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Fungal Diversity

, Volume 41, Issue 1, pp 115–123 | Cite as

Endophytic mycobiota of leaves and roots of the grass Holcus lanatus

  • S. Sánchez Márquez
  • G. F. Bills
  • L. Domínguez Acuña
  • I. Zabalgogeazcoa
Article

Abstract

Holcus lanatus is a grass that grows in humid, often waterlogged soils in temperate zones around the world. The purpose of this work was to identify fungal endophytes associated with its roots and leaves, and to describe the diversity and spatial distribution patterns found in its mycobiota. Holcus plants were sampled at 11 locations in western and northern Spain, and endophytes were isolated from leaves and roots of each plant. Morphological and molecular methods based on the ITS1-5.8SrRNA-ITS2 sequence were used for isolate identification. In total, 134 different species were identified, 77 occurred in leaves, 79 in roots, and 22 were common to both organs. The dominant species of the mycobiota were isolated from roots and leaves, and were species generally considered as multi-host endophytes. The species richness was similar in leaves and roots, but the composition of isolates from roots varied more among locations than in leaf mycobiotas, suggesting that soil characteristics may have strongly influenced the root mycobiota. Significant variations with respect to the composition of their mycobiota among different locations indicate that beta diversity is a first order factor governing the richness and distribution of the endophytic mycobiota in grasses.

Keywords

Endophytes Poaceae Grasses Taxonomy Diversity 

Notes

Acknowledgements

Part of this research was financed with research projects GR64 and CSI04A07, granted by the Junta de Castilla y León.

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Copyright information

© Kevin D Hyde 2010

Authors and Affiliations

  • S. Sánchez Márquez
    • 1
  • G. F. Bills
    • 2
  • L. Domínguez Acuña
    • 1
  • I. Zabalgogeazcoa
    • 1
  1. 1.Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA-CSIC)SalamancaSpain
  2. 2.Fundación Medina, Parque Tecnológico Ciencias de la SaludGranadaSpain

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