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Annals of Microbiology

, Volume 67, Issue 4, pp 321–331 | Cite as

Isolation and characterization of anticancer flavone chrysin (5,7-dihydroxy flavone)-producing endophytic fungi from Passiflora incarnata L. leaves

  • Prabukumar Seetharaman
  • Sathishkumar Gnanasekar
  • Rajkuberan Chandrasekaran
  • Gobinath Chandrakasan
  • Murugan Kadarkarai
  • Sivaramakrishnan Sivaperumal
Original Article

Abstract

Chrysin (5,7-dihydroxy flavone, ChR) is a flavone of plant origin, possessing numerous biomedical properties, such as antimicrobial, anti-inflammatory, anti-diabetic, anxiolytic, hepatoprotective, anti-aging and anticonvulsant activities. In this study, chrysin-producing fungal endophytes (A. alternata KT380662, C. capsici KT373967, and C. taiwanense PI-3 KX580307) were isolated from the leaves of Passiflora incarnata L. and characterised via morphology and internal transcribed spacer (ITS) sequences. Thin layer chromatography and high-performance liquid chromatography profiles of fungal extracts showed Rf values and retention times that closely match those of standard chrysin (ChR). Further, the production of fungal chrysin (FChR) was confirmed through UV-vis spectroscopy, FT-IR, LC-ESI-MS, and 1H1 NMR analysis. Among the isolated strains, A. alternata KT380662 was identified as having a high-level of ChR production, with rates measuring approximately 846 mg L−1. On the other hand, in vitro anticancer and radical scavenging studies proved that FChR has significant cytotoxic activity against human liver carcinoma cells (HepG2). These results clearly imply that the isolated A. alternata KT380662 could serve as an alternative source for the commercial production of ChR, which holds anticancer and radical scavenging activities, and the fungal-derived ChR can be used in chemotherapy or in prodrug development.

Keywords

Chrysin (5,7-dihydroxy flavone ChR) Endophytic fungi Passiflora incarnata L. Alternaria alternata KT380662 LC-ESI-MS Lung cancer 

Notes

Acknowledgements

The authors thank the Department of Science and Technology Promotion of University Research and Scientific Excellence (DST-PURSE; DST Sanction Order No- SR/FT/LS- 113/2009) for providing confocal microscopy images, Central Drug Research Institute Sophisticated Analytical Instrument Facilities (CDRI-SAIF), Lucknow for the LC-ESI-MS facility, and the Indian Institute of Science (IISc), Bangalore for the NMR facility.

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Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2017

Authors and Affiliations

  • Prabukumar Seetharaman
    • 1
  • Sathishkumar Gnanasekar
    • 1
  • Rajkuberan Chandrasekaran
    • 1
  • Gobinath Chandrakasan
    • 1
  • Murugan Kadarkarai
    • 2
  • Sivaramakrishnan Sivaperumal
    • 1
  1. 1.Department of BiotechnologyBharathidasan UniversityTiruchirappalliIndia
  2. 2.Vice-ChancellorThiruvalluvar UniversityVelloreIndia

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