Annals of Microbiology

, Volume 65, Issue 4, pp 2081–2089 | Cite as

A higher in value biopolymer product of polyhydroxyalkanoates (PHAs) synthesized by Alcaligenes latus in batch/repeated batch fermentation processes of sugar cane juice

Original Article
First Online:
Received:
Accepted:

Abstract

Biodegradable polymers of polyhydroxyalkanoates (PHAs) are produced and accumulated in various microorganisms, especially in bacteria. In this work, a cheap agricultural sugar cane juice (SCJ) was used as a carbon source by Alcaligenes latus during its growth coupled with the production of PHAs via batch/ repeated batch fermentation processes. In batch fermentation, the maximum PHAs and PHAs content of 2.20 ± 0.14 g/L and 53.65 % were obtained within 24 h while in the case of four cycles of repeated batch fermentation, the PHAs productivity was reached at about 0.21 g/L·h that was 2.33 fold when compared to the first batch fermentation (0.09 g/L·h). Interestingly, the duration in each cycle or in batch operation during repeated batch fermentation was shorter than that found in the batch fermentation and PHAs productivity was also improved. After PHAs recovery, the physicochemical properties of nuclear magnetic resonance (NMR) and a fourier transform infrared (FTIR) spectrogram were characterized and revealed to be in full agreement with the standard polymer of polyhydroxybutyrate (PHB).

Keywords

Polyhydroxyalkanoates Alcaligenes latus Sugar cane juice Fermentation process 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

One of the authors (P. Singhaboot) would like to sincerely acknowledge the Graduate School of Khon Kaen University, Khon Kaen, Thailand for a financial contribution (The Research Fund for Supporting Lecturer to Admit a High Potential Student to Study and Research on His Expert Program : Contract project no. 531T101) and a travel abroad bursary, while P. Kaewkannetra also would like to sincerely thank the Thailand Research Fund (TRF) (Contract no.DBG 5380013) and the National Research University (NRU) Project of Khon Kaen University for matching funds.

References

  1. Braunegg G, Bogensberger B (1985) For the kinetics of growth and the storage of poly-D (−)-3-hydroxybutyric acid by Alcaligenes latus. Acta Biotechnol 5:339–345CrossRefGoogle Scholar
  2. El-Sayed AA, Abdelhady HM, Abdel Hafez AM, Khodair TA (2009) Batch production of polyhydroxybutyrate (PHB) by Ralstonia eutropha and Alcaligenes latus using bioreactor different culture strategies. J Appl Sci Res 5:556–564Google Scholar
  3. Grothe E, Moo-Young M, Chisti Y (1999) Fermentation optimization for the production of (hydroxybutyric acid) microbial thermoplastic. Enzym Microb Technol 25:132–141CrossRefGoogle Scholar
  4. Hangii UJ (1990) Pilot scale production PHB with Alcaligenes latus. In: Dawes EA (ed) Novel biodegradable microbial polymer. Kluwer Academic Publishers, Dordrecht, pp 65–70CrossRefGoogle Scholar
  5. Hrabak O (1992) Industrial production poly-β-hydroxybutyrate. FEMS Microbiol Rev 103:251–256Google Scholar
  6. Huang WC, Chen SJ, Chen TL (2008) Production of hyaluronic acid by repeated batch fermentation. Biochem Eng J 40:460–464CrossRefGoogle Scholar
  7. Kaewkannetra P, Tanonkeo P, Tanamool V, Imai T (2008) Biorefinery of squeeze sweet sorghum juice into value added product of biopolymer. J Biotechnol 136:412CrossRefGoogle Scholar
  8. Khanna S, Srivastava AK (2005a) Recent advances in microbial polyhydroxyalkanoates. Process Biochem 40:607–619CrossRefGoogle Scholar
  9. Khanna S, Srivastava AK (2005b) Repeated batch cultivation of Ralstonia eutropha for poly (β- hydroxybutyrate) production. Biotechnol Lett 27:1401–1403CrossRefPubMedGoogle Scholar
  10. Kim BS, Lee SC, Lee SY, Chang HN, Chang YK, Woo SI (1994) Production of poly (3-hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control. Biotechnol Bioeng 43:892–898CrossRefPubMedGoogle Scholar
  11. Kim SW, Kim P, Lee HS, Kim JH (1996) High production of poly-β-hydroxybutyrate (PHB) from Methylobacterium organophilum under potassium limitation. Biotechnol Lett 18:25–30CrossRefGoogle Scholar
  12. Kumalaningsih S, Nur H, Nur A (2011) Optimization of polyhydroxyalkanoates (PHA) production from liquid bean curd waste by Alcaligenes latus bacteria. J Agric Food Technol 1:63–67Google Scholar
  13. Kunasundari B, Sudesh K (2011) Isolation and recovery of microbial polyhydroxyalkanoates. eXPRESS Polym Lett 5:620–634CrossRefGoogle Scholar
  14. Lee SY (1996a) Bacterial polyhydroxyalkanoates. Biotechnol Bioeng 49:1–14CrossRefPubMedGoogle Scholar
  15. Lee SY (1996b) Plastic bacteria? Progress and prospects for polyhydroxyalkanoate production in bacteria. Trends Biotechnol 14:431–438CrossRefGoogle Scholar
  16. Lee SY (1997) E. coli moves into the plastic age. Nat Biotechnol 15:17–18Google Scholar
  17. Lee SY, Chang HN (1995) Production of poly (3-hydroxybutyric acid) by recombinant Escherichia coli strains: genetic and fermentation studies. Can J Microbiol 41:207–215CrossRefPubMedGoogle Scholar
  18. Lee SY, Yim KS, Chang HN, Chang YK (1994) Construction of plasmids, estimation of plasmid stability, and use of stable plasmids for the production of poly (3-hydroxybutyric acid) in Escherichia coli. J Biotechnol 32:203–211CrossRefPubMedGoogle Scholar
  19. Morimura S, Ling ZY, Kida K (1997) Ethanol production by repeated batch fermentation at high temperature in a molasses medium containing a high concentration of total sugar by a thermotolerant flocculating yeast with improved salt tolerance. J Ferment Bioeng 83:271–274CrossRefGoogle Scholar
  20. Page WJ, Cornish A (1993) Growth of Azotobacter vinelandii UWD in fish peptone medium and simplified extraction of poly-β-hydroxybutyrate. Appl Environ Microbiol 59:4236–4244PubMedCentralPubMedGoogle Scholar
  21. Pandey A, Soccol CR, Nigam P, Soccol VT (2000) Biotechnological potential of agro-industrial residues. I: sugarcane bagasse. Bioresour Technol 74:69–80CrossRefGoogle Scholar
  22. Park YS, Toda K, Fukaya M, Okumura H, Kawamura Y (1991) Production of a high concentration acetic acid by Acetobacter aceti using a repeated fed-batch culture with cell recycling. Appl Microbiol Biotechnol 35:149–153Google Scholar
  23. Poirier Y, Nawrath C, Somerville C (1995) Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers, in bacteria and plant. Biotechnology (NY) 13:142–150CrossRefGoogle Scholar
  24. Santhanam A, Sasidharan S (2010) Microbial production of polyhydroxyalkanotes (PHA) from Alcaligenes spp. and Pseudomonas oleovorans using different carbon sources. Afr J Biotechnol 9:3144–3150Google Scholar
  25. Suwannasing W, Mooamart S, Keawkannetra P (2011) Yields of polyhydroxyalkanoates (PHAs) during batch fermentation of sugar cane juice by Alcaligenes latus and Alcaligenes eutrophus. J Life Sci 5:960–966Google Scholar
  26. Suzuki T, Yamane T, Shimizu S (1986) Mass production of poly-β-hydroxybutyric acid produced in fed-batch culture with controlled carbon/nitrogen feeding. Appl Microbiol Biotechnol 24:370–374CrossRefGoogle Scholar
  27. Tanamool V, Imai T, Danvirutai P, Kaewkannetra P (2013) An alternative approach to the fermentation of sweet sorghum juice into biopolymer of poly-β-hydroxyalkanoates (PHAs) by newly isolated, Bacillus aryabhattai PKV01. Biotechnol Bioprocess Eng 18:65–74CrossRefGoogle Scholar
  28. Yamane T (1993) Yield of poly-D-(−)-3-hydroxybytyrate from various carbon sources a theoretical study. Biotechnol Bioeng 41:165–170CrossRefPubMedGoogle Scholar
  29. Yamane T, Fukunaga M, Lee YW (1996) Increased PHB productivity by high cell density fed batch culture of Alcaligenes latus, a growth-associated PHB producer. Biotechnol Bioeng 50:197–202CrossRefPubMedGoogle Scholar
  30. Yezza A, Halasz A (2007) Production of polyhydroxybutyrate (PHB) by Alcaligenes latus from maple sap. Biotechnol Prod Proc Eng 77:269–274Google Scholar
  31. Yin P, Yahiro K, Ishigaki T, Park Y, Okabez M (1998) L(+)-Lactic acid production by repeated batch culture of Rhizopus oryzae in air lift bioreactor. J Ferment Bioeng 85:96–100Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2015

Authors and Affiliations

  1. 1.Graduate School of Khon Kaen University, Department of Biotechnology, Faculty of TechnologyKhon Kaen UniversityKhon KaenThailand
  2. 2.Department of Biotechnology, Faculty of TechnologyKhon Kaen UniversityKhon KaenThailand

Personalised recommendations