Annals of Microbiology

, Volume 67, Issue 2, pp 157–163 | Cite as

Co-fermentation of cassava waste pulp hydrolysate with molasses to ethanol for economic optimization

  • Thippawan Wattanagonniyom
  • Wen-Chien Lee
  • Vasana Tolieng
  • Somboon Tanasupawat
  • Ancharida Akaracharanya
Original Article

Abstract

Cassava waste pulp (CWP)–enzymatic hydrolysate was co-fermented with molasses (CWP-EH/molasses mixture) with the aim to optimize ethanol production by Saccharomyces cerevisiae TISTR 5606 (SC 90). The optimal fermentation conditions for ethanol production using this mixture were 245 g/L initial total sugar supplemented with KH2PO4 (8 g/L), at 30 °C for 48 h of fermentation under an oxygen-limited condition with agitation at 100 rpm, producing an ethanol concentration of 70.60 g/L (0.31 g ethanol/g total sugar). The addition of cassava tuber fiber (solid residue of CWP after enzymatic hydrolysis) at 30 g/L dry weight to the CWP-EH/molasses mixture increased ethanol production to 74.36 g/L (0.32 g ethanol/g total sugar). Co-fermentation of CWP-EH with molasses had the advantage of not requiring any supplementation of the fermentation mixture with reduced nitrogen.

Keywords

Cassava waste pulp Co-fermentation Molasses Ethanol Saccharomyces cerevisiae 

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Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2016

Authors and Affiliations

  • Thippawan Wattanagonniyom
    • 1
  • Wen-Chien Lee
    • 2
  • Vasana Tolieng
    • 3
  • Somboon Tanasupawat
    • 4
  • Ancharida Akaracharanya
    • 1
  1. 1.Department of Microbiology, Faculty of ScienceChulalongkorn UniversityPathumwanThailand
  2. 2.Department of Chemical EngineeringNational Chung Cheng UniversityMinhsiungTaiwan
  3. 3.Institute of Biotechnology and Genetic EngineeringChulalongkorn UniversityPathumwanThailand
  4. 4.Department of Biochemistry and Microbiology, Faculty of Pharmaceutical ScienceChulalongkorn UniversityPathumwanThailand

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