Annals of Microbiology

, Volume 68, Issue 12, pp 953–962 | Cite as

Assessment of atrazine decontamination by epiphytic root bacteria isolated from emergent hydrophytes

  • Anina James
  • Dileep Kumar SinghEmail author
Original Article


Aim of the study was to identify atrazine remediating bacteria that can potentially succeed in situ where they encounter varied environmental conditions. Three epiphytic root bacteria, genus Pseudomonas and Arthrobacter, were isolated from rhizoplanes of hydrophytes Acorus calamus, Typha latifolia, and Phragmites karka. Potential of these strains to decontaminate environmentally relevant concentrations of atrazine was determined in liquid atrazine medium (LAM) and Luria-Bertani (LB) medium at varying pH and temperature. There was an increase in decontamination by the strains with time upon exposure to 2.5 to 10 mg l−1 atrazine over a period of 15 days, notably, in both minimal and nutrient-rich media. Growth in terms of O.D.600 and biomass determined during the same period also showed a corresponding surge. Pseudomonas sp. strain AACB mitigated atrazine in a wide range of pH (5 to 8). Pseudomonas sp. strains AACB and TTLB decontaminated > 62% atrazine at 10 °C. All the strains exhibited plant growth–promoting traits in vitro, reported for the first time in the presence of atrazine. Strain AACB exhibits the novel trait of atrazine decontamination under harsh environmental conditions mimicked in lab. Strains isolated in the present study promise success in in situ remediation. Bioreactors and water treatment plants can be designed comprising the hydrophytes and the strains inoculated into their rhizospheres to improve efficacy of the treatment. They can be used to study plant-bacterium mutualistic symbiosis or other interactions occurring during atrazine mitigation.


Arthrobacter Bioremediation Plant growth–promoting rhizobacteria (PGPR) Pseudomonas Rhizoremediation 



I am thankful to Mr. Souvik Sen Sharma, Cellular Endocrinology Lab, National Institute of Immunology, for his valuable input in data analysis and comments regarding the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13213_2018_1404_MOESM1_ESM.docx (4.9 mb)
ESM 1 (DOCX 5033 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan 2018

Authors and Affiliations

  1. 1.Soil Microbial Ecology and Environmental Toxicology Laboratory, Department of ZoologyUniversity of DelhiDelhiIndia

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