior and as a tool for examining the roles of cellular microenvironment in biology. This study developed cell-interactive hydrogels containing a Arg-Gly-Asp (RGD) peptide, cross-linked via bio-inspired enzymatic processes using H2O2 and horseradish peroxidase (HRP) as the initiators and examined how they controlled myoblast functions. The cell-interactive hydrogels modulated the adhesion and proliferation of myoblast, depending on the peptide density. Furthermore, the expression of focal adhesion proteins and myogenic differentiation were up-regulated significantly in myoblasts cultured on peptide-incorporated hydrogels. Therefore, the novel hydrogel system can be used to regulate the cell function for many tissue engineering applications.

Keywords

in situ gelation enzyme polymerization RGD peptide myoblast adhesion myogenic differentiation 
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Copyright information

© The Polymer Society of Korea and Springer Netherlands 2011

Authors and Affiliations

  • Indong Jun
    • 1
  • Kyung Min Park
    • 2
  • Dong Yun Lee
    • 1
  • Ki Dong Park
    • 2
  • Heungsoo Shin
    • 1
    • 3
    • 4
  1. 1.Department of BioengineeringHanyang UniversitySeoulKorea
  2. 2.Department of Molecular Science and TechnologyAjou UniversityGyeonggiKorea
  3. 3.Institute for Bioengineering and Biopharmaceutical ResearchHanyang UniversitySeoulKorea
  4. 4.Institute of Aging SocietyHanyang UniversitySeoulKorea

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