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Annals of Microbiology

, Volume 65, Issue 3, pp 1381–1389 | Cite as

L-Tryptophan-dependent biosynthesis of indole-3-acetic acid (IAA) improves plant growth and colonization of maize by Burkholderia phytofirmans PsJN

  • Muhammad NaveedEmail author
  • M. Amjad Qureshi
  • Zahir A. Zahir
  • M. Baqir Hussain
  • Angela Sessitsch
  • Birgit Mitter
Original Article

Abstract

Burkholderia phytofirmans PsJN is a well-known plant growth-promoting bacterium that establishes rhizospheric and endophytic colonization in different plants. PsJN inoculation promotes growth of different horticultural crops. L-Tryptophan (L-TRP) application may further improve its effectiveness, due to substrate (L-TRP)-dependent inoculum (PsJN)-derived auxins in the rhizosphere. In the present study, the substrate (L-TRP)-dependent response of PsJN inoculation to maize growth and auxin biosynthesis was evaluated under pot conditions. In vitro auxin biosynthesis by PsJN was determined in the absence and presence of L-TRP, a physiological precursor of auxins. Surface-disinfected seeds were treated with peat-based inoculum and L-TRP solutions (10−4 and 10−5 M). Results revealed that L-TRP application and PsJN inoculation, when applied separately, significantly increased the growth parameters of maize compared to untreated control. However, PsJN inoculation supplemented with L-TRP (10−5 M) gave the most promising results and significantly increased plant height, photosynthesis, chlorophyll content, root biomass and shoot biomass up to 18, 16, 45, 62 and 55 %, respectively, compared to the uninoculated control. Similarly, higher values of N, P and IAA content were observed with precursor (L-TRP)–inoculum (PsJN) interaction. The inoculant strain efficiently colonized maize seedlings and was recovered from the rhizosphere, root and shoot of plants. The results imply that substrate (L-TRP)-derived IAA biosynthesis in the rhizosphere by PsJN inoculation could be a useful approach for improving the growth, photosynthesis and nutrient content of maize plants.

Keywords

B. phytofirmans PsJN L-tryptophan Precursor–inoculum interaction Colonization Maize 

Notes

Acknowledgments

The authors gratefully acknowledge the Higher Education Commission (HEC) of Pakistan for financial support for this research.

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Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2014

Authors and Affiliations

  • Muhammad Naveed
    • 1
    • 3
    Email author
  • M. Amjad Qureshi
    • 2
  • Zahir A. Zahir
    • 1
  • M. Baqir Hussain
    • 1
  • Angela Sessitsch
    • 3
  • Birgit Mitter
    • 3
  1. 1.Institute of Soil & Environmental SciencesUniversity of Agriculture Faisalabad 3840FaisalabadPakistan
  2. 2.Soil Bacteriology SectionAyub Agriculture Research Institute (AARI)FaisalabadPakistan
  3. 3.Bioresources UnitAIT Austrian Institute of Technology GmbHTullnAustria

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