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Fungal Diversity

, Volume 54, Issue 1, pp 69–77 | Cite as

Diversity of foliar endophytes in wind-fallen Picea abies trees

  • Ondřej Koukol
  • Miroslav Kolařík
  • Zuzana Kolářová
  • Petr Baldrian
Article

Abstract

The unmanaged Norway spruce montane forests in the Bohemian Forest National Park (Czech Republic) suffered from repeated large-scale bark beetle outbreaks in last decade. In this study, the diversity of culturable foliar endophyte microfungi in needles originating from eleven recently wind-fallen trees in this area was surveyed. Our aims were to describe their diversity and to determine the relative host and organ specificities of isolated endophyte species to estimate the species pool and abundance of foliar endophytes before the forest degradation. Microfungi were isolated from surface-sterilized needles, and the outgrowing strains were identified based on morphological and molecular characteristics (analyses of ITS1, ITS2 and partial 28S rDNA). Fungal communities in the needles were diverse, with ascomycetes (mostly anamorphs of Helotiales) dominating basidiomycetes. The most frequent species (Phacidiopycnis sp., Cistella acuum, Sirococcus sp. and two species of Chalara) did not correspond with those recorded in previous studies of Picea spp. For example, the widely distributed Lophodermium piceae was rarely recorded in this study. This pattern may be caused by different methods of sterilization and cultivation or by physiological characteristics of the needles, or it may reflect the species distribution in the studied area. Members of the Helotiales, along with sequences from GenBank, showed substantial overlap in host affinities, most prominently between Pinus and Picea, and also among species from distant plant lineages.

Keywords

Morphospecies ITS rDNA Culturable microfungi Phacidiopycnis Host affinity 

Notes

Acknowledgements

This study was supported by the Grant Agency of the Czech Republic (project Nr. 526/08/0751) and by the Ministry of Education, Youth and Sports of the Czech Republic (project No. MSM0021620828). PB was supported by the Institutional Research Concept of the Institute of Microbiology of the ASCR, v.v.i. (AV0Z50200510).

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Copyright information

© Kevin D. Hyde 2011

Authors and Affiliations

  • Ondřej Koukol
    • 1
  • Miroslav Kolařík
    • 1
    • 2
  • Zuzana Kolářová
    • 1
    • 3
  • Petr Baldrian
    • 3
  1. 1.Department of Botany, Faculty of ScienceCharles University in PraguePrague 2Czech Republic
  2. 2.Laboratory of Genetics, Physiology and Bioengineering of FungiInstitute of Microbiology of the ASCR, v.v.i.Prague 4Czech Republic
  3. 3.Laboratory of Environmental MicrobiologyInstitute of Microbiology of the ASCR, v.v.i.Prague 4Czech Republic

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