Fungal Diversity

, Volume 58, Issue 1, pp 215–226 | Cite as

Borofutus, a new genus of Boletaceae from tropical Asia: phylogeny, morphology and taxonomy

  • Md. Iqbal Hosen
  • Bang Feng
  • Gang Wu
  • Xue Tai Zhu
  • Yan Chun Li
  • Zhu L. Yang


A new monotypic genus in the Boletaceae, Borofutus, typified by B. dhakanus, is described using morphological and molecular evidence. This is a putatively ectomycorrhizal fungus associated with Shorea robusta. Borofutus is characterized by the combination of the following characters: basidiomata small to medium-sized; pileus grayish brown to cocoa brown; hymenophore subdecurrent, cream then golden brown, with broad, nearly hexagonal pores; basidiospores purple to purplish red in H2O, ornamented with irregular to regular shallow pits; cystidia lageniform, thick-walled. Borofutus is sister to Spongiforma in molecular phylogenetic analyses using DNA nucleotide sequences of single or multiple loci. A description, line drawings, phylogenetic placement and comparison with allied taxa are presented herein.


Boletes Dipterocarpaceae Multiple gene analysis New fungal taxon 



The first author is grateful to Md. Abbas Ali and Md. Abdus Samad, Thakurgaon, Bangladesh for the field investigation and collection of the specimens. Special thanks to Prof. Dr. Dennis E. Desjardin, Department of Biology, San Francisco State University, California, USA and Prof. Dr. Donald H. Pfister, Farlow Herbarium of Harvard University, USA for providing the type materials of Spongiforma thailandica and Austroboletus tristis, respectively. The authors are thanked to Jacqueline Van De Veire, Chengdu Institute of Biology, Chengdu, Sichuan, China for polishing the manuscript. Acknowledgements extend to Dr. Su-See Lee, Forest Research Institute Malaysia, Selangor, Malaysia for providing literature. The authors thank to Dr. Maria Alice Neves, Universidade Federal de Santa Catarina, Florianópolis, Brazil for providing her newly generated sequences of Phylloporus pumilus and Dr. N. K. Zeng, Department of Pharmacy, Hainan Medical University, China for providing SEM images of Austroboletus tristis. The authors are very grateful to reviewers Dr. Roy E. Halling, Institute of Systematic Botany, the New York Botanical Garden, USA and Dr. Else C. Vellinga, Department of Plant and Microbial Biology, University of California, USA for their constructive comments and suggestions. This study was supported by the Funds for International Cooperation and Exchange of the National Natural Science Foundation of China (No. 31210103919), the National Basic Research Program of China (No. 2009CB522300), the Hundred Talents Program of the Chinese Academy of Sciences, and the Ministry of Science and Technology of China (2008FY110300).


  1. Besl H, Bresinsky A (1997) Chemosystematics of Suillaceae and Gomphidiaceae (suborder Suillineae). Pl Syst Evol 206:223–242CrossRefGoogle Scholar
  2. Binder M, Besl H (2000) 28S rDNA sequence data and chemotaxonomical analyses on the generic concept of Leccinum (Boletales). A.M.B., Italy. Centro Studi Micologici. Micologia 2000:71–82Google Scholar
  3. Binder M, Bresinsky A (2002a) Derivation of a polymorphic lineage of Gasteromycetes from boletoid ancestors. Mycologia 94:85–98PubMedCrossRefGoogle Scholar
  4. Binder M, Bresinsky A (2002b) Retiboletus, a new genus for a species-complex in the Boletaceae producing retipolides. Feddes Repertorium 113:30–40CrossRefGoogle Scholar
  5. Binder M, Fischer M (1997) Molekularbiologische Charakterisierung der Gattungen Boletellus und Xerocomus: Xerocomus pruinatus (Fr. & Hök) Quél. und verwandte Arten. Boll Gruppo Micol G Bresadola 40:79–90Google Scholar
  6. Binder M, Hibbett DS (2007) Molecular systematics and biological diversification of Boletales. Mycologia 98:971–981. doi: 10.3852/mycologia.98.6.971 CrossRefGoogle Scholar
  7. Binder M, Larsson KH, Matheny PB, Hibbett DS (2010) Amylocorticiales ord. nov. and Jaapiales ord. nov.: early diverging clades of Agaricomycetidae dominated by corticioid forms. Mycologia 102:865–880. doi: 10.3852/09-288 PubMedCrossRefGoogle Scholar
  8. Bresinsky A, Jarosch M, Fischer M, Schonberger I, Wittmann-Bresinsky B (1999) Phylogenetic relationships within Paxillus s. l. (Basidiomycetes, Boletales): separation of a Southern Hemisphere genus. Plant Biol 1:27–333CrossRefGoogle Scholar
  9. Corner EJH (1972) Boletus in Malaysia. Singapore Government Printer, Singapore, pp 1–263Google Scholar
  10. Dentinger BTM, Ammirati JF, Both EE, Desjardin DE, Halling RE, Henkel TW, Moreau PA, Nagasawa E, Soytong K, Taylor AF, Watling R, Moncalvo JM, McLaughlin DJ (2010) Molecular phylogenetics of porcini mushrooms (Boletus section Boletus). Mol Phylogenet Evol 57:1276–1292PubMedCrossRefGoogle Scholar
  11. Desjardin DE, Wilson AW, Binder M (2008) Durianella, a gasteroid genus of boletes from Malaysia. Mycologia 100:956–961. doi: 10.3852/08-062 PubMedCrossRefGoogle Scholar
  12. Desjardin DE, Binder M, Roekring S, Flegel T (2009) Spongiforma, a new genus of gasteroid boletes from Thailand. Fungal Divers 37:1–8Google Scholar
  13. Desjardin DE, Peay KG, Bruns TD (2011) Spongiforma squarepantsii, a new species of gasteroid bolete from Borneo. Mycologia 103:1119–1123. doi: 10.3852/10-433 PubMedCrossRefGoogle Scholar
  14. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  15. Drehmel D, James T, Vilgalys R (2008) Molecular phylogeny and biodiversity of the boletes. Fungi 1:17–23Google Scholar
  16. Feng B, Xu J, Wu G, Zeng NK, Hosen MI, Li YC, Tolgor B, Kost GW, Yang ZL (2012) DNA sequence analyses reveal abundant diversity, endemism and evidence for Asian origin of the porcini mushrooms. PLoS One 7:e37567. doi: 10.1371/journal.pone.0037567 PubMedCrossRefGoogle Scholar
  17. Fulgenzi TD, Halling RE, Henkel TW (2010) Fistulinella cinereoalba sp. nov. and new distribution records for Austroboletus from Guyana. Mycologia 102:224–232PubMedCrossRefGoogle Scholar
  18. Grubisha LC, Trappe JM, Molina R, Spatafora JW (2002) Biology of the ectomycorrhizal genus Rhizopogon. VI. Re-examination of infrageneric relationships inferred from phylogenetic analyses of ITS sequences. Mycologia 94:607–619PubMedCrossRefGoogle Scholar
  19. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  20. Halling RE, Fechner NA (2011) Heimioporus in Australia. Aust Mycologist 29:47–51Google Scholar
  21. Halling RE, Mueller GM (2003) Leccinum (Boletaceae) in Costa Rica. Mycologia 95:488–499PubMedCrossRefGoogle Scholar
  22. Halling RE, Osmundson TW, Neves MA (2006) Austroboletus mutabilis sp. nov. from northern Queensland. Muelleria 24:31–36Google Scholar
  23. Halling RE, Baroni TJ, Binder M (2007) A new genus of Boletaceae from eastern North America. Mycologia 99:310–316PubMedCrossRefGoogle Scholar
  24. Heinemann P, Rammeloo J (1987a) Novitates generis Phyllopori (Boletineae). Bulletin du Jardin Botanique National de Belgique 57:272–274CrossRefGoogle Scholar
  25. Heinemann P, Rammeloo J (1987b) Phylloporus (Boletineae). Flore Illustreé des Champignons d’ Afrique Centrale 13:277–309Google Scholar
  26. Horak E (2004) Heimioporus E. Horak gen. nov.—replacing Heimiella Boedijn (1951, syn. post., Boletales, Basidiomycota). Sydowia 56:237–240Google Scholar
  27. Horak E (2011) Revision of Malaysian species of Boletales s.l. (Basidiomycota) described by E.J.H. Corner (1972, 1974). Malay For Rec 51:1–283Google Scholar
  28. Katoh K, Kuma K, Toh H, Miyata T (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Res 33:511–518PubMedCrossRefGoogle Scholar
  29. Kornerup A, Wanscher JH (1981) Taschenlexikon der Farben, 3rd edn. Muster-Schmidt, Verlag, pp 1–242Google Scholar
  30. Lebel T, Orihara T, Maekawa N (2012) The sequestrate genus Rossbeevera T. Lebel & Orihara gen. nov. (Boletaceae) from Australasia and Japan: new species and new combinations. Fungal Divers 52:49–71. doi: 10.1007/s13225-011-0109-x CrossRefGoogle Scholar
  31. Leonardi M, Paolocci F, Rubini A, Simonini G, Pacioni G (2005) Assessment of inter- and intra-specific variability in the main species of Boletus edulis complex by ITS analysis. FEMS Microbiol Lett 243:411–416PubMedCrossRefGoogle Scholar
  32. Li YC, Feng B, Yang ZL (2011) Zangia, a new genus of Boletaceae supported by molecular and morphological evidence. Fungal Divers 49:125–143. doi: 10.1007/s13225-011-0096-y CrossRefGoogle Scholar
  33. Matheny PB (2005) Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe, Agaricales). Mol Phylogenet Evol 35:1–20PubMedCrossRefGoogle Scholar
  34. Matheny PB, Curtis JM, Hofstetter V, Aime MC, Moncalvo JM, Ge ZW, Yang ZL, Slot JC, Ammirati JF, Baroni TJ, Bougher NL, Hughes KW, Lodge DJ, Kerrigan RW, Seidl MT, Aanen DK, DeNitis D, Daniele GM, Desjardin DE, Kropp BR, Norvell LL, Parker A, Vellinga EC, Vilgalys R, Hibbett DS (2006) Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98:982–995PubMedCrossRefGoogle Scholar
  35. Matheny PB, Wang Z, Binder M, Curtis JM, Lim YW, Nilsson RH, Hughes KW, Hofstetter V, Ammirati JF, Schoch CL, Langer E, Langer G, McLaughlin DJ, Wilson AW, Frøslev T, Ge ZW, Kerrigan RW, Slot JC, Yang ZL, Baroni TJ, Fischer M, Hosaka K, Matsuura K, Seidl MT, Vauras J, Hibbett DS (2007) Contributions of rpb2 and tef1 to the phylogeny of mushrooms and allies (Basidiomycota, Fungi). Mol Phylogenet Evol 43:430–451PubMedCrossRefGoogle Scholar
  36. Neves MA, Binder M, Halling RE, Soytong K (2012) The phylogeny of selected Phylloporus species, inferred from nrLSU and ITS sequences, and descriptions of new species from the Old World. Fungal Divers 55:109–123. doi: 10.1007/s13225-012-0154-0 CrossRefGoogle Scholar
  37. Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala UniversityGoogle Scholar
  38. Ortiz-Santana B, Lodge DJ, Baroni TJ, Both EE (2007) Boletes from Belize and the Dominican Republic. Fungal Divers 27:247–416Google Scholar
  39. Peintner U, Ladurner H, Simonini G (2003) Xerocomus cisalpinus sp. nov., and the delimitation of species in the X. chrysenteron complex based on morphology and rDNA-LSU sequences. Mycol Res 107:659–679PubMedCrossRefGoogle Scholar
  40. Porter TM, Skillman JE, Moncalvo JM (2008) Fruiting body and soil rDNA sampling detects complementary assemblage of Agaricomycotina (Basidiomycota, Fungi) in a hemlock-dominated forest plot in southern Ontario. Mol Ecol 17:3037–3050PubMedCrossRefGoogle Scholar
  41. Rehner SA, Buckley EP (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97:84–98PubMedCrossRefGoogle Scholar
  42. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574PubMedCrossRefGoogle Scholar
  43. Singer R (1945) The Boletineae of Florida with notes on extralimital species I. The Strobilomycetaceae. Farlowia 2:97–141Google Scholar
  44. Singer R (1986) The Agaricales in modern taxonomy, 4th edn. Koeltz Scientific Books, Königstein, pp 1–981Google Scholar
  45. Smith SA, Dunn CW (2008) Phyutility: a phyloinformatics tool for trees, alignments and molecular data. Bioinformatics 24:715–716PubMedCrossRefGoogle Scholar
  46. Smith AH, Thiers HD (1971) The Boletes of Michigan. University of Michigan Press, Ann Arbor, pp 1–419Google Scholar
  47. Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690PubMedCrossRefGoogle Scholar
  48. Swofford DL (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sinauer Associates, SunderlandGoogle Scholar
  49. Takahashi H, Degawa Y (2011) Two new species of Agaricales and a new Japanese record for Boletellus betula from Japan. Mycoscience 52:312–318. doi: 10.1007/s10267-011-0109-4 CrossRefGoogle Scholar
  50. Thiers HD (1975) California mushrooms. A field guide to the boletes. Hafner Press, New York, p 261Google Scholar
  51. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246PubMedGoogle Scholar
  52. Watling R (1970) Boletaceae: Gomphidiaceae: Paxillaceae. In: Henderson DM, Orton PD, Watling R (eds) British fungus flora. Agarics and Boleti I. Royal Botanical Garden, Edinburgh, p 124Google Scholar
  53. Watling R, Hollands R (1990) Boletes from Sarawak. Notes Royal Bot Gard Edinb 46:405–422Google Scholar
  54. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols: a guide to methods and applications. Academic, San Diego, pp 315–322Google Scholar
  55. Wolfe CB Jr (1979) Austroboletus and Tylopilus subg. Porphyrellus with emphasis on North American taxa. Biblioth Mycol 69:1–148Google Scholar
  56. Wolfe CB Jr, Bougher NL (1993) Systematics, mycogeography, and evolutionary history of Tylopilus subg. Roseoscabra in Australia elucidated by comparison with Asian and American species. Austral Syst Bot 6:187–213CrossRefGoogle Scholar
  57. Wolfe CB Jr, Petersen RH (1978) Taxonomy and nomenclature of the supraspecific taxa of Porphyrellus. Mycotaxon 7:152–162Google Scholar
  58. Yang ZL (2011) Molecular techniques revolutionize knowledge of basidiomycete evolution. Fungal Divers 50:47–58. doi: 10.1007/s13225-011-0121-1 CrossRefGoogle Scholar
  59. Zeng NK, Cai Q, Yang ZL (2012) Corneroboletus, a new genus to accommodate the southeastern Asian Boletus indecorus. Mycologia. doi: 10.3852/11-326

Copyright information

© Mushroom Research Foundation 2012

Authors and Affiliations

  • Md. Iqbal Hosen
    • 1
    • 2
  • Bang Feng
    • 1
  • Gang Wu
    • 1
    • 2
  • Xue Tai Zhu
    • 1
    • 2
  • Yan Chun Li
    • 1
  • Zhu L. Yang
    • 1
  1. 1.Key Laboratory of Biodiversity and Biogeography, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

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