Selection of potentially probiotic Kluyveromyces lactis for the fermentation of cheese whey–based beverage

  • Daelen Resende Oliveira
  • Ana Claudia Alencar Lopes
  • Rafaela Andrade Pereira
  • Patricia Gomes Cardoso
  • Whasley Ferreira DuarteEmail author
Original Article



This work aimed to assess the probiotic potential of different Kluyveromyces lactis strains isolated from Canastra cheese and to produce a fermented cheese whey beverage added to beetroot juice using the selected strain.


Kluyveromyces lactis strains were tested for their resistance to the passage through the simulated gastrointestinal tract, adhesion properties, and functional effects such as inhibition of enteric pathogens, short-chain fatty acids (SCFA) production, and β-galactosidase activity. The selected strain was used to produce a fermented cheese whey beverage added to beetroot juice in different proportions. The produced beverages were characterized using HPLC for sugars, Folin-Ciocalteu for total phenolic content, DPPH for antioxidant activity, and GC-MS for volatiles compounds.


Except B51, all strains showed viability above 75% after exposure to the simulated gastric and duodenal juices. The aggregation rates were above 84% in 24 h. Only B9 and C16 strains presented hydrophobicity above 60%. The highest B9 β-galactosidase activities were 2.17 U/g and 2.21 U/g for pH 7 and 9, respectively. The B9 SCFA profile was similar to that found for Saccharomyces bourllardi. The fermented cheese whey beverages presented phenolic content ranging from 102.75 to 291.61 μg EAG/mL and inhibition of DPPH ranging from 38.69 to 81.02% after 21 days of storage, besides being lactose free. Esters and acetates were the most abundant compounds.


Kluyveromyces lactis B9 presented interesting results as a potential probiotic yeast. The produced beverages allowed the delivery of K. lactis B9 through innovative product with functional properties.


β-Galactosidase Functional beverages Antioxidant activity Volatile compounds 


Funding information

This study was financially supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, Conselho Nacional de Desenvolvimento Científico e Tecnológico do Brasil (CNPq), and Fundação de Amparo à Pesquisa de MG (FAPEMIG).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This study did not involve human participants or animals.

Informed consent

The authors mutually agree with the submission of the manuscript to Annals of Microbiology.


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Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  1. 1.Department of BiologyUniversity of Lavras (UFLA)LavrasBrazil

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