Annals of Microbiology

, Volume 66, Issue 2, pp 559–568 | Cite as

Exploiting of honey-associated Bacillus strains as plant-growth promoting bacteria for enhancing barley growth in rare earth tailings

  • Yanzhou Zhang
  • Haiye Luan
  • Zhiwen Wei
  • Zhikui Hao
  • Ruchun Xi
  • Xiangru LiaoEmail author
Original Article


In the coming decades, exploitation of plant growth promoting bacteria (PGPB) will be a major trend in sustainable agriculture. Except for rhizobacteria, the bacteria inhabiting the non-rhizosphere could be considered the supplementary microbial source to exploit PGPB. Rare earth tailings soil (RETS) has caused a serious threat to sustainable agriculture due to excessive aluminum (Al) toxicity under acidic condition (pH < 5.5). The efficient restoration strategy is to combine phytoremediation and PGPB. The aim of this work was to screen honey-associated Bacillus strains as PGPB to improve barley (Hordeum vulgare L.) growth in RETS. Nine Bacillus strains from honey were initially selected with higher tolerance to acid (pH 4.0) and Al stress (1.5 mM). Only six strains among these nine strains could produce ACC deaminase. The nine strains were all efficient in dissolving phosphate, producing organic acid, siderophore, and IAA. The nine strains could promote barley seedlings growth mainly via production of organic acid, ACC deaminase. However, this growth promotion was weakened slightly by siderophore and phosphate solubilization under acidic condition. On the whole, inoculation with the nine strains obviously enhanced barley seedlings growth in RETS. Thus, the nine honey-associated Bacillus strains could be exploited as PGPB to enhance barley growth in RETS.


PGPB PGPP Honey-associated Bacillus Barley Rare earth tailings soil 



This work was supported by the Fundamental Research Funds for the Central Universities. The research was funded by Collaborative Innovation Involving Production, Teaching & Research funds of Jiangsu Province (BY2014023-28). This work was also supported by grants from Jiangsu Agriculture Science & Technology Innovation Fund (JASTIF),SCX(14)5076.

Supplementary material

13213_2015_1135_MOESM1_ESM.docx (18 kb)
Supplementary Table 1 (DOCX 17 kb)
13213_2015_1135_MOESM2_ESM.docx (20 kb)
Supplementary Table 2 (DOCX 20 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2015

Authors and Affiliations

  • Yanzhou Zhang
    • 1
  • Haiye Luan
    • 2
  • Zhiwen Wei
    • 1
  • Zhikui Hao
    • 3
  • Ruchun Xi
    • 4
  • Xiangru Liao
    • 1
    Email author
  1. 1.The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina
  2. 2.Institute of Agricultural Science in Jiangsu Coastal AreasYanchengChina
  3. 3.Institute of Applied BiotechnologyTaizhou Vocational & Technical CollegeTaizhouChina
  4. 4.College of Forestry, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant GermplasmSouth China Agricultural UniversityGuangzhouChina

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