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Annals of Microbiology

, Volume 65, Issue 4, pp 1941–1948 | Cite as

Lipid production by the filamentous cyanobacterium Limnothrix sp. growing in synthetic wastewater in suspended- and attached-growth photobioreactor systems

  • Christina N. EconomouEmail author
  • Nikolaos Marinakis
  • Maria Moustaka-Gouni
  • George Kehayias
  • George Aggelis
  • Dimitris V. Vayenas
Original Article

Abstract

The main objective of this study was the production of biotechnological oil/biodiesel from a filamentous cyanobacterium Limnothrix sp. with simultaneous treatment of a model wastewater. A novel attached-growth photobioreactor was designed to facilitate the harvesting of cyanobacterial biomass and to maximize biomass and lipid production compared to suspended-growth cultivation systems. Kinetic experiments with different initial nitrate and phosphate concentrations were performed in both suspended- and attached-growth cultivation modes to define the biomass and lipid concentration as well as the capability of Limnothrix sp. to remove nutrients from the artificial wastewater. The removal of nitrate and phosphate was high in both suspended- and attached-growth systems. The results of this study also demonstrated that the proposed attached-growth photobioreactor system ensured higher biomass productivity compared to the suspended-growth cultivation system. The absence of long aliphatic chain fatty acids as well as the high amount of saturated and monounsaturated fatty acids (almost 80 %) in cyanobacterial lipid make the oil produced a promising feedstock for biodiesel production.

Keywords

Cyanobacteria Lipids Wastewater Limnothrix sp. Attached-growth system Novel photobioreactor 

References

  1. Adey WH, Kangas PC, Mulbry W (2011) Algal turf scrubbing: Cleaning surface waters with solar energy while producing a biofuel. Bioscience 61:434–441CrossRefGoogle Scholar
  2. AFNOR, 1984 Recueil des normes francΈ aises des corps gras, grains olιagineux et produits dιrives. In: Association FrancΈ aise pour Normalisation, 3e’me (ed.). Paris, p. 95Google Scholar
  3. Akoto L, Pel R, Irth H, Brinkman UAT, Vreuls RJJ (2005) Automated GC–MS analysis of raw biological samples application to fatty acid profiling of aquatic micro-organisms. J Anal Appl Pyrolysis 73:69–75CrossRefGoogle Scholar
  4. Amaro HM, Guedes AC, Malcata FX (2011) Advances and perspectives in using microalgae to produce biodiesel. Appl Energ 88:3402–3410CrossRefGoogle Scholar
  5. APHA, AWWA and WPCF (1998) Standard methods for the examination of water and wastewater, 20th edn.Washington, DCGoogle Scholar
  6. Bellou S, Aggelis G (2012) Biochemical activities in Chlorella sp. and Nannochloropsis salina during lipid and sugar synthesis in a lab-scale open pond simulating reactor. J Biotech 164:318–329CrossRefGoogle Scholar
  7. Bellou S, Baeshen MN, Elazzazy AM, Aggeli D, Sayegh F, Aggelis G (2014) Microalgal lipids biochemistry and biotechnological perspectives. Biotechnol Adv 32:1476–1493CrossRefPubMedGoogle Scholar
  8. Castenholz RW (2001) General characteristics of the cyanobacteria. In: Boone DR, Castenholz RW (eds) Bergey’s manual of systematic bacteriology, 2nd edn, vol.1. Springer, New York, pp 474–487Google Scholar
  9. Chalkia E, Kehayias G (2013) Zooplankton community dynamics and environmental factors in Lake Ozeros (Greece). Mediterranean Marine Sci 14(3):32–41CrossRefGoogle Scholar
  10. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306CrossRefPubMedGoogle Scholar
  11. de la Noue J, Laliberte G, Proulx D (1992) Algae and waste water. J Appl Phycol 4:247–254CrossRefGoogle Scholar
  12. Demirbas A, Demirbas MF (2011) Importance of algae oil as a source of biodiesel. Energy Convers Manag 52:163–170CrossRefGoogle Scholar
  13. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  14. Economou CN, Makri A, Aggelis G, Pavlou S, Vayenas DV (2010) Semi-solid state fermentation of sweet sorghum for the biotechnological production of single cell oil. Bioresource Technol 101:1385–1388CrossRefGoogle Scholar
  15. Economou CN, Aggelis G, Pavlou S, Vayenas DV (2011) Single cell oil production from rice hulls hydrolysate. Bioresource Technol 102:9737–9742CrossRefGoogle Scholar
  16. Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:450–497Google Scholar
  17. Gkelis S, Rajaniemi P, Vardaka E, Moustaka-Gouni M, Lanaras T, Sivonen K (2005) Limnothrix redekei (Van Goor) Meffert (Cyanobacteria) strains from Lake Kastoria, Greece form a separate phylogenetic group. Microb Ecol 49:176–182CrossRefPubMedGoogle Scholar
  18. Hashimoto S, Furukawa K (1989) Nutrient removal from secondary effluent by filamentous algae. J Ferment Bioeng 67:62–69CrossRefGoogle Scholar
  19. Haury JF, Spiller H (1981) Fructose uptake on growth and nitrogen fixation by Anabaena variabilis. J Bacteriol 147:227–235PubMedCentralPubMedGoogle Scholar
  20. Hulatt CJ, Thomas DN (2010) Dissolved organic matter (DOM) in microalgal photobioreactors: a potential loss in solar energy conversion? Bioresource Technol 101:8690–8697CrossRefGoogle Scholar
  21. Johnson MB, Wen Z (2010) Development of an attached microalgal growth system for biofuel production. Appl Microbiol Biot 85:525–534CrossRefGoogle Scholar
  22. Kebede-Westhead E, Pizarro C, Mulbry WW, Wilkie AC (2003) Production and nutrient removal by periphyton grown under different loading rates of anaerobically digested flushed dairy manure. J Phycol 39:1275–1282CrossRefGoogle Scholar
  23. Kebede-Westhead E, Pizarro C, Mulbry WW (2006) Treatment of swine manure effluent using freshwater algae: production, nutrient recovery, and elemental composition of algal biomass at four effluent loading rates. J Appl Phycol 18:41–46CrossRefGoogle Scholar
  24. Markou G, Georgakakis D (2011) Cultivation of filamentous cyanobacteria (blue-green algae) in agro-industrial wastes and wastewaters: a review. Appl Energ 88:3389–3401CrossRefGoogle Scholar
  25. Marquez FJ, Sasaki K, Kakizono T, Nishio N, Nagai S (1993) Growth characteristics of Spirulina platensis in mixotrophic and heterotrophic conditions. J Ferment Bioeng 76:408–410CrossRefGoogle Scholar
  26. Mata TM, Martins AA, Caetano NS (2010) Microalgae for biodiesel production and other applications: a review. Renew Sust Energ Rev 14:217–232CrossRefGoogle Scholar
  27. Meng X, Yang J, Xu X, Zhang L, Nie Q, Xian M (2008) Biodiesel production from oleaginous microorganisms. Renew Energ 34:1–5CrossRefGoogle Scholar
  28. Moustaka-Gouni M, Vardaka E, Michaloudi E, Kormas K, Tryfon E, Mihalatou H, Gkelis S, Lanaras T (2006) Plankton food web structure in a eutrophic polymictic lake with a history of toxic cyanobacterial blooms. Limnol Oceanogr 51:715–727CrossRefGoogle Scholar
  29. Moustaka-Gouni M, Vardaka E, Tryfon E (2007) Phytoplankton species succession in a shallow Mediterranean lake (L. Kastoria, Greece). steady-state dominance of Limnothrix redekei, Microcystis aeruginosa and Cylindrospermopsis raciborskii. Hydrobiologia 575:129–140CrossRefGoogle Scholar
  30. Mulbry W, Kondrad S, Buyer J (2008) Treatment of dairy and swine manure effluents using freshwater algae: fatty acid content and composition of algal biomass at different manure loading rates. J Appl Phycol 20:1079–1085CrossRefGoogle Scholar
  31. Papanikolaou S, Aggelis G (2009) Biotechnological valorization of biodiesel derived glycerol waste through production of single cell oil and citric acid by Yarrowia lipolytica. Lipid Technol 21:83–87CrossRefGoogle Scholar
  32. Pel R, Floris V, Hoogveld H (2004) Analysis of planktonic community structure and trophic interactions using refined isotopic signatures determined by combining fluorescence-activated cell sorting and isotope-ratio mass spectrometry. Freshwater Biol 49:546–562CrossRefGoogle Scholar
  33. Piorreck M, Baasch KH, Pohl P (1984) Biomass production, total protein, chlorophylls, lipids and fatty acids of freshwater green and blue-green algae under different nitrogen regimes. Phytochemistry 23:207–216CrossRefGoogle Scholar
  34. Pizarro C, Kebede-Westhead E, Mulbry W (2002) Nitrogen and phosphorus removal rates using small algal turfs grown with dairy manure. J Appl Phycol 14:469–473CrossRefGoogle Scholar
  35. Ratledge C (2004) Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie 86:807–815CrossRefPubMedGoogle Scholar
  36. Rodjaroen S, Juntawong N, Mahakhant A, Miyamoto K (2007) High biomass production and starch accumulation in native green algal strains and cyanobacterial strains of Thailand. Kasetsart J (Nat Sci) 41:570–575Google Scholar
  37. Sassano CEN, Gioielli LA, Ferreira LS, Rodrigues MS, Sato S, Converti A, Carvalho JCM (2010) Evaluation of the composition of continuously-cultivated Arthrospira (Spirulina) platensis using ammonium chloride as nitrogen source. Biomass Bioenerg 34:1732–1738CrossRefGoogle Scholar
  38. Sialve B, Bernet N, Bernard O (2009) Anaerobic digestion of microalgae as a necessary step to make microalgal biodiesel sustainable. Biotechnol Adv 27:409–416CrossRefPubMedGoogle Scholar
  39. Thajuddin N, Subramanian G (2005) Cyanobacterial biodiversity and potential applications in biotechnology. Curr Sci 89:47–57Google Scholar
  40. Vicente G, Bautista LF, Rodriguez R, Gutierrez FJ, Sadaba I, Ruiz-Vazquez RM, Torres-Martinez S, Garre V (2009) Biodiesel production from biomass of an oleaginous fungus. Bioch Eng J 48:22–27CrossRefGoogle Scholar
  41. Wang L, Min M, Li Y, Chen P, Chen Y, Liu Y, Wang Y, Ruan R (2010) Cultivation of green algae Chlorella sp. in different wastewaters from municipal wastewater treatment plant. Appl Biochem Biotechnol 162:1174–1186CrossRefPubMedGoogle Scholar
  42. Wolk PC, Shaffer PW (1976) Heterotrophic micro- and macrocultures of a nitrogenfixing cyanobacterium. Arch Microbiol 110:145–147CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2015

Authors and Affiliations

  • Christina N. Economou
    • 1
    Email author
  • Nikolaos Marinakis
    • 1
  • Maria Moustaka-Gouni
    • 2
  • George Kehayias
    • 1
  • George Aggelis
    • 3
    • 4
  • Dimitris V. Vayenas
    • 1
    • 5
  1. 1.Department of Environmental and Natural Resources ManagementUniversity of PatrasAgrinioGreece
  2. 2.School of BiologyAristotle University of ThessalonikiThessalonikiGreece
  3. 3.Division of Genetics, Cell & Development Biology, Department of BiologyUniversity of PatrasPatrasGreece
  4. 4.Department of Biological SciencesKing Abdulaziz UniversityJeddahSaudi Arabia
  5. 5.Foundation for Research and Technology Hellas – Institute of Chemical Engineering and High Temperature Chemical ProcessesPatrasGreece

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