Fungal Diversity

, Volume 49, Issue 1, pp 35–46 | Cite as

The contribution of tef-1 sequences to species delimitation in the Cantharellus cibarius complex in the southeastern USA

  • B. Buyck
  • Valérie Hofstetter


This study of the Cantharellus cibarius complex presents three new species for the southeastern USA based on recently collected material, as well as on revisions of type specimens and newly generated sequence data for the protein coding gene tef-1. Cantharellus lewisii sp. nov. is described for a southern species that was hitherto mistaken for the European ‘C. amethysteus’. C. altipes sp. nov. is introduced for a taxon that may correspond to C. cibarius var. longipes Peck. C. tenuithrix sp. nov. is described as the American sister taxon of the European C. cibarius s.s. The current confusing concept of C. lateritius, in contradiction with the original diagnosis, is here stabilized by the choice of a sequenced epitype. Its separation from C. confluens nom. inval. needs additional sampling. Being invalid, further use of the name C. confluens is discouraged.


Cantharellales Taxonomy Molecular systematics America Nomenclature Typification 



The curators of the mycological herbaria PH, NYS and TENN are acknowledged for loan facilities and efficiency. The sequencing for this study was performed by C. Cruaud and A. Couloux at the Genoscope or “Consortium National de Recherche en Génomique” near Paris (France) as part of the agreement n°2005/67 on the project “Macrophylogeny of life” (dir. G. Lecointre) between the Genoscope and the “service de systématique moléculaire” of the Muséum National d’Histoire Naturelle (CNRS IFR 101). David Lewis and family are thanked for generous hospitality and field assistance. Travel to the USA was made possible through several grants to the senior author by the Paris’ Museum Pluriformation program «Etat et structure phylogénétique de la biodiversité actuelle et fossile» (Dir. Ph. Janvier).

Supplementary material

13225_2011_95_MOESM1_ESM.doc (86 kb)
Online resource 1 Taxon sampling and Genbank accession numbers. Abbreviations used: C. = Cantharellus. Collection sources : BB = Bart Buyck; GE = Guillaume Eyssartier; AFTOL = “Assembling the Fungal Tree Of Life” consortium. (DOC 85 kb)


  1. Alfaro M, Zoller S, Lutzoni F (2003) Bayes or bootstrap? A simulation study comparing the performance of Bayesian Markov chain Monte Carlo sampling and bootstrapping in assessing phylogenetic confidence. Mol Biol Evol 20:255–266PubMedCrossRefGoogle Scholar
  2. Arora D, Dunham S (2009) A new, commercially valuable chanterelle species, Cantharellus californicus sp. nov. associated with Live Oak in California, USA. Econ Bot 62(3):376–391CrossRefGoogle Scholar
  3. Bigelow HE (1978) The Cantharelloid fungi of New England and adjacent areas. Mycologia 70(4):707–756CrossRefGoogle Scholar
  4. Buyck B (1994) Ubwoba. Les champignons comestibles de l’Ouest du Burundi. dans: Publications agricoles N° 34,123 pp., A.G.C.D., BruxellesGoogle Scholar
  5. Buyck B, Hofstetter V (2008) A multigene phylogeny for worldwide Cantharellus (MSA 2008 Abstracts). Inoculum 59(4):22Google Scholar
  6. Buyck B, Lewis DP, Eyssartier G, Hofstetter V (2010) Cantharellus quercophilus sp.nov. and its comparison to other small, yellow or brown American chanterelles. Cryptogam Mycol 31(1):17–33Google Scholar
  7. Buyck B, Cruaud C, Couloux A, Hofstetter V (2011) Cantharellus texensis sp. nov., a southern look-alike of C. cinnabarinus from Texas (USA) revealed by tef-1 sequence data. Mycologia (accepted)Google Scholar
  8. Corner EJH (1966) A monograph of Cantharelloid fungi. Ann Bot Mem 2, 255 pGoogle Scholar
  9. Dahlman M, Danell E, Spatafora JW (2000) Molecular systematics of Craterellus: cladistic analyses of nuclear LSU rDNA sequence data. Mycol Res 104(4):388–394Google Scholar
  10. Dancholia S, Bhatt JC, Pant SK (1991) Studies of some Himalaya agarics. Acta Bot Indica 19(1):104–109Google Scholar
  11. Dunham SN, O’Dell TE, Molina R (2003) Analysis of nrDNA sequences and microsatellite allele frequencies reveals a cryptic chanterelle species Cantharellus cascadensis sp. nov. from the American Pacific Northwest. Mycol Res 107(10):1163–1177PubMedCrossRefGoogle Scholar
  12. Eyssartier G (2001) Vers une monographie du genre Cantharellus Adans.:Fr. Dissertation. Museum national d’histoire naturelle. Paris. 259 ppGoogle Scholar
  13. Eyssartier G, Buyck B (2001a, for 2000) Le genre Cantharellus en Europe. Nomenclature et taxinomie. Bull Soc Mycol France 116(2):91–137Google Scholar
  14. Eyssartier G, Buyck B (2001b) Novitates. Note nomenclaturale et systématique sur le genre Cantharellus. Doc Mycol 31(121):55–56Google Scholar
  15. Eyssartier G, Stubbe D, Walleyn R, Verbeken A (2009) New records of Cantharellus species (Basidiomycota, Cantharellaceae) from Malaysian dipterocarp rainforest. Fungal Divers 36:57–67Google Scholar
  16. Feibelman T, Bayman P, Cibula WG (1994) Length variation in the internal transcribed spacer of ribosomal DNA in chanterelles. Mycol Res 98:614–618CrossRefGoogle Scholar
  17. Feibelman TP, Bennet JW, Cibula WG (1996) Cantharellus tabernensis: a new species from the southeastern United States. Mycologia 88(2):295–301CrossRefGoogle Scholar
  18. Heim R (1954) Observations sur le noyau des Basidiomycètes. Rev Mycol Paris 19:201–249, pl 2–4Google Scholar
  19. Heinemann P (1959) Cantharellineae. Flore Iconogr Champignons Congo 8:153–165, pl. 26–28Google Scholar
  20. Heinemann P (1966) Cantharellineae du Katanga. Bull Jard Bot État Brux 36:365–352Google Scholar
  21. Hermitte JC, Eyssartier G, Poumarat S (2005) Cantharellus lilacinopruinatus sp. nov., une nouvelle chanterelle termophile. Bull Fed Assoc Medit 28:27–32Google Scholar
  22. Hofstetter V, Clémençon H, Vilgalys R, Moncalvo JM (2002) Phylogenetic analyses of the Lyophylleae (Agaricales, Basidiomycetes) based on nuclear and mitochondrial rDNA sequences. Mycol Res 106(9):1043–1059CrossRefGoogle Scholar
  23. Hongo T, Izawa M (1994) [Fungi]. Yama-Kei Field Books, Yamakei publishers Co., Shiba-daimon, Minato-ku, Tokyo, Japan, 384 ppGoogle Scholar
  24. Kornerup A, Wanscher JP (1978) Methuen handbook of colour, 3rd edn. Eyre Methuen, LondonGoogle Scholar
  25. Kuo M (2006) Cantharellus lateritius. Retrieved from the web site ( as accessed on May 2010
  26. Lambotte JBE (1880) Flore Mycologique Belge 1. 524 ppGoogle Scholar
  27. Lutzoni F et al (2004) Where are we in assembling the fungal tree of life, classifying the fungi, and understanding the evolution of their subcellular traits. Am J Bot 91(10):1447–1480CrossRefGoogle Scholar
  28. Metzler S, Metzler V (1992) Texas mushrooms. A field guide. University of Texas Press, AustinGoogle Scholar
  29. Moncalvo JM et al (2007, for 2006) The cantharelloid clade: dealing with incongruent gene trees and phylogenetic reconstruction methods. Mycologia 98(6):937–948Google Scholar
  30. Morehouse EA, James TY, Ganley ARD, Vilgalys R, Berger L, Murphy PJ, Longcore JE (2003) Multilocus sequence typing suggests the chytrid pathogen of amphibians is a recently emerged clone. Mol Ecol 12:395–403PubMedCrossRefGoogle Scholar
  31. Oliaraga I, Salcedo I (2007) Cantharellus gallaecicus (Blanco-Dios) Olariaga, comb. & stat. nov. (Cantharellaceae). Anal Jard Bot Madr 64(2):221–222Google Scholar
  32. Oliaraga I, Salcedo I (2008) Cantharellus ilicis sp.nov., a new species from the Mediterranean basin collected in evergreen Quercus forests. Rev Catalana Micol 30:107–116Google Scholar
  33. Peck CH (1887) New York species of Cantharellus. New York State Mus Bull 1:34–43Google Scholar
  34. Peck CH (1903) Report of the state botanist 1902. New York State Mus Bull 67:3–160Google Scholar
  35. Petersen RH (1969) Notes on Cantharelloid fungi - II. Some new taxa, and notes on Pseudocraterellus. Persoonia 5:211–223Google Scholar
  36. Petersen RH (1976) Notes on the Cantharelloid fungi - VII. The taxa described by Charles H. Peck. Mycologia 68:304–326CrossRefGoogle Scholar
  37. Petersen RH (1979a) Notes on Cantharelloid fungi. IX. Illustrations of new or poorly understood taxa. Nova Hedwig 31:1–23Google Scholar
  38. Petersen RH (1979b) Notes on Cantharelloid fungi. X. Cantharellus confluens and C. lateritius, Craterellus odoratus and C. aureus. Sydowia 32:198–208Google Scholar
  39. Petersen RH (1986) Notes on Clavarioid fungi. XIX. Colored illustrations of selected taxa, with comments on Cantharellus. Nova Hedwig 42(1):151–160Google Scholar
  40. Petersen RH, Mueller G (1992) New south american taxa of Cantharellus, C. nothofagorum, C. xanthoscyphus and C. lateritius var. colombianus. Bol Soc Argent Bot 28:195–200Google Scholar
  41. Phillips R (1991) Mushrooms of North America. Little, Brown and Company, BostonGoogle Scholar
  42. Pilz D, Norvell L, Danell E, Molina R (2003) Ecology and management of commercially harvested chanterelle mushrooms. Gen Tech Rep PNW-GTR-576 Portland, OR. US Depart. Agric., Forest Service, Pacific Northwest Research StationGoogle Scholar
  43. Redhead SA, Norvell LL, Danell E (1997) Cantharellus formosus and the Pacific Golden Chanterelle harvest in Western North America. Mycotaxon 65:285–322Google Scholar
  44. Roody WC (2003) Mushrooms of West Virginia and the Central Appalachians. Kentucky Univ. Press, Lexington, p 520Google Scholar
  45. Sasia RF, Pérez-de-Gregorio MA, Eyssartier G (2003) Cantharellus parviluteus, une nouvelle espèce décrite de la Péninsule Ibérique. Bull Soc Mycol France 119(3–4):261–266Google Scholar
  46. Smith AH (1968) The Cantharellaceae of Michigan. Mich Bot 7:143–183Google Scholar
  47. Smith AH, Morse EE (1947) The genus Cantharellus in the western United States. Mycologia 39:497–534PubMedCrossRefGoogle Scholar
  48. Stamatakis A, Ludwig T, Meier H (2005) RAxML-III, a fast program for maximum likelihood-based inference of large phylogenetic trees. Bioinformatics 21:456–463PubMedCrossRefGoogle Scholar
  49. Tibuhwa D, Buyck B, Kivaisi A, Tibell L (2008) Cantharellus fistulosus sp.nov. from Tanzania. Cryptogam Mycol 29(2):129–135Google Scholar

Copyright information

© Kevin D. Hyde 2011

Authors and Affiliations

  1. 1.Département Systématique et évolution, UMR7205Muséum National d’Histoire NaturelleParisFrance
  2. 2.Department of plant protectionAgroscope Changins-Wädenswil Research Station ACWNyonSwitzerland

Personalised recommendations