First outbreak reported caused by Erysipelothrix species strain 2 in turkeys from poultry-producing farms in Brazil
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The aims of this study were to report the first isolation of Erysipelothrix sp. strain 2 (ES2) associated with clinical signs of diseases as well as mortality in turkeys and identify the antimicrobial resistance of the isolates.
We evaluated 118 farms for bacteriological analysis and TaqMan real-time PCR to identify the microorganism in different organs. After this, we made the epidemiological analysis between the positive flocks and the mortality mean. We performed the sequencing of the 16S rRNA region and the assessment of antimicrobial resistance.
We have identified 18 (15.25%) as ES2-positive flocks, without any other species from the same genus being found. After analysing the organ samples, we found liver as the organ of choice for the isolation of the ES2. The sequencing of 16S rRNA region of ES2 identified high homology with E. tonsillarum and E. rhusiopathiae, suggesting that it is not the best-suited target to identify this species. We have found a positive association between isolation of the bacteria in organs and flocks’ mortality. Positive flocks had a mortality mean rate of 6.87%, which is significantly greater than 3.76% in negative flocks. Ill turkeys had gross lesions of generalized septicaemia. The bacterial isolates showed high resistance to fosfomycin and trimethoprim/sulfamethoxazole and sensibility to norfloxacin, amoxicillin and lincomycin/spectinomycin.
This is the first study in the world that addressed ES2 as the causative agent of erysipelas in turkey.
KeywordsPoultry Erysipelas Septicaemia 16S rRNA region sequencing Antimicrobial resistance
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for their financial support.
Compliance with ethical standards
Conflicts of interest
The authors declare that they have no conflict of interests.
Research involving human animals
The necropsied birds of this work were naturally and recently dead, that by Brazilian Law No. 11,794 of October 8, 2008, there is no need for an evaluation by the Ethics Committee in animal research (because it deals with the handling of dead animals). Even so, we have communicated the Committee on Ethics in the Use of Animals of the Federal University of Uberlandia that certified our work with the number A004/19.
- Balootaki PA, Amin M, Haghparasti F, Rokhbakhsh-Zamin F (2017) Isolation and detection of Erysipelothrix rhusiopathiae and its distribution in humans and animals by phenotypical and molecular methods in Ahvaz-Iran in 2015. Iran J Med Sci 42:377–383Google Scholar
- Bricker JM, Saif YM (2013) Erysipelas. In: Swayne DE, Glisson JR, LR MD, Nolan LK, Suarez LD, Nair V (eds) Diseases of poultry. Iowa State University Press, Ames, pp 812–826Google Scholar
- Chooromoney KN, Hampson DI, Eamens GJ, Turner MJ (1994) Analysis of E.rhusiopathiae and E.tonsillarum by multilocus enzyme electrophoresis. J Clin Microbiol 32:371–376Google Scholar
- Schmitt F, Schade B, Böhm B, Shimoji Y, Pfahler C (2014) Erysipelas in a free-range flock with conjunctival oedema as an unusual clinical sign. Berl Munch Tierarztl Wochenschr 127:183–187Google Scholar
- Takeshi K, Makino S, Ikeda T, Takada N, Nakashiro A, Nakanishi K, Oguma K, Katoh Y, Sunagawa H, Ohyama T (1999) Direct and rapid detection by PCR of Erysipelothrix sp. DNAs prepared from bacterial strains and animal tissues. J Clin Microbiol 37:4093–4098Google Scholar
- Zavalla LD, Swayne DE, Glisson JR, Pearson JE, Reed WM, Jackwood MW (2008) A laboratory manual for the isolation, identification and characterisation of avian pathogens. American association of avian Pathologistis, Washington, D.C, 3:10–11Google Scholar