Macromolecular Research

, Volume 18, Issue 7, pp 630–635 | Cite as

Insulin-incorporated chitosan nanoparticles based on polyelectrolyte complex formation

  • Joung-Pyo Nam
  • Changyong Choi
  • Mi-Kyeong Jang
  • Young-Il JeongEmail author
  • Jae-Woon NahEmail author
  • Sung-Hyun Kim
  • Yoonkyung Park


Insulin-incorporated nanoparticles were prepared by polyelectrolyte complex formation using low-molecular weight water soluble chitosan (LMWSC). Insulin-incorporated nanoparticles showed spherical shapes with a particle size of approximately 200 nm. The average particle size, drug content and loading efficiency increased with increasing insulin feed. The zeta potential decreased with increasing insulin feed, indicating that the electrostatic interaction increased with increasing insulin feed. At 1H nuclear magnetic resonance (NMR) spectra, insulin and LMWSC had intrinsic peaks while the peaks of the nanoparticles were similar to LMWSC itself at D2O. However, the specific peaks for insulin appeared as a result of the breakdown of nanoparticles using DCl. When polyelectrolyte complex had dissociated, fluorescein isothiocyanate (FITC)-labeled insulin in the nanoparticles showed similar UV absorption peaks to FITC-labeled insulin only. At the release study, insulin was released continuously from the nanoparticles over 120 h at all formulations. Higher insulin contents induced a decrease in the rate of insulin released from nanoparticles. Consequently, LMWSC and insulin successively formed polyelectrolyte complexes as a nanocarriers and LMWSC can be considered a good candidate for insulin delivery.


chitosan insulin nanoparticle polyelectrolyte complex controlled release 


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  1. (1).
    S. Hirano, Polym. Int., 48, 734 (1999).CrossRefGoogle Scholar
  2. (2).
    J. M. Dang and K. W. Leong, Adv. Drug Del. Rev., 58, 499 (2006).CrossRefGoogle Scholar
  3. (3).
    M. Thanou, J. C. Verhoef, and H. E. Junginger, Adv. Drug Del. Rev., 52, 126 (2001).CrossRefGoogle Scholar
  4. (4).
    T. W. Chung, J. Yang, T. Akaike, K. Y. Cho, J. W. Nah, S. I. Kim, and C. S. Cho, Biomaterials, 23, 2834 (2002).Google Scholar
  5. (5).
    S. Y. Chae, M. K. Jang, and J. W. Nah, J. Control. Release, 102, 394 (2005).CrossRefGoogle Scholar
  6. (6).
    S. Y. Chae, S. Son, M. Lee, M. K. Jang, and J. W. Nah, J. Control. Release, 109, 344 (2005).CrossRefGoogle Scholar
  7. (7).
    D. G. Kim, C. Y. Choi, Y. I. Jeong, M. K. Jang, J. W. Nah, S. K. Kang, and M. S. Bang, Macromol. Res., 14, 72 (2006).Google Scholar
  8. (8).
    P. Calvo, C. Remuñan-López, J. L. Vila-Jato, and M. J. Alonso, Pharm. Res., 14, 1436 (1997).CrossRefGoogle Scholar
  9. (9).
    R. Fernández-Urrusuno, P. Calvo, C. Remuñán-López, J. L. Vila-Jato, and M. J. Alonso, Pharm. Res., 16, 1581 (1999).CrossRefGoogle Scholar
  10. (10).
    B. Sarmento, A. Ribeiro, F. Veiga, P. Sampaio, R. Neufeld, and D. Ferreira, Pharm. Res., 24, 2206 (2007).CrossRefGoogle Scholar
  11. (11).
    J. W. Bae, D. H. Go, K. D. Park, and S. J. Lee, Macromol. Res., 14, 461 (2006).Google Scholar
  12. (12).
    Y. I. Jeong, D. G. Kim, M. K. Jang, and J. W. Nah, Carbohydr. Res., 343, 289 (2008).CrossRefGoogle Scholar
  13. (13).
    D. G. Kim, Y. I. Jeong, and J. W. Nah, J. Appl. Polym. Sci., 105, 3254 (2007).Google Scholar
  14. (14).
    M. Lee, J. W. Nah, Y. Kwon, J. J. Koh, K. S. Ko, and S. W. Kim, Pharm. Res., 18, 431 (2001).Google Scholar

Copyright information

© The Polymer Society of Korea and Springer Netherlands 2010

Authors and Affiliations

  • Joung-Pyo Nam
    • 1
  • Changyong Choi
    • 1
  • Mi-Kyeong Jang
    • 1
  • Young-Il Jeong
    • 1
    Email author
  • Jae-Woon Nah
    • 1
    Email author
  • Sung-Hyun Kim
    • 2
  • Yoonkyung Park
    • 3
  1. 1.Department of Polymer Science and EngineeringSunchon National UniversityJeonnamKorea
  2. 2.Regional Office of Kwangju-Chonnam Small and Medium Business AdministrationGwangjuKorea
  3. 3.Department of Biotechnology College of Natural ScienceChosun UniversityGwang-juKorea

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