Advertisement

Fungal Diversity

, Volume 40, Issue 1, pp 103–117 | Cite as

New Ceratocystis species infecting coffee, cacao, citrus and native trees in Colombia

  • M. Van WykEmail author
  • B. D. Wingfield
  • M. Marin
  • M. J. Wingfield
Article

Abstract

Ceratocystis fimbriata sensu lato includes a large number of plant and especially tree pathogens. In Colombia, isolates of this fungus cause a serious canker-stain disease on coffee as well as other fruit trees. Large collections of these isolates have been shown to occur in two distinct phylogenetic lineages based on ITS sequence comparisons. The aim of this study was to compare representatives of these two groups of isolates from coffee, citrus, cacao and native trees in Colombia, based on morphology and DNA-sequences for three gene regions. Host-specificity of the fungus was also considered. Representatives of the two groups of isolates were morphologically distinct and could be distinguished based on DNA sequence comparisons. They are also distinct from other species in the C. fimbriata sensu lato species complex and the sweet potato pathogen C. fimbriata sensu stricto and are provided with the names C. colombiana sp. nov and C. papillata sp. nov. There was no evidence for host-specificity amongst isolates of these two fungi that collectively represent a serious threat to coffee production in Colombia.

Keywords

Ophiostomatoid fungi Phylogenetic relationships Species concepts Tree diseases 

Notes

Acknowledgements

We thank the National Research Foundation (NRF), members of the Tree Protection Co-operative Programme (TPCP), the THRIP initiative of the Department of Trade and Industry and the Department of Science and Technology (DST)/NRF Centre of Excellence in Tree Health Biotechnology (CTHB) for funding. We also thank Dr. Hugh Glen for providing the Latin descriptions and for suggesting names for the new species. The assistance of Prof. Bernard Slippers in the analysing of some of the data is also gratefully acknowledged.

References

  1. Alves A, Crous PW, Correia A, Phillips AJL (2008) Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Divers 28:1–13Google Scholar
  2. Barnes I, Gaur A, Burgess T, Roux J, Wingfield BD, Wingfield MJ (2001) Microsatellite markers reflect intra-specific relationships between isolates of the vascular wilt pathogen, Ceratocystis fimbriata. Mol Plant Pathol 2:319–325CrossRefGoogle Scholar
  3. Castaño J (1951) Interpretatión de los síntomas y los signos de la enfermedad de la macana en café para el establecimiento de la diagnosis. Cenicafé 2:7–32Google Scholar
  4. Clement M, Posada D, Crandall K (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1660CrossRefPubMedGoogle Scholar
  5. Crous PW (2009) Taxonomy and phylogeny of the genus Mycosphaerella and its anamorphs. Fungal Divers 38:1–24Google Scholar
  6. Cunningham CW (1997) Can three incongruence tests predict when data should be combined? Mol Biol Evol 14:733–740PubMedGoogle Scholar
  7. Engelbrecht CJ, Harrington TC (2005) Intersterility, morphology and taxonomy of Ceratocystis fimbriata on sweet potato, cacao and sycamore. Mycologia 97:57–69CrossRefPubMedGoogle Scholar
  8. Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous Ascomycetes. Appl Environ Microbiol 61:1323–1330PubMedGoogle Scholar
  9. Halsted BD (1890) Some fungous disease of the sweet potato. Agricultural College Experiment Station Bulletin 76:1–32Google Scholar
  10. Harrington TC (2000) Host specialization and speciation in the American wilt pathogen Ceratocystis fimbriata. Fitopatol Bras 25:262–263Google Scholar
  11. Jacobs K, Bergdahl DR, Wingfield MJ, Halik S, Seifert KA, Bright DE, Wingfield BD (2004) Leptographium wingfieldii introduced into North America and found associated with exotic Tomicus piniperda and native bark beetles. Mycol Res 108:411–418CrossRefPubMedGoogle Scholar
  12. Johnson JA, Harrington TC, Engelbrecht CJB (2005) Phylogeny and taxonomy of the North American clade of the Ceratocystis fimbriata complex. Mycologia 97:1067–1092CrossRefPubMedGoogle Scholar
  13. Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30:3059–3066CrossRefPubMedGoogle Scholar
  14. Kile GA (1993) In: Wingfield MJ, Seifert KA, Webber JA (eds) Ceratocystis and ophiostoma: taxonomy, ecology and pathogenicity. APS, St. Paul, pp 173–183Google Scholar
  15. Kvas M, Marasas WFO, Wingfield BD, Wingfield MJ, Steenkamp ET (2009) Diversity and evolution of Fusarium species in the Gibberella fujikuroi complex. Fungal Divers 34:1–21Google Scholar
  16. Lazzizera C, Frisullo S, Alves A, Lopes J, Phillips AJL (2008) Phylogeny and morphology of Diplodia species on olives in southern Italy and description of Diplodia olivarum sp. nov. Fungal Divers 31:63–71Google Scholar
  17. Marin M (2004) Phylogenetic and molecular population biology studies on Ceratocystis spp. associated with conifer and coffee diseases. PhD thesis, Department of Plant pathology and Microbiology, University of Pretoria, South AfricaGoogle Scholar
  18. Marin M, Castro B, Gaitan A, Preisig O, Wingfield BD, Wingfield MJ (2003) Relationships of Ceratocystis fimbriata isolates from Colombian coffee-growing regions based on molecular data and pathogenicity. J Phytopathol 151:395–405CrossRefGoogle Scholar
  19. Mourichon X (1994) Serious Citrus dieback in Colombia caused by Ceratocystis fimbriata. Fruits 49:415–416Google Scholar
  20. Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala UniversityGoogle Scholar
  21. Pardo-Cardona VM (1995) Hongos fitopatógenos de Colombia. Medellín, Colombia. Dissertation, Universidad Nacional de ColombiaGoogle Scholar
  22. Pontis RE (1951) A canker disease of the coffee tree in Colombia and Venezuela. Phytopathology 41:179–184Google Scholar
  23. Prihastuti H, Cai L, Chen H, Hyde KD (2009) Characterization of Colletotrichum species associated with coffee berries in Chiang Mai, Thailand. Fungal Diversity 39:89–109Google Scholar
  24. Rayner RW (1970) A mycological colour chart. Commonwealth Mycological Institute and British Mycological Society, KewGoogle Scholar
  25. Rodas C, Roux J, Van Wyk M, Wingfield BD, Wingfield MJ (2007) Ceratocystis neglecta sp. nov., infecting Eucalyptus trees in Colombia. Fungal Divers 28:73–84Google Scholar
  26. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574CrossRefPubMedGoogle Scholar
  27. Rossetto CJ, Ribeiro IJA (1990) Mango wilt. XII. Recommendations for control. Revista de Agricultura Piracicaba 65:173–180Google Scholar
  28. Roux J, Heath RN, Labuschagne L, Nkuekam GK, Wingfield MJ (2007) Occurrence of the wattle wilt pathogen, Ceratocystis albifundus on native South African trees. Forest Pathol 37:1–11CrossRefGoogle Scholar
  29. Santos JM, Phillips AJL (2009) Resolving the complex of Diaporthe (Phomopsis) species occurring on Foeniculum vulgare in Portugal. Fungal Divers 34:111–125Google Scholar
  30. Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (* and other methods). Version: 4. Sinauer, SunderlandGoogle Scholar
  31. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599CrossRefPubMedGoogle Scholar
  32. Van Wyk M, Roux J, Barnes I, Wingfield BD, Liew ECY, Assa B, Summerell BA, Wingfield MJ (2004) Ceratocystis polychroma sp. nov., a new species from Syzygium aromaticum in Sulawesi. Stud Mycol 50:273–282Google Scholar
  33. Van Wyk M, Van der Merwe NA, Roux J, Wingfield BD, Kamgan GN, Wingfield MJ (2006) Population genetic analyses suggest that the Eucalyptus fungal pathogen Ceratocystis fimbriata has been introduced into South Africa. S Afr J Sci 102:259–263Google Scholar
  34. Van Wyk M, Al Adawi AO, Khan IA, Deadman ML, Al Jahwari A, Wingfield BD, Ploetz RC, Wingfield MJ (2007a) Ceratocystis manginecans sp. nov., causal disease of a destructive mango wilt disease in Oman and Pakistan. Fungal Divers 27:213–230Google Scholar
  35. Van Wyk M, Pegg G, Lawson S, Wingfield MJ (2007b) Ceratocystis atrox sp. nov associated with Phoracantha acanthocera infestations on Eucalyptus in Australia. Australas Plant Pathol 36:407–414CrossRefGoogle Scholar
  36. Van Wyk M, Wingfield BD, Mohali S, Wingfield MJ (2009) Ceratocystis fimbriatomima, a new species in the C. fimbriata sensu lato complex isolated from Eucalyptus trees in Venezuela. Fungal Divers 34:173–183Google Scholar
  37. Webster R, Butler E (1967) A morphological and biological concept of the species Ceratocystis fimbriata. Can J Bot 45:1457–1468CrossRefGoogle Scholar
  38. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a sequencing guide to methods and applications. Academic, San Diego, pp 315–322Google Scholar
  39. Yang YL, Liu ZY, Cai L, Hyde KD, Yu ZN, Mckenzie EHC (2009) Collectrotrichum anthracnose of Amaryllidaceae. Fungal Divers 39:123–146Google Scholar
  40. Zimmerman A (1900) Ueber den Krebs von Coffea arabica, verursacht Rostrella coffeae gen. et sp.n. Bulletin of the Institute of Botanical Gardens Buitenzorg 4:19–22Google Scholar

Copyright information

© Kevin D Hyde 2010

Authors and Affiliations

  • M. Van Wyk
    • 1
    Email author
  • B. D. Wingfield
    • 1
  • M. Marin
    • 2
  • M. J. Wingfield
    • 1
  1. 1.Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  2. 2.National University of ColombiaMedellinColombia

Personalised recommendations