Low-cost effective culture medium optimization for d-lactic acid production by Lactobacillus coryniformis subsp. torquens under oxygen-deprived condition
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Lactic acid is considered a commodity and its production is boosted by the synthesis of polylactic acid. d-lactic acid (DLA) isomer offers greater flexibility and biodegradability and it can only be obtained in its pure form through fermentation. The lactate dehydrogenase is stereospecific for homofermentative production of DLA isomer in the metabolic pathway of Lactobacillus coryniformis subsp. torquens, with optical purity of ≥ 99.9% under oxygen-deprived condition. A simple culture medium that increases DLA production and reduces fermentation costs is fundamental for industrial applicability. A central composite rotatable design was used to evaluate significant components influencing the DLA production. Concentrations were adjusted using the Design-Expert 7.0 optimization tool with a desirability coefficient of 0.693 and the best concentrations of each component were determined. Finally, an assay in the bioreactor with the modified culture medium resulted in a product yield of 0.95 g/g, volumetric productivity of 0.85 g/L.h and 95% of efficiency.
KeywordsFermentation Optical purity Central composite rotatable design Optimization tool
The authors gratefully acknowledge financial support by Leopoldo Américo Miguez de Mello Research and Development Center (Cenpes), Petrobras. We also would like to acknowledge the support of the Laboratories of Bioprocess Development (Ladebio), from the school of chemistry of Federal University of Rio de Janeiro, UFRJ.
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Conflict of interest
The authors declare that they have no conflict of interest.
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