Annals of Microbiology

, Volume 68, Issue 9, pp 547–555 | Cite as

Low-cost effective culture medium optimization for d-lactic acid production by Lactobacillus coryniformis subsp. torquens under oxygen-deprived condition

  • Lizeth JaramilloEmail author
  • Danielle Santos
  • Elcio Borges
  • Diogo Dias
  • Nei Pereira
Original Article


Lactic acid is considered a commodity and its production is boosted by the synthesis of polylactic acid. d-lactic acid (DLA) isomer offers greater flexibility and biodegradability and it can only be obtained in its pure form through fermentation. The lactate dehydrogenase is stereospecific for homofermentative production of DLA isomer in the metabolic pathway of Lactobacillus coryniformis subsp. torquens, with optical purity of ≥ 99.9% under oxygen-deprived condition. A simple culture medium that increases DLA production and reduces fermentation costs is fundamental for industrial applicability. A central composite rotatable design was used to evaluate significant components influencing the DLA production. Concentrations were adjusted using the Design-Expert 7.0 optimization tool with a desirability coefficient of 0.693 and the best concentrations of each component were determined. Finally, an assay in the bioreactor with the modified culture medium resulted in a product yield of 0.95 g/g, volumetric productivity of 0.85 g/L.h and 95% of efficiency.


Fermentation Optical purity Central composite rotatable design Optimization tool 



The authors gratefully acknowledge financial support by Leopoldo Américo Miguez de Mello Research and Development Center (Cenpes), Petrobras. We also would like to acknowledge the support of the Laboratories of Bioprocess Development (Ladebio), from the school of chemistry of Federal University of Rio de Janeiro, UFRJ.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan 2018

Authors and Affiliations

  • Lizeth Jaramillo
    • 1
    Email author
  • Danielle Santos
    • 2
  • Elcio Borges
    • 3
  • Diogo Dias
    • 1
  • Nei Pereira
    • 3
  1. 1.Laboratory of Biocorrosion, Biodegradation and Biosynthesis, School of ChemistryFederal University of Rio de JaneiroRio de JaneiroBrazil
  2. 2.Nucleus of Ecology and Socio-Environmental Development of Macaé, NUPEMFederal University of Rio de JaneiroMacaéBrazil
  3. 3.Department of Biochemical Engineering. School of ChemistryFederal University of Rio de JaneiroRio de JaneiroBrazil

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