Laboratory maintained and wild populations of Hydra differ in their microbiota
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Very often, the microbial composition of lab-maintained model organisms is significantly different compared to their natural counterparts. Controlled environment in the lab versus dynamic conditions in the wild could be one of the reasons for such differences. Considering the impact of microbes on development, immunology, ecology, evolution, etc., it is very important to explore the microbial structure of the host from both lab and wild populations. In this study we have explored the microbiota of H. vulgaris collected from two localities in India as well as lab-maintained populations, using Ion torrent PGM. All samples were dominated by phylum Proteobacteria; however, at a deeper taxonomic level, microbial structure of the samples collected from different localities showed notable differences among them and the lab-maintained population as well. Knowledge of such variation in microbiota of the host could aid in our understanding of host–microbiota interaction.
KeywordsEnvironmental influence Hydra Microbial diversity Next Generation Sequencing
This study was funded by extramural grants from the Department of Science and Technology (DST), Government of India to Surendra Ghaskadbi and a DST-PURSE grant to Saroj Ghaskadbi. YSS wishes to thank Department of Biotechnology (DBT), Government of India for financial support. We would like to thank Dr. Nachiket Marathe and Dr. Om Prakash Sharma for their valuable comments.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Caporaso JGJ, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336CrossRefPubMedPubMedCentralGoogle Scholar
- Collins SM (2014) A role for the gut microbiota in IBS. Gastroenterol Hepatol 497–505Google Scholar
- Franzenburg S, Walter J, Künzel S, Wang J, Baines JF, Bosch TCG, Fraune S (2013) Distinct antimicrobial peptide expression determines host species-specific bacterial associations. PNAS E3730–E3738Google Scholar
- Franzenburg S, Fraune S, Altrock PM, Künzel S, Baines JF, Traulsen A, Bosch TCG (2014) Bacterial colonization of Hydra hatchlings follows a robust temporal pattern. ISME J 7(4):1–10Google Scholar
- Fraune S, Augustin R, Bosch TCG (2009) Exploring host microbe interaction in Hydra. Microbe 4:457–462Google Scholar
- Fraune S, Anton-Erxleben F, Augustin R, Franzenburg S, Knop M, Schroder K, Willoweit-Ohl D, Bosch TCG (2014) Bacteria–bacteria interactions within the microbiota of the ancestral metazoan Hydra contribute to fungal resistance. ISME 1–14Google Scholar
- Grasis JA, Lachnit T, Anton-Erxleben F, LIm YW, Schmieder R, Fraune S, Franzenburg S, Insua S, Machado G, Haynes M, Littile M, Kimble R, Rosenstiel P, Rohwer FL, Bosch TC (2014) Species specific viriomes in the ancestral holobiont Hydra. Plos One 9(10):e109952CrossRefPubMedPubMedCentralGoogle Scholar
- O’connor TK, Humphrey PT, Lapoint RT, Whiteman NK, O’Grady PM (2014) Microbial interaction and the ecology and evolution of Hawaiin Drosophildae. Front Microbiol 5(616):1–8Google Scholar
- Reddy PC, Barve A, Ghaskadbi S (2011) A description and phylogenetic characterization of common Hydra from India. Curr Sci 101:736–738Google Scholar
- Schloss PD, Handelsman J (2005) Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Appl Environ Microbiol 1501–1506Google Scholar
- Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75(23):7537–7541CrossRefPubMedPubMedCentralGoogle Scholar
- Sullam KE, Rubin BER, Dalton CM, Kilham SS, Flecker AS, Russell JA (2015) Divergence across diet, time and populations rules out parallel evolution in the gut microbiomes of Trinidadian guppies. ISME 1–15Google Scholar