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Oxidative enzymes activity and hydrogen peroxide production in white-rot fungi and soil-borne micromycetes co-cultures

  • Chan-Cupul WiberthEmail author
  • Arámbula-Zúñiga Citlalli Casandra
  • Fan Zhiliang
  • Heredia Gabriela
Original Article
  • 58 Downloads

Abstract

Fungal co-cultures appear to be advantageous for ligninolytic enzyme (LE) production compared to single fungal strains. The aims of this study were (1) to determine the type of fungal interactions in the co-cultures of two white-rot fungi (WRF, Pycnoporus sanguineus and Trametes maxima) and eight soil-borne micromycetes (SBM), (2) to determine the laccase and manganese peroxidase (MnP) activities and the hydrogen peroxide (H2O2) production in two compatible fungal and micromycetic co-cultures in submerged fermentation, and (3) to understand the effect of H2O2 on LE production by WRF through a dose-response bioassay. In the co-culture of SBM and Pycnoporus sanguineus, the main interaction was deadlock at a distance, whereas T. maxima showed competitive antagonism and replaced the SBM. In the agar plates, Purpureocillium lilacinum (27.8-fold increase) and Beauveria brongniartii (9.4-fold increase) enhanced the laccase and MnP activities of P. sanguineus, and Metarhizium anisopliae (Ma129) (0.83-fold increase) and Trichoderma sp. SP6 (22.6-fold increase) similarly enhanced these activities in T. maxima. In submerged fermentation, P. lilacinum also increased the laccase and MnP activities of P. sanguineus. The laccase activity of T. maxima only increased in the co-culture with B. brongniartii. The co-cultures achieved higher H2O2 production compared to the WRF monoculture, which played a vital role in the increase of LE. The dose-response assays revealed that low concentrations of H2O2 (2.94 and 14.69 mM) enhance the laccase and MnP activities in WRF.

Keywords

Fungal co-culture Hydrogen peroxide production Laccase Manganese peroxidase 

Notes

Funding information

The authors are grateful for the funding of the project “Mechanism of increasing ligninolytic enzyme activities of fungal co-cultures between white rot fungi and soil borne micromycetes” by the Institute Mexico-United States of the California University (UC-MEXUS-CONACyT).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Università degli studi di Milano 2018

Authors and Affiliations

  • Chan-Cupul Wiberth
    • 1
    Email author
  • Arámbula-Zúñiga Citlalli Casandra
    • 1
  • Fan Zhiliang
    • 2
  • Heredia Gabriela
    • 3
  1. 1.Biological Control and Applied Mycology Laboratory, Faculty of Biological and Agro-Livestock SciencesUniversity of ColimaTecomanMexico
  2. 2.Department of Biological and Agricultural EngineeringUniversity of California DavisDavisUSA
  3. 3.Micromycetes LaboratoryInstitute of Ecology (INECOL A. C.)XalapaMexico

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