Annals of Microbiology

, Volume 68, Issue 12, pp 889–897 | Cite as

Interstrain transfer of the prophage ϕNM2 in staphylococcal strains

  • Bing Yan
  • Yiming Pan
  • Zeyu Jin
  • Xiaoyu Liu
  • Wei Li
  • Baolin SunEmail author
Original Article


Staphylococcus aureus is a successful pathogen in part because the bacterium can adapt rapidly to selective pressures imparted by the external environment. Horizontal gene transfer (HGT) plays an integral role in the evolution of bacterial genomes, and phage transduction is likely to be the most common and important HGT mechanism for S. aureus. Phage can transfer not only its own genome DNA but also host bacterial DNA with or without pathogenicity islands to other bacteria. Here, we demonstrate that the staphylococcal prophage ϕNM2 could transfer between strains Newman and NCTC8325/NCTC8325-4 by simulating a natural situation in laboratory without mitomycin C or ultra-violet light treatment. This transference may be caused by direct contact between Newman and NCTC8325/NCTC8325-4 instead of phage particles released in Newman culture’s supernatant. The rates of successful horizontal genetic transfer in recipients NCTC8325 and NCTC8325-4 were 2.1% and 1.8%, respectively. Prophage ϕNM2 was integrated with one direction at an intergenic region between rpmF and isdB in all 17 lysogenic isolates. Phage particles were spontaneously released from lysogenic strains again and had no noticeable influence on the growth of host cells. The results reported herein provide insight into how mobile genetic elements such as prophages can lead to the emergence of genetic diversity among S. aureus strains.


Staphylococcus aureus Prophage ϕNM2 Interstrain transfer NCTC8325/NCTC8325-4 Newman 



We thank Professor M Li at Shanghai Jiaotong University for providing the clinical strains of Staphylococcus aureus.

Funding information

This study was supported in part by Mudanjiang Normal University (SY201230).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan 2018

Authors and Affiliations

  1. 1.School of Life SciencesMudanjiang Normal UniversityMudanjiangChina
  2. 2.School of Life SciencesUniversity of Science and Technology of ChinaHefeiChina

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