Annals of Microbiology

, Volume 68, Issue 12, pp 851–861 | Cite as

Isolation and culturing of protease- and lipase-producing Halococcus agarilyticus GUGFAWS-3 from marine Haliclona sp. inhabiting the rocky intertidal region of Anjuna in Goa, India

  • Sanket Krishnanath Gaonkar
  • Irene Jeronimo FurtadoEmail author
Original Article


Three extremely halophilic bionts GUGFAWS-1, GUGFAWS-2, and GUGFAWS-3 were isolated from a marine, white sponge, attached to rocks, in the intertidal region of Anjuna, Goa, India (15° 34′ 05″ N, 73° 44′ 17° 40′ E). Because the sponge had irregular tubes arranged in clusters, it was identified as Haliclona sp. All sponge bionts produced protease and lipase. GUGFAWS-1 and GUGFAWS-2 were euryhaline Eubacteria, growing from 0 to 30% NaCl concentration. The biont, GUGFAWS-3, was a haloarchaeon having glycerol diether moieties in its cells and showed R-O-R and long isoprenoid chains, in FTIR. The haloarchaeon, GUGFAWS-3, simultaneously produced extracellular 49.5 U mL−1 of protease and 3.67 U mL−1 of lipase, in the presence of 25% NaCl. It grew as dark orange-red colonies at 5–30% NaCl. Its growth was sensitive to bile salts and resistant to 700 U of penicillin. Cells were Gram-negative cocci, arranged in pairs, and 1-μm size in SEM micrograph. It possessed bacterioruberin with absorption at 387, 468, 492, and 523 nm. The 16S rRNA gene sequence of GUGFAWS-3 was 99.1% similar to Halococcus agarilyticus 62E (T) of the family Halococcaceae of the domain Archaea. This study is the first evidence of retrieval and culturing of Halococcus agarilyticus strain GUGFAWS-3 (MF425611) from marine Haliclona sp. with ability to simultaneously produce protease and lipase extremozymes of ecological and biotechnological significance.


Marine Haliclona sp. Haloarchaea Protease Lipase Extremozymes 



S. K. Gaonkar gratefully acknowledges Goa University, research studentship award.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13213_2018_1391_MOESM1_ESM.docx (181 kb)
ESM 1 (DOCX 180 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan 2018

Authors and Affiliations

  • Sanket Krishnanath Gaonkar
    • 1
  • Irene Jeronimo Furtado
    • 1
    Email author
  1. 1.Department of MicrobiologyGoa UniversityTaleigao PlateauIndia

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