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Effect of organic carbon sources and environmental factors on cell growth and lipid content of Pavlova lutheri

  • Khawaja Muhammad Imran Bashir
  • Sana Mansoor
  • Na-Ri Kim
  • Florian Rolando Grohmann
  • Abid Ali Shah
  • Man-Gi ChoEmail author
Original Article
  • 27 Downloads

Abstract

The present study aimed to investigate the effects of organic carbon sources, cultivation methods, and environmental factors on growth and lipid content of Pavlova lutheri for biodiesel production. In the 250-mL flask bioreactors, P. lutheri was cultivated in the modified artificial seawater (ASW) medium containing glucose, glycerol, sodium acetate, or sucrose as an organic carbon substrate. The effects of different growth conditions (phototrophic, mixotrophic, and heterotrophic) and environmental factors such as photoperiod, light intensity, and salinity were evaluated. Growth of P. lutheri was inhibited under heterotrophy but was enhanced in mixotrophy as compared to phototrophy. Biomass and lipid content of P. lutheri were significantly (p < 0.05) affected by changing photoperiod, light intensity, and salinity. Higher biomass concentration and lipid content were observed at a light intensity of 100 ± 2 μmol photons m−2 s−1, 18 h photoperiod, and 30% salinity, in a modified ASW medium supplemented with 10 mmol sucrose. An increase in biomass concentration from 320 ± 25.53 to 1106 ± 18.52 mg L−1 and high lipid content of 31.11 ± 1.65% (w/w) were observed with the optimized culture conditions, demonstrating a significant (p < 0.05) enhancement in biomass and lipid content due to the improved culture conditions. The present study emphasizes the possible use of sucrose for biomass and lipid production with P. lutheri under the optimized culture conditions. Using low-cost and relatively easy accessible feedstock such as sucrose would be a valuable alternative for growing microalgae with enhanced lipid content.

Keywords

Biomass Growth optimization Growth kinetics Haptophyte Lipid content Pavlova lutheri 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

Not applicable.

Informed consent

Not applicable.

Ethical approval

Not applicable.

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Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  1. 1.Department of Biotechnology, Division of Energy and BioengineeringDongseo UniversityBusanRepublic of Korea
  2. 2.Research Center for Extremophiles and Microbiology, College of Medical and Life SciencesSilla UniversityBusanRepublic of Korea
  3. 3.Department of Environmental Engineering, College of EngineeringDong-A UniversityBusanRepublic of Korea
  4. 4.Institute of BiotechnologyTechnische Universität BerlinBerlinGermany

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