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Fungal Diversity

, Volume 49, Issue 1, pp 125–143 | Cite as

Zangia, a new genus of Boletaceae supported by molecular and morphological evidence

  • Yan Chun Li
  • Bang Feng
  • Zhu L. Yang
Article

Abstract

A new distinct genus of Boletales, Zangia, with phenotypic similarities to the genus Tylopilus, is proposed based on molecular and morphological data. The monophyly of Zangia was highly supported using two nuclear and three mitochondrial genes based on Maximum Parsimony, Maximum Likelihood and Bayesian analyses. Morphologically, Zangia is distinguished from other boletoid lineages by its combination of rugose pileus, pinkish to pink hymenophore, pink to pinkish brown spore deposit, pink scabrous squamules on the stipe, chrome yellow to golden yellow stipe base, chrome yellow to golden yellow mycelia on the base of the stipe, ixohyphoepithelium pileipellis, glabrous spores and bluish colour changes in the stipe in some species. Geographically, Zangia is currently only known from southern, southeastern and southwestern China under forests dominated by Fagaceae mixed with Pinaceae. Six species, including 4 new ones and 2 new combinations, are fully documented with taxonomic descriptions and illustrations. A key to the species in Zangia is provided. It is suggested that some of the species might have started diverging from each other relatively recently with the uplifts of the eastern Himalayas and Hengduan Mountains, and both the mycorrhizal host specificity or preference and geographic separation could contribute to their ongoing divergence.

Keywords

Boletes Mycorrhizal fungi Taxonomy Phylogeny Distribution 

Notes

Acknowledgments

The authors thank Dr. R. H. Petersen (TENN) for providing specimens on loan and granting permission to extract DNA from the type specimens. They are grateful to Dr. Z. W. Ge (HKAS) for providing collections, and to Dr. J. Xu (McMaster University) for polishing the English of the manuscript. Thanks are also due to Dr. R. Kirschner (Department of Life Sciences, National Central University) for improving the Latin. This study was supported by the Joint Funds of the National Natural Science Foundation of China and Yunnan Provincial Government (No. U0836604), the National Basic Research Program of China (No. 2009CB522300), the National Natural Science Foundation of China (No. 31000012), the Hundred Talents Program of the Chinese Academy of Sciences, the Ministry of Science and Technology of China (2008FY110300), the Research Fund for the Large-scale Scientific Facilities of the Chinese Academy of Sciences (2009-LSF-GBOWS-01) and the Knowledge Innovative Program of The Chinese Academy of Sciences (2010KIBB13).

References

  1. Binder M, Besl H (2000) 28S rDNA sequence data and chemotaxonomical analyses on the generic concept of Leccinum (Boletales). A.M.B., Italy. Centro Studi Micologici. Micologia 2000:71–82Google Scholar
  2. Binder M, Bresinsky A (2002a) Derivation of a polymorphic lineage of gasteromycetes from boletoid ancestors. Mycologia 94:85–98PubMedCrossRefGoogle Scholar
  3. Binder M, Bresinsky A (2002b) Retiboletus, a new genus for a species-complex in the Boletaceae producing retipolides. Feddes Repertorium 113:30–40CrossRefGoogle Scholar
  4. Binder M, Hibbett DS (2006) Molecular systematics and biological diversification of Boletales. Mycologia 98:971–981PubMedCrossRefGoogle Scholar
  5. Both EE (1993) The Boletes of North America: a compendium. Buffalo Museum of Science, Buffalo NY, p 436Google Scholar
  6. Bruns TD, Szaro TM (1992) Rate and mode differences between nuclear and mitochondrial small-subunit rRNA genes in mushrooms. Molecular Biology and Evolution 9:836–855PubMedGoogle Scholar
  7. Bruns TD, Szaro TM, Gardes M, Cullings KW, Pan JJ, Taylor DL, Horton TR, Kretzer A, Garbelotto M, Li Y (1998) A sequence database for the identification of ectomycorrhizal basidiomycetes by phylogenetic analysis. Molecular Ecology 7:257–272CrossRefGoogle Scholar
  8. Castellano MA, Trappe JM, Malajczuk N (1992) Australasian truffle-like fungi. III. Royoungia gen. nov. and Mycoamaranthus gen. nov. (Basidiomycotina). Australian Systematic Botany 5:613–616CrossRefGoogle Scholar
  9. Chiu WF (1948) The Boletes of Yunnan. Mycologia 40:200–231CrossRefGoogle Scholar
  10. Corner EJF (1972) Boletus in Malaysia. Government Printer, Singapore, pp 1–263Google Scholar
  11. den Bakker HC, Noordeloos ME (2005) A revision of European species of Leccinum Gray and notes on extralimital species. Persoonia 18:511–587Google Scholar
  12. Desjardin DE, Wilson AW, Binder M (2008) Durianella, a new gasteroid genus of boletes from Malaysia. Mycologia 100:956–961PubMedCrossRefGoogle Scholar
  13. Desjardin DE, Binder M, Roekring S, Flegel T (2009) Spongiforma, a new genus of gasteroid boletes from Thailand. Fungal Diversity 37:1–8Google Scholar
  14. Fu SZ, Wang QB, Yao YJ (2006) Tylopilus microsporus, a new species from southwest China. Mycotaxon 96:41–46Google Scholar
  15. Fulgenzi TD, Halling RE, Henkel TW (2010) Fistulinella cinereoalba sp. nov. and new distribution records for Austroboletus from Guyana. Mycologia 102:224–232PubMedCrossRefGoogle Scholar
  16. Gao Q, Yang ZL (2010) Ectomycorrhizal fungi associated with two species of Kobresia in an alpine meadow in the eastern Himalaya. Mycorrhiza 20:281–287PubMedCrossRefGoogle Scholar
  17. Gómez LD, Singer R (1984) Veloporphyrellus, a new genus of Boletaceae from Costa Rica. Brenesia 22:293–298Google Scholar
  18. Halling RE, Baroni TJ, Binder M (2007) A new genus of Boletaceae from eastern North America. Mycologia 99:310–316PubMedCrossRefGoogle Scholar
  19. Hongo T (1964) Notulae mycologicae (3). Memoirs of the faculty of liberal arts and education, Shiga University Natural Science 14: 43–47Google Scholar
  20. Huelsenbeck JP, Ronquist F (2005) Bayesian analysis of molecular evolution using MrBayes. In: Nielsen R (ed) Statistical methods in molecular evolution. Springer, New York, pp 183–232CrossRefGoogle Scholar
  21. Kornerup A, Wanscher JH (1981) Taschenlexikon der Farben. 3. Aufl. Göttingen: Muster-Schmidt VerlagGoogle Scholar
  22. Kretzer AM, Bruns TD (1999) Use of atp6 in fungal phylogenetics: an example from the Boletales. Molecular Phylogenetics and Evolution 13:483–492PubMedCrossRefGoogle Scholar
  23. Li TH, Song B (2003) Boletes species known from China. Guizhou Science 21(1–2):78–86Google Scholar
  24. Li TH, Song B, Shen YH (2002) A new species of Tylopilus from Guandong. Mycosystema 21:3–5Google Scholar
  25. Li YC, Yang ZL, Tolgor B (2009) Phylogenetic and biogeographic relationships of Chroogomphus species as inferred from molecular and morphological data. Fungal Diversity 38:85–104Google Scholar
  26. Matheny PB, Hibbett DS (2007) Contribution of rpb2 and tef1 to the phylogeny of mushrooms and allies (Basidiomycota, Fungi). Molecular Phylogenetics and Evolution 43:430–451PubMedCrossRefGoogle Scholar
  27. Mikheyev AS, Mueller UG, Abbot P (2006) Cryptic sex and many-to-one coevolution in the fungus-growing ant symbiosis. Proceedings of the National Academy of Sciences 103:10702–10706CrossRefGoogle Scholar
  28. Pegler DN, Young TWK (1981) A natural arrangement of the Boletales, with reference to spore morphology. Transactions of British Mycological Society 76:103–146CrossRefGoogle Scholar
  29. Peintner U, Ladurner H, Simonini G (2003) Xerocomus cisalpinus sp. nov., and the delimitation of species in the X. chrysenteron complex based on morphology and rDNA-LSU sequences. Mycological Research 107:659–679PubMedCrossRefGoogle Scholar
  30. Posada D, Buckley TR (2004) Model selection and model averaging in phylogenetics: advantages of the AIC and Bayesian approaches over likelihood ratio tests. Systematic Biology 53:793–808PubMedCrossRefGoogle Scholar
  31. Redeuilh G, Soop K (2006) Nomenclature et taxinomie des genres affines à Fistulinella (Boletaceae) et Fistulinella lutea sp. nov. de Nouvelle-Zélande. Bulletin trimestriel de la Société mycologique de France 122:291–304Google Scholar
  32. Singer R (1945) The Boletineae of Florida with notes on extralimital species. I. The Strobilomycetaceae. Farlowia 2:97–141Google Scholar
  33. Singer R (1947) The Boletoideae of Florida. The Boletineae of Florida with notes on extralimital species. III. American Midland Naturalist 37:1–135CrossRefGoogle Scholar
  34. Singer R (1986) Agaricales in modern taxonomy, 4th edn. Koeltz Scientific Books, KoenigsteinGoogle Scholar
  35. Smith AH, Thiers HD (1968) Notes on boletes-I 1. The generic position of Boletus subglabripes and Boletus chromapes 2. A comparison of four species of Tylopilus. Mycologia 60:943–954CrossRefGoogle Scholar
  36. Smith AH, Thiers HD (1971) The Boletes of Michigan. The University of Michigan Press, Ann ArborGoogle Scholar
  37. Snell WH, Dick EA (1970) The Boleti of Northeastern North America. Verlag von J Cramer, LehreGoogle Scholar
  38. Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web-servers. Systematic Biology 75:758–771CrossRefGoogle Scholar
  39. Swofford DL (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sunderland: Sinauer AssociatesGoogle Scholar
  40. Tai FL (1979) Sylloge fungorum sinicorum. Science Press, BeijingGoogle Scholar
  41. Thiers, B. (2010) [continuously updated]. Index herbariorum: a global directory of Public Herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. http://sweetgum.nybg.org/ih/
  42. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172:4238–4246PubMedGoogle Scholar
  43. Watling R, Gregory NM (1989) Observations on the boletes of the Cooloola Sandmass, Queensland and notes on their distribution in Australia. Part 2C. Smooth spored taxa - Strobilomycetaceae. Proceedings of the Royal Society of Queensland 100:13–30Google Scholar
  44. Watling R, Li TH (1999) Australian boletes, a preliminary survey. Royal Botanic Garden, Edinburgh, pp 1–71Google Scholar
  45. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, San Diego, pp 315–322Google Scholar
  46. Wolfe CB Jr (1979) Austroboletus and Tylopilus subgenus Porphyrellus with emphasis on North American taxa. Bibliotheca Mycologica 69:1–61Google Scholar
  47. Wolfe CB Jr, Bougher NL (1993) Systematics, mycogeography, and evolutionary history of Tylopilus subg. Roseoscabra in Australia elucidated by comparison with Asian and American species. Australian Systematic Botany 6:187–213CrossRefGoogle Scholar
  48. Wolfe CB Jr, Petersen RH (1978) Taxonomy and nomenclature of the supraspecific taxa of Porphyrellus. Mycotaxon 7:152–162Google Scholar
  49. Yang ZL (2005) Diversity and biogeography of higher fungi in China. In: Xu J (ed) Evolutionary genetics of fungi. Horizon Bioscience, Norfolk, pp 35–62Google Scholar
  50. Zang M (ed) (1996) Fungi of the Hengduan Mountains. Science, BeijingGoogle Scholar

Copyright information

© Kevin D. Hyde 2011

Authors and Affiliations

  1. 1.Key Laboratory of Biodiversity and BiogeographyKunming Institute of Botany, Chinese Academy of SciencesKunmingPeople’s Republic of China
  2. 2.Graduate University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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