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Pig farm environment as a source of beta-lactamase or AmpC-producing Klebsiella pneumoniae and Escherichia coli

  • Arindam Samanta
  • Achintya Mahanti
  • Souvick Chatterjee
  • Siddhartha Narayan Joardar
  • Samiran Bandyopadhyay
  • Tapas Kumar Sar
  • Guru Prasad Mandal
  • Tapan Kumar Dutta
  • Indranil Samanta
Original Article
  • 17 Downloads

Abstract

The present study was undertaken to detect the occurrence of beta-lactamase-/AmpC-producing Klebsiella and Escherichia coli in healthy pigs, feed, drinking water, and pen floor or surface soil. The study also intended to detect the clonal relationship between the environmental and porcine isolates to confirm the route of transmission. Rectal swabs and environmental samples were collected from apparently healthy pigs kept in organized or backyard farms in India. The pigs had no history of antibiotic intake. Production of phenotypical beta-lactamase, associated genes, and class I integron gene was detected in E. coli and Klebsiella isolates. The phylogenetic relationship among the isolates was established on the basis of Random amplification of polymorphic DNA banding pattern. Beta-lactamase-producing Klebsiella were isolated from healthy pigs (20.0%), pen floor swabs/surface soil swabs (14.0%), and drinking water (100%). Escherichia coli isolated from healthy pigs (14.4%), pen floor/surface soil (8.0%), and drinking water (33.3%) were detected as beta-lactamase producers. Majority of beta-lactamase-producing isolates possessed blaCTX-M-9. Further, 35 (81%) Klebsiella and all the E. coli isolates were detected as AmpC beta-lactamase ACBL producers and possessed blaAmpC. Sixteen beta-lactamase-producing Klebsiella (37.20%) and 13 E. coli (86.67%) possessed class I integron. Few resistant isolates from environmental sources (surface soil swab and drinking water) and the studied pigs were detected within the same cluster of the dendrogram representing their similarities. The study indicated about the possible role of contaminated environment as a source of beta-lactamase/AmpC-producing Klebsiella and E. coli in pigs.

Keywords

ESBL E. coli Environment India Klebsiella Pig 

Notes

Acknowledgements

The authors provide sincere thanks to honorable Vice Chancellor, West Bengal University of Animal and Fishery Sciences for the infrastructure and facilities. We also acknowledge the Director, Central Research Institute, Kasuli, HP, India for serogrouping of E. coli isolates.

Funding

The study was partially funded by Department of Biotechnology, Government of India (grant no.: BT/PR16149/NER/95/85/2015) and West Bengal State Council for Science and Technology (grant no.: 929(Sanc.)/ST/P/S and T/ 1G-25/2016).

Compliance with ethical standards

The study was approved by Institutional Biosafety Committee.

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals (if applicable)

Not applicable.

Informed consent

Not applicable.

Supplementary material

13213_2018_1387_MOESM1_ESM.doc (36 kb)
ESM 1 (DOC 36 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan 2018

Authors and Affiliations

  • Arindam Samanta
    • 1
  • Achintya Mahanti
    • 1
  • Souvick Chatterjee
    • 1
  • Siddhartha Narayan Joardar
    • 1
  • Samiran Bandyopadhyay
    • 2
  • Tapas Kumar Sar
    • 3
  • Guru Prasad Mandal
    • 4
  • Tapan Kumar Dutta
    • 5
  • Indranil Samanta
    • 1
  1. 1.Department of Veterinary MicrobiologyWest Bengal University of Animal and Fishery SciencesKolkataIndia
  2. 2.Eastern Regional StationICAR-Indian Veterinary Research InstituteKolkataIndia
  3. 3.Department of Veterinary Pharmacology and ToxicologyWest Bengal University of Animal and Fishery SciencesKolkataIndia
  4. 4.Department of Animal NutritionWest Bengal University of Animal and Fishery SciencesKolkataIndia
  5. 5.Department of Veterinary MicrobiologyCentral Agricultural UniversityAizawlIndia

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