Annals of Microbiology

, Volume 66, Issue 3, pp 1049–1055 | Cite as

L-phenylacetylcarbinol production by yeast petite mutants

  • Mohsen Doostmohammadi
  • Mohammad Ali Asadollahi
  • Iraj Nahvi
  • Davoud Biria
  • Gholam Reza Ghezelbash
  • Maryam Kheyrandish
Original Article
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Accepted:
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Abstract

The yeast Saccharomyces cerevisiae is able to biotransform benzaldehyde into L-phenylacetylcarbinol (L-PAC), a key intermediate in the production of ephedrine and pseudoephedrine, by the action of pyruvate decarobxylase (PDC) enzyme. This biotransformation can alternatively be performed by acetohydroxyacid synthase (AHAS) which is a mitochondrial enzyme. In the yeast petite mutants, AHAS accumulates in the cytosol. In the current study, wild-type yeast cells and yeast petite mutants were examined for L-PAC biosynthesis. The results showed higher L-PAC titers in the yeast petite mutants. In addition, the effect of cell immobilization and carbon source (glucose or molasses) on L-PAC production was investigated. It was found that cell immobilization enhances L-PAC formation. The highest L-PAC concentration (2.4 g/l) was obtained at 2 g/l of benzaldehyde using the immobilized petite mutants grown on molasses.

Keywords

L-Phenylacetylcarbinol Acetohydroxyacid synthase Saccharomyces cerevisiae Petite mutants Immobilization Benzaldehyde 
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Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2016

Authors and Affiliations

  • Mohsen Doostmohammadi
    • 1
  • Mohammad Ali Asadollahi
    • 1
  • Iraj Nahvi
    • 2
  • Davoud Biria
    • 1
  • Gholam Reza Ghezelbash
    • 3
  • Maryam Kheyrandish
    • 1
  1. 1.Department of Biotechnology, Faculty of Advanced Sciences and TechnologiesUniversity of IsfahanIsfahanIran
  2. 2.Department of Biology, Faculty of ScienceUniversity of IsfahanIsfahanIran
  3. 3.Department of Biology, Faculty of ScienceShahid Chamran UniversityAhvazIran

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