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Fungal Diversity

, Volume 47, Issue 1, pp 85–95 | Cite as

Community composition of endophytic fungi in Acer truncatum and their role in decomposition

  • Xiang Sun
  • Liang-Dong GuoEmail author
  • K. D. Hyde
Article

Abstract

The mycota and decomposing potential of endophytic fungi associated with Acer truncatum, a common tree in northern China, were investigated. The colonization rate of endophytic fungi was significantly higher in twigs (77%) than in leaves (11%). However, there was no significant difference in the colonization rates of endophytic fungi between lamina (9%) and midrib (14%) tissues. A total of 58 endophytic taxa were recovered using two isolation methods and these were identified based on morphology and ITS sequence data. High numbers of leaf endophytes were obtained in the method to determine decomposition of leaves by the natural endophyte community (35 taxa) as compared to disk fragment methodology (9 taxa). The weight loss in A. truncatum leaves decomposed by endophyte communities increased with incubation time; the weight loss was significantly higher at 20 weeks than at 3 and 8 weeks. Both common and rare endophytic taxa produced extracellular enzymes in vitro and showed different leaf decay abilities. Our results indicated that the composition and diversity of endophytic fungi obtained differed using two isolation methods. This study suggests that endophytic fungi play an important role in recycling of nutrients in natural ecosystems.

Keywords

Endophyte Isolation method Leaf decomposition Extracellular enzymes 

Notes

Acknowledgements

This project is supported by the National Natural Science Foundation of China Grants (No. 30930005 and 30870087) and the Chinese Academy of Sciences Grant (No. KSCX2-YW-Z-0935).

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Copyright information

© Kevin D. Hyde 2010

Authors and Affiliations

  1. 1.Key Laboratory of Systematic Mycology & Lichenology, Institute of MicrobiologyChinese Academy of SciencesBeijingChina
  2. 2.School of ScienceMae Fah Luang UniversityChiang RaiThailand

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