Fungal Diversity

, Volume 52, Issue 1, pp 49–71

The sequestrate genus Rosbeeva T.Lebel & Orihara gen. nov. (Boletaceae) from Australasia and Japan: new species and new combinations



The sequestrate genus Chamonixia has been shown to have affinities to the Boletales, in particular the genus Leccinum. Australasian and Japanese species of Chamonixia were examined using morphological and molecular (ITS and nLSU rDNA) data and found to also have affinities with Leccinum and Leccinellum, however they form a distinct clade separate from the European type species C. caespitosa Rolland and North American species. A new genus, Rosbeeva T.Lebel & Orihara gen. nov., is proposed for the Australasian, Japanese and Chinese taxa. The species R. mucosa (Petri) T.Lebel comb. nov. is restricted in distribution to Singapore and Borneo, and R. pachyderma (Zeller & C.W. Dodge) T.Lebel comb. nov. to New Zealand, with Australian collections considered to belong to a revised R. vittatispora (G.W.Beaton, Pegler & T.W.K.Young) T.Lebel comb. nov. or a new species R. westraliensis T. Lebel sp. nov. The Chinese species R. bispora (B.C.Zhang & Y.N.Yu) T.Lebel & Orihara comb. nov is transferred to the new genus based upon morphological data. Two new species from Japan, Rosbeeva eucyanea Orihara and R. griseovelutina Orihara, are also described and illustrated. A key to all species of Rosbeeva is provided. Due to the highly modified gastroid sporocarp forms of both Chamonixia and Rosbeeva, many macroscopic characters of use in agaricoid taxonomy are difficult to interpret. However, color change and texture of sporocarps are of some use to distinguish genera and species. Microscopic characters such as spore shape, dimensions, and ornamentation, and pileipellis and hymenophoral trama structure, are essential for determining genera and species.


Chamonixia Sequestrate fungi Leccinum Systematics Octaviania 


  1. Beaton G, Pegler DN, Young TWK (1985) Gasteroid Basidiomycota of Victoria State, Australia: 5–7. Kew Bulletin 40:573–598CrossRefGoogle Scholar
  2. Binder M, Bresinsky A (2002) Derivation of a polymorphic lineage of Gasteromycetes from boletoid ancestors. Mycologia 94:85–98PubMedCrossRefGoogle Scholar
  3. Binder M, Hibbett DS (2006) Molecular systematics and biological diversification of Boletales. Mycologia 98:971–981PubMedCrossRefGoogle Scholar
  4. Bougher NL, Thiers HD (1991) An indigenous species of Leccinum (Boletaceae) from Australia. Mycotaxon XLII:255–262Google Scholar
  5. Corner EJ (1972) Boletus in Malaysia. Government Printing Office, Singapore, pp 102–107Google Scholar
  6. Corner HJH, Hawker LE (1953) Hypogeous fungi from Malaya. Trans Brit Mycol Soc 36:125–137CrossRefGoogle Scholar
  7. Cunningham GH (1979) The Gasteromycetes of Australia and New Zealand. Band 67. Cramer, VaduzGoogle Scholar
  8. Den Bakker HC, Noordeloos ME (2005) A revision of European species of Leccinum Gray and notes on extralimital species. Persoonia 18:511–587Google Scholar
  9. Desjardin DE, Wilson AW, Binder M (2008) Durianella, a new gasteroid genus of boletes from Malaysia. Mycologia 100:956–961PubMedCrossRefGoogle Scholar
  10. Faith DP (1992) Conservation evaluation and phylogenetic diversity. Biol Conserv 61:1–10CrossRefGoogle Scholar
  11. Faith DP, Baker AM (2006) Phylogenetic diversity (PD) and biodiversity conservation: some bioinformatics challenges. Bioinformatics 2:121–128Google Scholar
  12. Gardes M, Bruns TD (1993) ITS primers with enhanced specificity of basidiomycetes: application to the identification of mycorrhizae and rusts. Molecular Ecology 2, 113–118Google Scholar
  13. Giachini AJ, Hosaka K, Nouhra E, Spatafora JW, Trappe JM (2010) Phylogenetic relationships of the Gomphales based on nuc-25S-rDNA, mit-12S-rDNA, and mit-atp6-DNA combined sequences. Fungal Biol 114:224–234PubMedCrossRefGoogle Scholar
  14. Halling RE, Baroni TJ, Binder M (2007) A new genus of Boletaceae from eastern North America. Mycologia 99:310–316PubMedCrossRefGoogle Scholar
  15. Hongo T (1983) Notulae Mycologicae (18). Mem Fac Educ Shiga Univ Nat Sci 33:37–41Google Scholar
  16. Hosaka K, Bates ST, Beever RE, Castellano MA, Colgan W III, Dominguez LS, Geml J, Giachini AJ, Kenney SR, Nourha EA, Simpson NB, Trappe JM (2006) Molecular phylogenetics of the gomphoid-phalloid fungi with an establishment of the new sub-class Phallomycetidae and two new orders. Mycologia 98(6):949–959PubMedCrossRefGoogle Scholar
  17. Humpert AJ, Muench EL, Giachini AJ, Castellano MA, Spatafora JW (2001) Molecular phylogenetics of Ramaria and related genera: evidence from nuclear large subunit and mitochondrial small subunit rDNA sequences. Mycologia 93:466–477CrossRefGoogle Scholar
  18. Justo A, Morgenstern I, Hallen-Adams HE, Hibbett DS (2010) Convergent evolution of sequestrate forms in Amanita under Mediterranean climate conditions. Mycologia 102(3):675–88PubMedCrossRefGoogle Scholar
  19. Kretzer AM, Bruns DT (1999) Use of atp6 in fungal phylogenetics: an example from the Boletales. Molec Phylo Evol 13:483–492CrossRefGoogle Scholar
  20. Lebel T, Tonkin JE (2008) Australasian species of Macowanites are sequestrate species of Russula (Russulaceae, Basidiomycota). Aust Syst Bot 20(4):355–381CrossRefGoogle Scholar
  21. Maddison WP, Donoghue MJ, Maddison DR (1984) Outgroup analysis and parsimony. Systematic Zoology 33: 83-103Google Scholar
  22. Mleczko P, Kozak M, Lawrynowicz M, Gorszczyk A (2009) New localities of Chamonixia caespitosa (hypogeous Boletaceae) in Central Europe. Acta Mycologia 44:29–42Google Scholar
  23. Orihara T, Sawada F, Ikeda S, Yamato M, Tanaka C, Shimomura N, Hashiya M, Iwase K (2010) Taxonomic reconsideration of a sequestrate fungus, Octaviania columellifera, with the proposal of a new genus, Heliogaster, and its phylogenetic relationships in the Boletales. Mycologia 102:108–121PubMedCrossRefGoogle Scholar
  24. Peintner U, Bougher NL, Castellano MA, Moncalvo J-M, Moser MM, Trappe JM, Vilgalys R (2002) Multiple origins of sequestrate fungi related to Cortinarius (Cortinariaceae). Am J Bot 88(12):2168–2179CrossRefGoogle Scholar
  25. Rolland L (1899) Excursion a Chamonix- ete et automne 1898. Bull Soc Mycol Fr 15:73–78Google Scholar
  26. Sahr T, Ammer H, Besl H, Fischer M (1999) Infrageneric classification of the boleticolous genus Sepedonium: species delimitation and phylogenetic relationships. Mycologia 91(6): 935-943Google Scholar
  27. Smith AH, Singer R (1959) Studies on secotiaceous fungi- IV Gastroboletus, Truncocolumella and Chamonixia. Brittonia 11:205–223CrossRefGoogle Scholar
  28. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTALX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25: 4876-4882Google Scholar
  29. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In ‘PCR protocols: a guide to methods and applications’. (Eds MA Innis, DH Gelfand, JJ Shinsky, TJ White) pp. 315–322. (Academic Press: San Diego, CA)Google Scholar
  30. Vernes K, Lebel T (2011) Truffle consumption by New Guinea forest wallabies. Fungal Ecology. Fungal Ecology 4:270-276Google Scholar
  31. Yang ZL, Trappe JM, Binder M, Sanmee R, Lumyong P, Lumyong S (2006) The sequestrate genus Rhodactina (Basidiomycota, Boletales) in northern Thailand. Mycotaxon 96:133–140Google Scholar
  32. Zeller SM, Dodge CW (1934) Hymenogaster and related genera. Ann Miss Bot Gard 21:625–709CrossRefGoogle Scholar
  33. Zhang B-C, Yu Y-N (1989) Chamonixia bispora sp. nov. (Boletales) from China. Mycotaxon 35:277–281Google Scholar

Copyright information

© Kevin D. Hyde 2011

Authors and Affiliations

  • Teresa Lebel
    • 1
  • Takamichi Orihara
    • 2
    • 4
  • Nitaro Maekawa
    • 3
  1. 1.National Herbarium of Victoria, Royal Botanic Gardens MelbourneSouth YarraAustralia
  2. 2.The United Graduate School of Agriculture SciencesTottori UniversityTottoriJapan
  3. 3.Fungus/Mushroom Resource and Research Center, Faculty of AgricultureTottori UniversityTottoriJapan
  4. 4.Kanagawa Prefectural Museum of Natural HistoryKanagawaJapan

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