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Annals of Microbiology

, Volume 65, Issue 4, pp 1993–1999 | Cite as

Prevalence and characterization of Escherichia coli O157 and O157:H7 in retail fresh raw meat in South China

  • Shuhong Zhang
  • Xuemei Zhu
  • Qingping WuEmail author
  • Jumei Zhang
  • Xiaoke Xu
  • Haigang Li
Original Article

Abstract

Escherichia coli O157 is an important food-borne pathogen that can cause diarrhoea, haemorrhagic colitis, and haemolytic uraemic syndrome. The aim of this study was to investigate the prevalence, virulence genes, antibiotic resistance, and genetic diversity of E. coli O157 and O157:H7 in retail fresh raw meat sold in the markets of South China. Of 551 samples collected, 21 (3.81 %) were contaminated with E. coli O157 and seven (1.27 %) with O157:H7. The highest prevalence rate was found in beef (13.32 %), followed by pork (6.90 %), chicken (3.28 %), duck (2.54 %), and mutton (0). The virulence genes stx1, stx2, eaeA, and hlyA were detected in 10.71, 21.43, 85.71, and 25 % of 28 isolates, respectively. The isolates were highly resistant to penicillin (100 %), chloramphenicol (64.29 %), ampicillin (57.14 %), and sensitive to gentamicin (100 %), cefotazidime (96.43 %), and ciprofloxacin (96.43 %). Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) classified 28 isolates and two reference strains into 19 different profiles with a discrimination index (D) of 0.961. Four E. coli O157:H7 isolates from beef showed 83 % similarity with the two clinical reference strains, indicating a potential high virulence for consumers. The results of this study suggested that fresh raw meat could be potential vehicles for transmission of E. coli O157 to humans.

Keywords

Prevalence Virulence genes Antimicrobial resistance Enterobacterial repetitive intergenic consensus-PCR Escherichia coli O157 O157:H7 

Notes

Acknowledgments

This work was supported by research grants from the International Science and Technology Cooperation Projects (No.2013DFH30070). The authors acknowledge colleagues in our lab for kindly technical assistance. We are also thankful to the reviewers for their valuable suggestions and comments.

Supplementary material

13213_2015_1037_Fig2_ESM.gif (79 kb)
Supplemental files Figure S1

These are the results of multiple-PCR for identification of E .coli O157 and E. coli O157:H7 isolates. 1(a) Lanes: M, DNA size marker (DL2000); CK, negative control; 1, positive control ATCC35150; Lanes 2–8 are E. coli O157:H7 isolates (172–3, 202–2, 072–2, 075–1, 049–2, 050–2, 703–1). 1(b) Lanes: M, DNA size marker (DL2000); CK, negative control; 1, positive control ATCC35150; Lanes 2–22 are E. coli O157 isolates (1501–4, 142–2, 056–2, 230–3, 242–2, 2253–3, 2372–1, 2453–1, 073–3, 078–2, 236–1, 1552–1,054-1, 055–2, 009–1, 028–2, 175–4, 241–2, 225–2, 240–3, 016–3) (GIF 78 kb)

13213_2015_1037_MOESM1_ESM.tif (589 kb)
High Resolution Image (TIFF 588 kb)
13213_2015_1037_Fig3_ESM.gif (228 kb)
Supplemental files Figure S2

The geographic locations of the sampling sites in China. (GIF 228 kb)

13213_2015_1037_MOESM2_ESM.tif (357 kb)
High Resolution Image (TIFF 357 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2015

Authors and Affiliations

  • Shuhong Zhang
    • 1
  • Xuemei Zhu
    • 1
  • Qingping Wu
    • 1
    Email author
  • Jumei Zhang
    • 1
  • Xiaoke Xu
    • 1
  • Haigang Li
    • 1
  1. 1.State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Public Laboratory for Applied and New Technology of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and ApplicationGuangdong Institute of MicrobiologyGuangzhouChina

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