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Probiotic properties of Lactobacillus strains with high cinnamoyl esterase activity isolated from jeot-gal, a high-salt fermented seafood

  • Jong-Hui Kim
  • Sang-Ho BaikEmail author
Original Article
  • 13 Downloads

Abstract

Cinnamoyl esterases (CEs) improve the bioavailability of caffeic acid, a potent antioxidant with beneficial health effects. This study aimed to characterize the probiotic properties of 14 strains of CE-producing lactic acid bacteria (LAB) isolated from jeot-gal, a high-salt fermented seafood. We evaluated properties of the probiotic LAB with high CE activity, including tolerance to low pH and bile salts, antimicrobial activity, surface hydrophobicity, adhesion, and immunomodulatory effects, in vitro. All LAB tested tolerated pH 2.0 and 3% Oxgall, i.e., conditions comparable with those in the gastrointestinal environment. Three isolates, Lactobacillus paracasei JBCC10650, Lactobacillus pentosus JBCC10659, and Lactobacillus plantarum JBCC10543, showed stronger adherence to epithelial cells (12.3, 9.6, and 9.4%) than a commercial probiotic Lactobacillus rhamnosus GG (9.1%; p < 0.05), and exhibited broad antibacterial activity against putative pathogens. Most of the 14 LAB strains were able to regulate mRNA expression of pro- and anti-inflammatory cytokines in macrophages, indicating their potential immunomodulatory effects. Our findings suggest that the newly isolated CE-producing probiotics may show beneficial health effects by supporting the host immune system.

Keywords

Adhesion Cinnamoyl esterase Cytokine Lactobacillus Probiotics 

Abbreviations

CA

Caffeic acid

CE

Cinnamoyl esterase

ChlA

Chlorogenic acid

LAB

Lactic acid bacteria

Notes

Funding information

This research was supported by the Ministry of Trade, Industry & Energy (MOTIE), Korea Institute for Advancement of Technology (KIAT) and Establishment of Infrastructure for Industrialization of Korean Useful Microbes (R0004073).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

None.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

13213_2018_1424_MOESM1_ESM.docx (20 kb)
Table S1 (DOCX 19 kb)
13213_2018_1424_MOESM2_ESM.docx (17 kb)
Table S2 (DOCX 17 kb)

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Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  1. 1.Department of Food Science and Human NutritionChonbuk National UniversityJeonjuRepublic of Korea
  2. 2.Rural Development AdministrationNational Institutes of Animal ScienceWanjuRepublic of Korea

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